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Continuous citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N ratio

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Summary

The central aspect of this work was to investigate the influence of nitrogen feed rate at constant C/N ratio on continuous citric acid fermentation by Candida oleophila ATCC 20177. Medium ammonia nitrogen and glucose concentrations influenced growth and production. Space-time yield (STY) meaning volumetric productivity, biomass specific productivity (BSP), product concentration, product selectivity and citrate/isocitrate ratio increased with increasing residence time (RT). BSP increased in an exponential mode lowering nitrogen feed rates. Highest BSP for citric acid of 0.13 g/(g h) was achieved at lowest NH4Cl concentration of 1.5 g/l and highest STY (1.2 g/l h) with 3 g NH4Cl/l at a RT of 25 h. Citric acid 74.2 g/l were produced at 58 h RT and 6 g NH4Cl/l. Glucose uptake rate seems to be strictly controlled by growth rate of the yeast cells. Optimum nitrogen concentration and adapted C/N ratio are essential for successful continuous citric acid fermentation. The biomass-specific nitrogen feed rate is the most important factor influencing continuous citric acid production by yeasts. Numerous chemostat experiments showed the feasibility of continuous citrate production by yeasts.

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Author notes

  1. S. Anastassiadis

    Present address: Research in Biotechnology, Co., Vat. #: 108851559, Avgi/Sohos, 57002, Thessaloniki, Greece

Authors and Affiliations

  1. Department of Environmental Engineering, School of Engineering, Democritus University of Thrace, 67100, Xanthi, Greece

    S. Anastassiadis

  2. Institute of Biotechnology 2, Research Centre Jülich (RCJ), D-52425, Jülich, Germany

    C. Wandrey

  3. Institute of Microbiology, University of Münster, Corrensstr. 3, 48149, Münster, Germany (retired)

    H. -J. Rehm

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  1. S. Anastassiadis
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  3. H. -J. Rehm
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Correspondence to S. Anastassiadis.

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Anastassiadis, S., Wandrey, C. & Rehm, H.J. Continuous citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N ratio. World J Microbiol Biotechnol 21, 695–705 (2005). https://doi.org/10.1007/s11274-004-3850-4

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