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Enhanced stability of Candida antarctica lipase B in ionic liquids

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Abstract

The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][Tf2N]. In hydrophobic ILs such as [Edmim][Tf2N], [Emim][Tf2N] and [Pmim] [PF6], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][Tf2N], [Emim][Tf2N] and [Pmim][PF6] were also well maintained after 1 day incubation at 80 °C. The lipase suspended in [Edmim][Tf2N] could be successfully reused 6 times without loss of activity.

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Authors and Affiliations

  1. ERC for Advanced Bioseparation Technology, Inha University, Incheon, 402-751, Korea

    Sung Ho Ha, Sang Hyun Lee, Woo-Jin Chang & Yoon-Mo Koo

  2. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Dung Thanh Dang, Min Sik Kwon & Yoon-Mo Koo

  3. C-TRI, Hwaseong-city, Gyeonggi-do, 445-743, Korea

    Yong Jae Yu & Il Suk Byun

Authors
  1. Sung Ho Ha
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  2. Sang Hyun Lee
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  3. Dung Thanh Dang
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  4. Min Sik Kwon
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  5. Woo-Jin Chang
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  6. Yong Jae Yu
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  7. Il Suk Byun
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  8. Yoon-Mo Koo
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Correspondence to Yoon-Mo Koo.

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Ha, S.H., Lee, S.H., Dang, D.T. et al. Enhanced stability of Candida antarctica lipase B in ionic liquids. Korean J. Chem. Eng. 25, 291–294 (2008). https://doi.org/10.1007/s11814-008-0051-0

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  • DOI: https://doi.org/10.1007/s11814-008-0051-0

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