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Position independent and copy-number-related expression of the bovine neonatal Fc receptor α-chain in transgenic mice carrying a 102 kb BAC genomic fragment

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Abstract

We generated and characterized transgenic mice carrying a 102 kb bovine genomic fragment, encoding the neonatal Fc receptor α-chain (bFcRn). FcRn plays a crucial role in the maternal IgG transport and it also regulates the IgG and albumin homeostasis. Some of its functions and transcriptional regulation show species specific differences. The FcRn heterodimer is composed of the α-chain and beta-2-microglobulin (β2 m). A bacterial artificial chromosome containing the bovine FcRn α-chain gene (bFCGRT) with its 44 kb 5′ and 50 kb long 3′ flanking sequences was microinjected into fertilized mouse oocytes. Two of the transgenic lines generated, showed copy number related and integration site independent bFcRn expression. The bFcRn α-chain forms a functional receptor with the mouse β2-microglobulin and extends the half-life of the mouse IgG in transgenic mice. Our results underline the feasibility of creating BAC transgenic mouse models of economically important bovine genes.

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Abbreviations

FcRn:

neonatal Fc receptor

FCGRT:

gene of the FcRn α-chain

β2 m:

beta-2-microglobulin

IgG:

immunoglobulin G

BAC:

bacterial artificial chromosome

wt:

wild type

tg:

transgenic

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Acknowledgements

This work was supported by the Grants OTKA T049015, OMFB 1605-1606/2002, the Swedish Research Council and by ESF-COST B20 STSMs to Zs.B. and L.B. WinNonlin software was generously provided through an Academic License by Pharsight Corporation.

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Authors and Affiliations

  1. Agricultural Biotechnology Center, 2100, Szent-Györgyi A. st.4, Godollo, Hungary

    Balázs Bender, Lilla Bodrogi & Zsuzsanna Bősze

  2. Faculty of Veterinary Science, Szent István University, Budapest, Hungary

    Balázs Mayer & Zita Schneider

  3. Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden

    Yaofeng Zhao & Lennart Hammarström

  4. INRA, UR339, Laboratoire de Génétique biochimique et de Cytogénétique, Jouy-en-Josas, France

    André Eggen

  5. Department of Immunology, Faculty of Science, Eötvös Lóránd University, Budapest, Hungary

    Imre Kacskovics

  6. Immunology Research Group of the Hungarian Academy of Sciences at Eötvös Loránd University, Budapest, Hungary

    Imre Kacskovics

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  1. Balázs Bender
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  2. Lilla Bodrogi
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Correspondence to Zsuzsanna Bősze.

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Bender, B., Bodrogi, L., Mayer, B. et al. Position independent and copy-number-related expression of the bovine neonatal Fc receptor α-chain in transgenic mice carrying a 102 kb BAC genomic fragment. Transgenic Res 16, 613–627 (2007). https://doi.org/10.1007/s11248-007-9108-9

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  • DOI: https://doi.org/10.1007/s11248-007-9108-9

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