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Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection

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Abstract

Among nine chitinase-producing strains isolated from Tunisian soil, one isolate called S213 exhibited a potent chitinolytic activity. S213 strain was identified as Bacillus licheniformis by API 50CH system and sequence analysis of its partial 16S ribosomal DNA. Chitinolytic activity was induced either by colloidal chitin or fungal cell walls, and the highest chitinase activity reached at the late stationary phase exhibiting optimal temperature and pH of 50–60 °C and pH 6.0, respectively. SDS-PAGE analysis of the secreted colloidal chitin-induced proteins showed a major protein of about 65 kDa. This protein was identified as chitinase on the basis of its peptide sequences which displayed high homology with chitinase sequence of B. licheniformis ATCC 14580. Moreover, chitinolytic activity containing supernatant inhibited the growth of several phytopathogenic fungi including Phoma medicaginis. Interestingly, S213 strain reduced efficiently the damping-off disease caused by P. medicaginis in Medicago truncatula and should be envisaged in enzyme-based biopesticides against phytopathogen application.

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Acknowledgments

This work was financially supported by grants from the Tunisian Ministry of Higher Education, Scientific Research and Technologies of Information and Communication. We would like to thank Prof. Ezzedine Aouani for critically reading the manuscript.

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Authors and Affiliations

  1. Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria, BP-901, 2050, Hammam-lif, Tunisia

    Imen Ben Slimene, Olfa Tabbene, Dorra Gharbi & Ferid Limam

  2. Laboratoire des légumineuses, Centre de Biotechnologie de Borj-Cedria, BP-901, 2050, Hammam-lif, Tunisia

    Bacem Mnasri

  3. Centre de Génomique Fonctionnelle, Université de Bordeaux-Bordeaux 1 IPB, UMR 5248 CNRS-U, 146 rue Léo Saignat, 33076, Bordeaux Cedex, France

    Jean Marie Schmitter

  4. Laboratoire de Microbiologie, Université de Bordeaux-Bordeaux Sciences Agro, UMR 5248, 1 Cours du Général de Gaulle, 33175, Gradignan, France

    Maria-Camino Urdaci

Authors
  1. Imen Ben Slimene
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  2. Olfa Tabbene
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  3. Dorra Gharbi
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  4. Bacem Mnasri
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  6. Maria-Camino Urdaci
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  7. Ferid Limam
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Correspondence to Ferid Limam.

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Slimene, I.B., Tabbene, O., Gharbi, D. et al. Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection. Appl Biochem Biotechnol 175, 3494–3506 (2015). https://doi.org/10.1007/s12010-015-1520-7

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  • DOI: https://doi.org/10.1007/s12010-015-1520-7

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