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Flaxseed Treatments to Reduce Biohydrogenation of α-Linolenic Acid by Rumen Microbes in Cattle

  • Communication
  • Published:
Lipids

Abstract

Enrichment of beef muscle with n-3 fatty acids (FA) is one means to introduce these FA into the diet, but ruminal biohydrogenation limits their bioavailability. To address this problem, we evaluated the ability of condensed tannin (quebracho), in the presence or absence of casein, to protect 18:3n-3 in flaxseed from hydrogenation by ruminal microbes in cattle using an in vitro fermentation approach coupled with evaluation in cattle in vivo. Treated and untreated flaxseed was incubated with bovine rumen fluid for 0 and 24 h. With tannin treated flaxseed, hydrogenation of 18:3n-3 was limited to only 13% over 24 h compared to 43% for untreated flaxseed, while addition of casein to the tannin added no additional protection. To determine if a similar level of protection would occur in vivo, we used two groups of five steers fed either a grain-based or forage-based diet. Five steers were given a grain-based diet during the trial and were fed either ground flaxseed or tannin treated flaxseed for 15 days prior to blood collection for plasma lipid fatty acid analysis. The forage fed steers followed the same regimen. Ingestion of tannin-treated flaxseed did not increase 18:3n-3 and 20:5n-3 in plasma neutral lipids as compared to non-treated flaxseed. Thus, we demonstrated that treating ground flaxseed with quebracho tannin is not useful for increasing 18:3n-3 in the neutral lipid of bovine blood plasma, and suggest caution when interpreting results from in vitro trials that test potential treatments for protecting fatty acids from hydrogenation by ruminal microbes.

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References

  1. Simopoulos AP (1999) Essential FA in health and chronic diseases. Am J Clin Nutr 70(Suppl):560S–569S

    PubMed  CAS  Google Scholar 

  2. Kris-Etherton PM, Harris WS, Appel LJ (2003) Omega-3 fatty acids and cardiovascular disease: new recommendations from the American Heart Association. Arterioscler Thromb Vasc Biol 23:151–152

    Article  PubMed  CAS  Google Scholar 

  3. Economic Research Service, Fishery Products—Per Capita Consumption. http://www.ers.usda.gov/Data/FoodConsumption/spreadsheets/mtfish.xls. Accessed Dec 2006

  4. Myers RA, Worm B (2003) Rapid worldwide depletion of predatory fish communities. Nature 423:280–283

    Article  PubMed  CAS  Google Scholar 

  5. Naylor R, Burke M (2005) Aquaculture and ocean resources: raising tigers of the sea. Annu Rev Environ Resour 30:185–218

    Article  Google Scholar 

  6. Economic Research Service, Total Red Meat—Per Capita Consumption. http://www.ers.usda.gov/Data/FoodConsumption/spreadsheets/mtredsu.xls#total!a1. Accessed Dec 2006

  7. Rule DC, Broughton KS, Shellito SM, Maiorano G (2002) Comparison of muscle fatty acid profiles and cholesterol concentrations of bison, beef cattle, elk, and chicken. J Anim Sci 80:1202–1211

    PubMed  CAS  Google Scholar 

  8. Scollan ND, Dhanoa MS, Choi NJ, Maeng WJ, Enser M, Wood JD (2001) Biohydrogenation and digestion of long chain fatty acids in steers fed on different sources of lipid. J Agric Sci 136:345–355

    Article  CAS  Google Scholar 

  9. Kronberg SL, Barceló-Coblijn G, Shin J, Lee K, Murphy EJ (2006) Bovine muscle n-3 fatty acid content is increased with flaxseed feeding. Lipids 41:1059–1069

    Article  PubMed  CAS  Google Scholar 

  10. Maddock TD, Bauer ML, Koch KB, Anderson VL, Maddock RJ, Barceló-Coblijn G, Murphy EJ, Lardy GP (2006) Effect of processing flax in beef feedlot diets on performance, carcass characteristics, and trained sensory panel ratings. J Anim Sci 84:1544–1551

    PubMed  CAS  Google Scholar 

  11. Moore JH, Noble RC, Steele W (1969) The incorporation of linolenic and linoleic acids into the plasma lipids of sheep given intra-abomasal infusions of linseed oil, maize oil or linoleic acid. Br J Nutr 23:141–152

    Article  PubMed  CAS  Google Scholar 

  12. Scott TW, Cook LJ, Mills SC (1971) Protection of dietary polyunsaturated fatty acids against microbial hydrogenation in ruminants. J Amer Oil Chem Soc 48:358–364

    Article  CAS  Google Scholar 

  13. Scislowski V, Bauchart D, Gruffat D, Laplaud PM, Durand D (2005) Effects of dietary n-6 or n-3 polyunsaturated fatty acids protected or not protected against ruminal hydrogenation on plasma lipids and their susceptibility to peroxidation in fattening steers. J Anim Sci 83:2162–2174

    PubMed  CAS  Google Scholar 

  14. Ogilvie BM, McClymont GL (1961) Effect of duodenal administration of highly unsaturated fatty acids on composition of ruminant depot fat. Nature 190:725–726

    Article  PubMed  CAS  Google Scholar 

  15. Scott TW, Ashes JR (1993) Dietary lipids for ruminants: protection, utilization and effects on remodelling of skeletal muscle phospholipids. Aust J Agric Res 44:495–508

    Article  CAS  Google Scholar 

  16. Scollan ND, Enser M, Gulati SK, Richardson I, Wood JD (2003) Effects of including a ruminally protected lipid supplement in the diet on the fatty acid composition of beef muscle. Brit J Nutr 90:709–716

    Article  PubMed  CAS  Google Scholar 

  17. Ashes JR, Siebert BD, Gulati SK, Cuthbertson AZ, Scott TW (1992) Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27:629–631

    Article  PubMed  CAS  Google Scholar 

  18. Ashes JR, Gulati SK, Cook LJ, Scott TW, Donnelly JB (1979) Assessing the biological effectiveness of protected lipid supplements for ruminants. J Am Oil Chem Soc 56:522–527

    Article  CAS  Google Scholar 

  19. Petit HV, Dewhurst RJ, Scollan ND, Prouix JG, Khalid M, Haresign W, Twagiramungu H, Mann GE (2002) Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. J Dairy Sci 85:889–899

    PubMed  CAS  Google Scholar 

  20. Sinclair LA, Cooper SL, Huntington JA, Wilkinson RG, Hallet KG, Enser M, Wood JD (2005) In vitro biohydrogenation of n-3 polyunsaturated fatty acids protected against ruminal microbial metabolism. Anim Feed Sci Tech 123–124:579–596

    Article  CAS  Google Scholar 

  21. Van Nevel C, Demeyer DI (1995) Lipolysis and biohydrogenation of soybean oil in the rumen in vitro: inhibition by antimicrobials. J Dairy Sci 78:2797–2806

    Article  PubMed  Google Scholar 

  22. Van Nevel CJ, Demeyer DI (1996) Effect of pH on biohydrogenation of polyunsaturated fatty acids and their Ca-salts by rumen microorganisms in vitro. Arch Anim Nutr 49:151–157

    Article  Google Scholar 

  23. Petit HV, Tremblay GF, Tremblay E, Nadeau P (2002) Ruminal biohydrogenation of fatty acids, protein degradability, and dry matter digestibility of flaxseed treated with different sugar and heat combinations. Can J Anim Sci 82:241–250

    CAS  Google Scholar 

  24. Gonthier C, Mustafa AF, Berthiaume R, Petit HV, Martineau R, Ouellet DR (2004) Effects of feeding micronized and extruded flaxseed on ruminal fermentation and nutrient utilization by dairy cows. J Dairy Sci 87:1854–1863

    PubMed  CAS  Google Scholar 

  25. Heguy JM, Juchem SO, DePeters EJ, Rosenberg M, Santos JEP, Taylor SJ (2006) Whey protein gel composites of soybean and linseed oils as a dietary method to modify the unsaturated fatty acid composition of milk lipids. Anim Feed Sci Tech 131:370–388

    Article  CAS  Google Scholar 

  26. Carroll SM, DePeters EJ, Rosenberg M (2006) Efficacy of a novel whey protein gel complex to increase the unsaturated fatty acid composition of bovine milk fat. J Dairy Sci 89:640–650

    PubMed  CAS  Google Scholar 

  27. Jones GA, McAllister TA, Muir AD, Cheng J (1994) Effects of sainfoin (Onobrychis viciifolia Scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Appl Environ Microbiol 60:1374–1378

    PubMed  CAS  Google Scholar 

  28. McSweeney CS, Palmer B, McNeill DM, Krause DO (2001) Microbial interactions with tannins: nutritional consequences for ruminants. Anim Feed Sci Tech 91:83–93

    Article  CAS  Google Scholar 

  29. SAS (1996) SAS systems for mixed models. SAS Institute, Inc., Cary

    Google Scholar 

  30. NRC (2000) Nutrient requirements of beef cattle. 7th edn. Natl Acad Press, Washington

    Google Scholar 

  31. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    PubMed  CAS  Google Scholar 

  32. Brockerhoff H (1975) Determination of the positional distribution of fatty acids in glycerolipids. Meth Enzymol 35:315–325

    PubMed  CAS  Google Scholar 

  33. Whitney MB, Hess BW, Kaltenbach JE, Harlow HJ, Rule DC (1999) Direct transesterification of lipids from feedstuffs and ruminal bacteria. Can J Anim Sci 79:247–249

    Article  Google Scholar 

  34. Kucuk O, Hess BW, Ludden PA, Rule DC (2001) Effect of forage:concentrate ratio on ruminal digestion and duodenal flow of fatty acids in ewes. J Anim Sci 79:2233–2240

    PubMed  CAS  Google Scholar 

  35. Lake SL, Scholljegerdes EJ, Weston TR, Rule DC, Hess BW (2006) Postpartum supplemental fat, but not maternal body condition score at parturition, affects plasma and adipose tissue fatty acid profiles of suckling beef calves. J Anim Sci 84:811–1819

    Article  CAS  Google Scholar 

  36. Waghorn GC, Ulyatt MJ, John A, Fisher MT (1987) The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed Lotus corniculatus L. Brit J Nutr 57:115–126

    Article  PubMed  CAS  Google Scholar 

  37. Gulati SK, Ashes JR, Scott TW (1999) Hydrogenation of eicosapentaenoic and docosahexaenoic acids and their incorporation into milk fat. Anim Feed Sci Tech 79:57–64

    Article  CAS  Google Scholar 

  38. Jouany JP, Lassalas B, Doreau M, Glasser F (2007) Dynamic features of the rumen metabolism of linoleic acid, linolenic acid and linseed oil measured in vitro. Lipids 42:351–360

    Article  PubMed  CAS  Google Scholar 

  39. Aerts RJ, McNabb WC, Molan A, Brand A, Barry TN, Peters JS (1999) Condensed tannin from Lotus corniculatus and Lotus pedunculatus exert different effects on the in vitro rumen degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) protein. J Sci Food Agric 79:79–85

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Clay Erickson and Curtis Klein for assistance with cattle management and feeding, and Faye Kroh and Becky Wald for feed nutritional analysis and assistance with fatty acid analysis of feed and fermentation residue. This work was supported, in part, by the intramural research program of the ARA, by the Healthy Beef Initiative, and by NIH grant #1P20 RR17699 (EJM).

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Author notes

  1. G. Barceló-Coblijn

    Present address: Laboratori de Biomedicina Molecular i Cellular, Departament de Biología, Facultat de Ciències, Universitat de les Illes Balears, Cra. de Valldemossa, km 7.5, E-07122, Palma, Illes Balears, Spain 

Authors and Affiliations

  1. USDA, Agricultural Research Service, Northern Great Plains Research Laboratory, P.O. Box 459, Mandan, ND, 58554, USA

    S. L. Kronberg & E. J. Scholljegerdes

  2. Department of Pharmacology, Physiology and Therapeutics, University of North Dakota, Grand Forks, ND, 58202-9037, USA

    G. Barceló-Coblijn & E. J. Murphy

  3. Department of Chemistry, University of North Dakota, Grand Forks, ND, 58202-9037, USA

    E. J. Murphy

Authors
  1. S. L. Kronberg
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  2. E. J. Scholljegerdes
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  3. G. Barceló-Coblijn
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  4. E. J. Murphy
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Correspondence to S. L. Kronberg.

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Kronberg, S.L., Scholljegerdes, E.J., Barceló-Coblijn, G. et al. Flaxseed Treatments to Reduce Biohydrogenation of α-Linolenic Acid by Rumen Microbes in Cattle. Lipids 42, 1105–1111 (2007). https://doi.org/10.1007/s11745-007-3126-5

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  • DOI: https://doi.org/10.1007/s11745-007-3126-5

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