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The different expression of immune-related cytokine genes in response to velogenic and lentogenic Newcastle disease viruses infection in chicken peripheral blood

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Abstract

Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Peripheral blood serves as an important producer and carrier of viruses and cytokines in NDV infection. In order to explore the difference of cytokine expression in the peripheral blood between velogenic strain and lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect specific-pathogen-free (SPF) chickens separately, and peripheral blood was collected on 0, 3, 7, 10, 14, and 21 days post-infection (d.p.i.). Real-time PCR was then used to detect the expression of six kinds of immune-related cytokine genes. For the F48E9 group, a sharp increase of the expression of interferon-alpha (IFN-α), interferon-gamma (IFN-γ), interleukin-16 and IL-18 was observed on 3 d.p.i. before the NDV blood peak (7 d.p.i.), followed by a rapid decline to the level lower than that of control group, then the expression of IFN-α increased slowly and reached or exceeded the level of control group in the later phase of the infection, while the expression of IFN-γ, IL-16, and IL-18 fluctuated at the level of control group for the rest of study period. The increase of IL-2 expression was not obvious, and no increase of IL-15 expression was noted. For the Lasota (vaccine) group, the picture was quite different, a sharp increase of IFN-γ (but not IFN-α), IL-2 was observed on 7 d.p.i. before the NDV blood peak (10 d.p.i.). On the contrary, there was no dramatic increase of IL-16 and IL-18. Interestingly, in contrast to the F48E9 group, there was an increase of IL-15 on day 10 d.p.i., but it remained modest. There was also an increase of IFN-α on day 21 d.p.i. Our results revealed that infection with NDV strains of different virulence was associated with distinct cytokine expression patterns in peripheral blood, modulation of cytokine responses may play a key role in mediation of NDV pathogenesis.

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Acknowledgments

The study was financially supported by Sichuan Youth Science and Technology Foundation (Grant number: 2007Q14-034) and China Postdoctoral Science Foundation (Grant number: 2005037805) and a postdoctoral fund from Sichuan Agricultural University, as well as Program for Changjiang Scholars and Innovative Research Team in University “PCSIRT” (Grant No: IRTO848).

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Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, 46# Xinkang Road, Ya’an, 625014, Sichuan, China

    Wen-Quan Liu, Ming-Xing Tian, Yuan-Ping Wang, Yang Zhao, Nian-Li Zou, Fang-Fang Zhao, San-Jie Cao, Xin-Tian Wen, Ping Liu & Yong Huang

  2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China

    San-Jie Cao, Xin-Tian Wen, Ping Liu & Yong Huang

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  1. Wen-Quan Liu
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  2. Ming-Xing Tian
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Correspondence to Yong Huang.

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Liu, WQ., Tian, MX., Wang, YP. et al. The different expression of immune-related cytokine genes in response to velogenic and lentogenic Newcastle disease viruses infection in chicken peripheral blood. Mol Biol Rep 39, 3611–3618 (2012). https://doi.org/10.1007/s11033-011-1135-1

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  • DOI: https://doi.org/10.1007/s11033-011-1135-1

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