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Purification and Characterization of Fungal Poly(3-hydroxybutyrate) Depolymerase from Paecilomyces lilacinus F4-5 and Enzymatic Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Film

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Summary

Poly(3-hydroxybutyrate) [P(3HB)] depolymerase was purified from a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]-degrading fungus, Paecilomyces lilacinus F4-5 by hydrophobic and ion exchange column chromatography, and showed a molecular mass of 45 kDa. The optimum temperature and pH of the P(3HB) depolymerase were 50 °C and 7.0, respectively. The enzyme was stable for at least 30 min at temperatures below 40 °C, while the activity abruptly decreased over 55 °C. Enzymatic P(3HB-co-3HV) degradation showed a similar degradation pattern to that of film overlaid by fungal hyphae. It reflects that the fungal degradation of P(3HB-co-3HV) in soil is mainly caused by extracellular depolymerases.

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Authors and Affiliations

  1. Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, 136-791, Seongbuk-Ku, Seoul, Korea

    Byoung-In Sang & Won-Kwon Lee

  2. Department of Biotechnology, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, 226-8501, Midori, Yokohama, Japan

    Katsutoshi Hori & Hajime Unno

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  1. Byoung-In Sang
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  2. Won-Kwon Lee
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  3. Katsutoshi Hori
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  4. Hajime Unno
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Correspondence to Byoung-In Sang.

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Sang, BI., Lee, WK., Hori, K. et al. Purification and Characterization of Fungal Poly(3-hydroxybutyrate) Depolymerase from Paecilomyces lilacinus F4-5 and Enzymatic Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Film. World J Microbiol Biotechnol 22, 51–57 (2006). https://doi.org/10.1007/s11274-005-5773-0

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  • DOI: https://doi.org/10.1007/s11274-005-5773-0

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