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Curium(III) complexation with pyoverdins secreted by a groundwater strain of Pseudomonas fluorescens

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Abstract

Pyoverdins, bacterial siderophores produced by ubiquitous fluorescent Pseudomonas species, have great potential to bind and thus transport actinides in the environment. Therefore, the influence of pyoverdins secreted by microbes on the migration processes of actinides must be taken into account in strategies for the risk assessment of potential nuclear waste disposal sites. The unknown interaction between curium(III) and the pyoverdins released by Pseudomonas fluorescens (CCUG 32456) isolated from the granitic rock aquifers at the Äspö Hard Rock Laboratory (Äspö HRL), Sweden, is the subject of this paper. The interaction between soluble species of curium(III) and pyoverdins was studied at trace curium(III) concentrations (3 × 10−7 M) using time-resolved laser-induced fluorescence spectroscopy (TRLFS). Three Cm3+—P. fluorescens (CCUG 32456) pyoverdin species, MpHqLr, could be identified from the fluorescence emission spectra, CmH2L+, CmHL, and CmL−, having peak maxima at 601, 607, and 611 nm, respectively. The large formation constants, log β121 = 32.50 ± 0.06, log β111 = 27.40 ± 0.11, and log β101 = 19.30 ± 0.17, compared to those of other chelating agents illustrate the unique complexation properties of pyoverdin-type siderophores. An indirect excitation mechanism for the curium(III) fluorescence was observed in the presence of the pyoverdin molecules.

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Acknowledgements

This work was funded by BMWi under contract number 02E9985. The authors are indebted to the U.S. Department of Energy, Office of Basic Energy Sciences, for the use of 248Cm via the transplutonium element production facilities at Oak Ridge National Laboratory; 248Cm was made available as part of collaboration between FZD and the Lawrence Berkeley National Laboratory (LBNL).

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Authors and Affiliations

  1. Institute of Radiochemistry, Forschungszentrum Dresden-Rossendorf, P.O. Box 510119, Dresden, 01314, Germany

    Henry Moll & Gert Bernhard

  2. Department of Cell and Molecular Biology, Microbiology, Göteborg University, P.O. Box 462, Göteborg, 405 30, Sweden

    Anna Johnsson & Karsten Pedersen

  3. Institut für Organische Chemie, Universität zu Köln, Greinstraße 4, Koln, 50939, Germany

    Mathias Schäfer & Herbert Budzikiewicz

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  1. Henry Moll
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  2. Anna Johnsson
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  3. Mathias Schäfer
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  4. Karsten Pedersen
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  5. Herbert Budzikiewicz
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  6. Gert Bernhard
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Correspondence to Henry Moll.

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Moll, H., Johnsson, A., Schäfer, M. et al. Curium(III) complexation with pyoverdins secreted by a groundwater strain of Pseudomonas fluorescens . Biometals 21, 219–228 (2008). https://doi.org/10.1007/s10534-007-9111-x

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  • DOI: https://doi.org/10.1007/s10534-007-9111-x

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