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Characterization of a novel male sterile mutant of Tagetes patula induced by heat shock

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Abstract

Tagetes patula L. is an annual plant belonging to the Asteraceae family. Currently, commercial F1 cultivars are produced through the use of artificial emasculation which is a very laborious procedure, particularly in view of the composite capitulum structure. To date, no male sterile line is available in this species, but the isolation of such material would provide a valuable tool for the establishment of an efficient cross-pollination system for the production of F1 hybrids. In this study, we report the characterization of a novel pollen abortion mutant of Tagetes patula which was isolated from 1,796 heat shock induced cutting seedlings. Histological and cytological observation demonstrated that the abnormal development of the microspores during the binucleate pollen stage led to pollen abortion, and this was correlated to premature tapetum degeneration. Cross hybridization experiments indicated that a recessive nuclear gene was responsible for the trait. This novel male sterile mutant of Tagetes patula appears to be an applicable material for improving the breeding efficiency of the species and may be used to significantly improve ornamental traits in F1 generations.

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Acknowledgments

This research was supported by grants from National Natural Science Foundation of China (31201647) and the Fundamental Research Funds for the Central Universities (2013PY081). We thank all past and present colleagues in our lab for constructive discussion and technical support and Dr. Alex McCormac (Mambo-Tox Ltd., UK) for critical editing of the manuscript. Ye Ai and Yanhong He contributed equally to this work.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Ye Ai, Yanhong He, Chen Pan & Manzhu Bao

  2. Europe-America Animal and Plant Research, BGI, Shenzhen, 518083, People’s Republic of China

    Yan Hu

  3. The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Qinghua Zhang

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  1. Ye Ai
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  2. Yanhong He
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  3. Yan Hu
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  4. Qinghua Zhang
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  5. Chen Pan
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  6. Manzhu Bao
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Correspondence to Manzhu Bao.

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Ye Ai and Yanhong He contributed equally to this work.

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Ai, Y., He, Y., Hu, Y. et al. Characterization of a novel male sterile mutant of Tagetes patula induced by heat shock. Euphytica 200, 159–173 (2014). https://doi.org/10.1007/s10681-014-1116-2

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