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Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins

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Abstract

Purpose

The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.

Method

Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.

Result

PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).

Conclusion

This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.

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Abbreviations

ACC:

Acetyl-coenzyme A carboxylase

AMPK:

5′ AMP-activated protein kinase

ANOVA:

One-way analyses of variance

BMI:

Body mass index

C:

Control

CS:

Citrate synthase

DXA:

Dual-energy X-ray absorptiometry

HAD:

β-Hydroxy-acyl-CoA-dehydrogenase

HSL:

Hormone-sensitive lipase

IPAQ:

International Physical Activity Questionnaire

LB:

Lower body

MFO:

Maximal fat oxidation

O:

Obese

OGTT:

Oral glucose tolerance test

PDH:

Pyruvate dehydrogenase

PDK4:

Pyruvate dehydrogenase kinase-4

PGC-1alpha:

Peroxisome proliferator-activated receptor-γ coactivator-1α

PO:

Postobese

Ser:

Serine

Sir2:

Silent information regulator 2

SIRT1:

Sirtuin 1

SIRT3:

Sirtuin 3

TCA:

Tricarboxylic acid cycle or the Krebs cycle

Thr:

Threonine

UB:

Upper body

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Acknowledgments

This study was supported by grants from the Ministerio de Educación y Ciencia (DEP2010-21866, and FEDER), FUNCIS (PI/10/07), Programa Innova Canarias 2020 (P.PE03-01-F08), Proyecto Estructurante de la ULPGC: ULPAPD-08/01-4, and Proyecto del Programa Propio de la ULPGC (ULPGC 2009-07), and III Convocatoria de Ayudas a la Investigación Cátedra Real Madrid- UEM (2010/01RM, Universidad Europea de Madrid). Financial support was received from the Novo Nordisk Foundation, The Foundation of 1870 and Christian d. 10 foundation. Furthermore, we thank Jesper Isager Sally, Thomas Beck, Jeppe Bach and Regitze Kraunsøe (all Dept. Biomedical Sciences, University of Copenhagen) for providing excellent technical assistance. In addition, we thank Prof. Bente Stallknecht (Dept. Biomedical Sciences, University of Copenhagen) for performing the biopsies. Special thanks are given to José Navarro de Tuero, and María Carmen García Chicano for their excellent technical assistance.

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Authors and Affiliations

  1. Department of Physical Education, University of Las Palmas de Gran Canaria, Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria, Canary Islands, Las Palmas, Spain

    Jesús Gustavo Ponce-González, Borja Guerra & Jose A. L. Calbet

  2. GENUD Toledo Research Group, University of Castilla-La Mancha, Toledo, Spain

    Ignacio Ara

  3. Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark

    Ignacio Ara, Steen Larsen & Jørn Wulff Helge

  4. Research Institute of Biomedical and Health Sciences (IUIBS), Las Palmas de Gran Canaria, Canary Islands, Spain

    Borja Guerra & Jose A. L. Calbet

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  1. Jesús Gustavo Ponce-González
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  2. Ignacio Ara
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Correspondence to Jesús Gustavo Ponce-González.

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Communicated by Anni Vanhatalo.

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Ponce-González, J.G., Ara, I., Larsen, S. et al. Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins. Eur J Appl Physiol 116, 11–18 (2016). https://doi.org/10.1007/s00421-015-3232-7

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