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Production of γ-Decalactone by a Psychrophilic and a Mesophilic Strain of the Yeast Rhodotorula aurantiaca

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Abstract

Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and γ-decalactone production at low temperatures. The effects of temperature, initial pH, and castor oil concentration on the growth and γ-decalactone production by a psychrophilic and a mesophilic strain of R. aurantiaca were investigated. The highest γ-decalactone production in flasks (5.8 g/l) was obtained with the strain A19 at 14 °C and initial pH 7.0 in medium containing 20 g/l castor oil. On the other hand, these factors did not affect the production of γ-decalactone by the mesophilic strain. In fermentor, a γ-decalactone concentration of 6.6 g/l was reached with the strain A19, whereas a maximum of 0.1 g/l was obtained with the mesophilic strain. Our results suggest that the ability to synthesize γ-decalactone is a particularity of the strain A19, since the mesophilic strain (no. 30645) produced small amounts, and the other (no. 31354) did not exhibit this property. It is, to our knowledge, the first report of γ-decalactone production by R. aurantiaca and furthermore by a psychrophilic yeast strain. Moreover, the amount of γ-decalactone obtained in fermentor with the strain 19 was on the order of concentrations usually described in patents.

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References

  1. Spinnler, H. E., Dufossé, L., Souchon, I., Latrasse, A., Piffaut, C., Voilley, A., et al.(1994), French Patent No. 2705971.

  2. Endrizzi, A., Pagot, Y., Le Clainche, A., Nicaud, J. M., & Belin, J. M. (1996). Critical Reviews in Biotechnology, 16, 301–329. doi:10.3109/07388559609147424.

    Article  CAS  Google Scholar 

  3. Pagot, Y., Endrizzi, A., Nicaud, J. M., & Belin, J. M. (1997). Letters in Applied Microbiology, 25, 113–116. doi:10.1046/j.1472-765X.1997.00182.x.

    Article  CAS  Google Scholar 

  4. Wang, X. D., Mauvais, G., Cachon, R., Diviès, C., & Féron, G. (2000). Journal of Bioscience and Bioengineering, 90, 338–340.

    CAS  Google Scholar 

  5. Farbood, M.I., & Willis, B.J.(1985), US Patent 4.560.656.

  6. Lee, S. L., & Chou, C. C. (1994). Journal of Fermentation and Bioengineering, 78, 114–116. doi:10.1016/0922-338X(94)90191-0.

    Article  CAS  Google Scholar 

  7. Waché, Y., Aguedo, M., Le-Dall, M. T., Nicaud, J. M., & Belin, J. M. (2002). Journal of Molecular Catalysis. B, Enzymatic, 19–20, 347–351. doi:10.1016/S1381-1177(02)00185-6.

    Article  Google Scholar 

  8. Groguenin, A., Waché, Y., Escamilla García, E., Aguedo, M., Husson, F., Le-Dall, M. T., et al. (2004). Journal of Molecular Catalysis. B, Enzymatic, 28, 75–79. doi:10.1016/j.molcatb.2004.01.006.

    Article  CAS  Google Scholar 

  9. Aguedo, M., Gomes, N., Escamilla Garcia, E., Waché, Y., Mota, M., Teixeira, J. A., et al. (2005). Biotechnology Letters, 27, 1617–1621. doi:10.1007/s10529-005-2517-z.

    Article  CAS  Google Scholar 

  10. Escamilla García, E., Belin, J. M., & Waché, Y. (2007). Journal of Applied Microbiology, 103, 1508–1515. doi:10.1111/j.1365-2672.2007.03379.x.

    Article  Google Scholar 

  11. Gomes, N., Aguedo, M., Teixeira, J., & Belo, I. (2007). Biochemical Engineering Journal, 35, 380–386. doi:10.1016/j.bej.2007.02.002.

    Article  CAS  Google Scholar 

  12. Sarris, J., & Latrasse, A. (1985). Agricultural and Biological Chemistry, 49, 3227–3230.

    CAS  Google Scholar 

  13. Kapfer, G. F., & Berger, R. G. (1989). Biotechnology Letters, 11, 561–566. doi:10.1007/BF01040036.

    Article  CAS  Google Scholar 

  14. Féron, G., Dufossé, L., Pierard, E., Bonnarme, P., Le-Quere, J. L., & Spinnler, H. E. (1996). Applied and Environmental Microbiology, 62, 2826–2831.

    Google Scholar 

  15. Dufossé, L., Feron, G., Mauvais, G., Bonnarme, P., Durand, A., & Spinnler, H. E. (1998). Journal of Fermentation and Bioengineering, 86, 169–173. doi:10.1016/S0922-338X(98)80056-1.

    Article  Google Scholar 

  16. Lee, S. L., Cheng, H. Y., Chen, W. C., & Chou, C. C. (1999). Process Biochemistry, 34, 845–850. doi:10.1016/S0032-9592(99)00010-2.

    Article  CAS  Google Scholar 

  17. Dufossé, L., Feron, G., Latrasse, A., Guichard, E., & Spinnler, H. E. (1997). Chirality, 9, 667–671. doi:10.1002/(SICI)1520-636X(1997)9:7<667::AID-CHIR5>3.0.CO;2-4.

    Article  Google Scholar 

  18. Lee, S. L., Lin, S. J., & Chou, C. C. (1995). Journal of Fermentation and Bioengineering, 80, 195–199. doi:10.1016/0922-338X(95)93219-A.

    Article  CAS  Google Scholar 

  19. Sabri, A., Jacques, P., Weekers, F., Baré, G., Hiligsmann, S., Moussaïf, M., et al. (2000). Applied Biochemistry and Biotechnology, 84–86, 391–399. doi:10.1385/ABAB:84-86:1-9:391.

    Article  Google Scholar 

  20. Sabri, A., Baré, G., Jaques, P., Jabrane, A., Ongena, M., Van Heugen, J. C., et al. (2001). The Journal of Biological Chemistry, 276, 12691–12696. doi:10.1074/jbc.M100155200.

    Article  CAS  Google Scholar 

  21. Endrizzi, A., Awade, A. C., & Belin, J. M. (1993). FEMS Microbiology Letters, 114, 153–160.

    CAS  Google Scholar 

  22. Lee, S. L., Cheng, H. Y., Chen, W. C., & Chou, C. C. (1998). Process Biochemistry, 33, 453–459. doi:10.1016/S0032-9592(98)00013-2.

    Article  CAS  Google Scholar 

  23. Aguedo, M., Waché, Y., Coste, F., Husson, F., & Belin, J. M. (2004). Journal of Molecular Catalysis. B, Enzymatic, 29, 31–36. doi:10.1016/j.molcatb.2003.11.018.

    Article  CAS  Google Scholar 

  24. Cheetam, P. S. J., Maume, K. A., & De Rooij, J. F. M. (1993), US Patent 5. 219.742.

  25. Boog, A. L. G. M., Van Grinsven, A. M., Peters, A. L. J., Roos, R., & Wieg, A. J.(1998), US patent. 5.789.212.

  26. Rabenhorst, J., & Gatfield, I.(2002), US Patent. 6.451.565.

  27. Waché, Y., Aguedo, M., Choquet, A., Gatfield, I. L., Nicaud, J. M., & Belin, J. M. (2001). Applied and Environmental Microbiology, 67, 5700–5704. doi:10.1128/AEM.67.12.5700-5704.2001.

    Article  Google Scholar 

  28. Pagot, Y., & Belin, J. M. (1996). Applied Microbiology and Biotechnology, 45, 349–354. doi:10.1007/s002530050695.

    Article  CAS  Google Scholar 

  29. Blin-Perrin, C., Molle, D., Dufosse, L., Le-Quere, J. L., Viel, C., Mauvais, G., et al. (2000). FEMS Microbiology Letters, 188, 69–74.

    CAS  Google Scholar 

Download references

Acknowledgement

We thank the government of Syria for their financial support to carry out this study.

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Authors and Affiliations

  1. Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Mohamed Alchihab, Jacqueline Destain, Lamia Majad, Hakim Ghalfi & Philippe Thonart

  2. Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Mario Aguedo & Jean-Paul Wathelet

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  1. Mohamed Alchihab
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  2. Jacqueline Destain
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  3. Mario Aguedo
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  4. Lamia Majad
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Correspondence to Mohamed Alchihab.

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Alchihab, M., Destain, J., Aguedo, M. et al. Production of γ-Decalactone by a Psychrophilic and a Mesophilic Strain of the Yeast Rhodotorula aurantiaca . Appl Biochem Biotechnol 158, 41–50 (2009). https://doi.org/10.1007/s12010-008-8297-x

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  • DOI: https://doi.org/10.1007/s12010-008-8297-x

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