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Apparent diffusion coefficient correlation with oesophageal tumour stroma and angiogenesis

  • Gastrointestinal
  • Published:
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Abstract

Objective

Because diffusion-weighted imaging (DWI) can predict the prognosis of patients with oesophageal squamous cell carcinoma (ESCC), we hypothesised that apparent diffusion coefficient (ADC) values might be correlated with the collagen content and tumour angiogenesis. The purpose of this study was to determine the correlation between ADC values of ESCC before treatment and oesophageal tumour stroma and angiogenesis.

Methods

Seventeen patients with ESCC were enrolled. The ADC values were calculated from the DWI score. Seventeen patients who had undergone oesophagectomy were analysed for tumour stroma, vascular endothelial growth factor (VEGF) and CD34. Tissue collagen was stained with azocarmine and aniline blue to quantitatively analyse the extracellular matrix in cancer stroma. Tissues were stained with VEGF and CD34 to analyse the angiogenesis.

Results

The ADC values decreased with stromal collagen growth. We found a negative correlation between the tumour ADC and the amount of stromal collagen (r = −0.729, P = 0.001), i.e. the ADC values decreased with growth of VEGF. We also found a negative correlation between the ADC of the tumours and the amount of VEGF (r = 0.538, P = 0.026).

Conclusion

Our results indicated that the ADC value may be a novel prognostic factor and contribute to the treatment of oesophageal cancer.

Key Points

• Magnetic resonance apparent diffusion coefficient values inversely indicate tumour stromal collagen

• There is also negative correlation between ADCs and vascular endothelial growth factor

• ADC values may contribute to the treatment of oesophageal cancer.

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References

  1. Aoyagi T, Shuto K, Okazumi S et al (2010) Evaluation of the clinical staging of esophageal cancer by using diffusion-weighted imaging. Exp Ther Med 1(5):847–852

    Google Scholar 

  2. Aoyagi T, Shuto K, Okazumi S et al (2010) Evaluation of ulcerative colitis using diffusion-weighted imaging. Hepatogastroenterology 57(99–100):468–471

    PubMed  Google Scholar 

  3. Aoyagi T, Shuto K, Okazumi S et al (2011) Apparent diffusion coefficient values measured by diffusion-weighted imaging predict chemoradiotherapeutic effect for advanced esophageal cancer. Dig Surg 28:252–257

    Article  PubMed  Google Scholar 

  4. Matoba M, Tonami H, Kondou T et al (2007) Lung carcinoma: diffusion-weighted MR imaging-preliminary evaluation with apparent diffusion coefficient. Radiology 243:570–577

    Article  PubMed  Google Scholar 

  5. Baba Y, Iyama K, Honda S, Ishikawa S, Miyanari N, Baba H (2006) Cytoplasmic expression of type VII collagen is related to prognosis in patients with esophageal squamous cell carcinoma. Oncology 71:221–228

    Article  PubMed  CAS  Google Scholar 

  6. Baba Y, Iyama K, Ikeda K et al (2008) The expression of type IV collagen alpha6 chain is related to the prognosis in patients with esophageal squamous cell carcinoma. Ann Surg Oncol 15:555–565

    Article  PubMed  Google Scholar 

  7. Nabeya Y, Shimada H, Okazumi S et al (2002) Serum cross-linked carboxyterminal telopeptide of type I collagen (ICTP) as a prognostic tumor marker in patients with esophageal squamous cell carcinoma. Cancer 94:940–949

    Article  PubMed  CAS  Google Scholar 

  8. Takahara T, Imai Y, Yamashita T, Yasuda S, Nasu S, Van Cauteren M (2004) Diffusion weighted whole body imaging with background body signal suppression (DWIBS): technical improvement using free breathing, STIR and high resolution 3D display. Radiat Med 22:275–282

    PubMed  Google Scholar 

  9. Sobin LHW, Wittekind Ch (2002) TNM: Classification of malignant tumours, 6th edn. Wiley-Liss, New York

    Google Scholar 

  10. Ohno S, Tachibana M, Fujii T, Ueda S, Kubota H, Nagasue N (2002) Role of stromal collagen in immunomodulation and prognosis of advanced gastric carcinoma. Int J Cancer 97:770–774

    Article  PubMed  CAS  Google Scholar 

  11. Bhowmick NA, Neilson EG, Moses HL (2004) Stromal fibroblasts in cancer initiation and progression. Nature 432:332–337

    Article  PubMed  CAS  Google Scholar 

  12. Liotta LA, Kohn EC (2001) The microenvironment of the tumour-host interface. Nature 411:375–379

    Article  PubMed  CAS  Google Scholar 

  13. Heldin CH, Rubin K, Pietras K, Ostman A (2004) High interstitial fluid pressure —an obstacle in cancer therapy. Nat Rev 4:806–813

    Article  CAS  Google Scholar 

  14. Netti PA, Berk DA, Swartz MA, Grodzinsky AJ, Jain RK (2000) Role of extracellular matrix assembly in interstitial transport in solid tumors. Cancer Res 60:2497–2503

    PubMed  CAS  Google Scholar 

  15. Oldberg A, Kalamajski S, Salnikov AV et al (2007) Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure and fluid balance in experimental carcinoma. Proc Natl Acad Sci USA 104:13966–13971

    Article  PubMed  CAS  Google Scholar 

  16. Boucher Y, Baxter LT, Jain RK (1990) Interstitial pressure gradients in tissue-isolated and subcutaneous tumors: implications for therapy. Cancer Res 50:4478–4484

    PubMed  CAS  Google Scholar 

  17. Stohrer M, Boucher Y, Stangassinger M, Jain RK (2000) Oncotic pressure in solid tumors is elevated. Cancer Res 60:4251–4255

    PubMed  CAS  Google Scholar 

  18. Nordsmark M, Overgaard M, Overgaard J (1996) Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol 41:31–39

    PubMed  CAS  Google Scholar 

  19. Janssen HL, Haustermans KM, Balm AJ, Begg AC (2005) Hypoxia in head and neck cancer: how much, how important? Head Neck 27:622–638

    Article  PubMed  CAS  Google Scholar 

  20. Inoue K, Ozeki Y, Suganuma T (1997) Vascular endothelial growth factor expression in primary esophageal squamous cell carcinoma. Association with angiogenesis and tumor progression. Cancer 79:206–213

    Article  PubMed  CAS  Google Scholar 

  21. Kulle MH, Odze RD, Mueller JD et al (2004) Prognostic significance of vascular endothelial growth factor and cyclooxygenase 2 expression in patients receiving preoperative chemoradiation for esophageal cancer. J Thorac Cardiovasc Surg 127:1579–1586

    Article  Google Scholar 

  22. Imdahl A, Bognar G, Schulte-Monting J et al (2002) Predictive factors for response to neoadjuvant therapy in patients with oesophageal cancer. Eur J Cardiothorac Surg 21:657–663

    Article  PubMed  CAS  Google Scholar 

  23. Shimada H, Hoshino T, Okazumi S et al (2002) Expression of angiogenic factors predicts response to chemoradiotherapy and prognosis of oesophageal squamous cell carcinoma. Br J Cancer 86:552–557

    Article  PubMed  CAS  Google Scholar 

  24. Kleespies A, Guba M, Jauch KW, Bruns CJ (2004) Vascular endothelial growth factor in esophageal cancer. J Surg Oncol 87:95–104

    Article  PubMed  CAS  Google Scholar 

  25. Shih CH, Ozawa S, Ando N, Ueda M, Kitajima M (2000) Vascular endothelial growth factor expression predicts outcome and lymph node metastasis in squamous cell carcinoma of the esophagus. Clin Cancer Res 6:1161–1168

    PubMed  CAS  Google Scholar 

  26. Shimada H, Takeda A, Nabeya Y et al (2001) Clinical significance of serum vascular endothelial growth factor in esophageal squamous cell carcinoma. Cancer 92:663–669

    Article  PubMed  CAS  Google Scholar 

  27. Ogata Y, Fujita H, Yamana H, Sueyoshi S, Shirouzu K (2003) Expression of vascular endothelial growth factor as a prognostic factor in node-positive squamous cell carcinoma in the thoracic esophagus: long-term follow-up study. World J Surg 27:584–589

    Article  PubMed  Google Scholar 

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The authors have no financial or proprietary interests in any aspect of this report.

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Authors and Affiliations

  1. Department of Frontier Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi, Chiba, 260-8670, Japan

    Tomoyoshi Aoyagi, Kiyohiko Shuto, Shinichi Okazumi, Kohichi Hayano, Asami Satoh, Hiroshige Saitoh, Hideaki Shimada, Yoshihiro Nabeya & Hisahiro Matsubara

  2. Department of Radiology, Graduate School of Medicine, Chiba University, Chiba, Japan

    Toshiki Kazama

Authors
  1. Tomoyoshi Aoyagi
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  2. Kiyohiko Shuto
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  3. Shinichi Okazumi
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  4. Kohichi Hayano
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  5. Asami Satoh
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  6. Hiroshige Saitoh
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  7. Hideaki Shimada
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  8. Yoshihiro Nabeya
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  9. Toshiki Kazama
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  10. Hisahiro Matsubara
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Corresponding author

Correspondence to Tomoyoshi Aoyagi.

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Aoyagi, T., Shuto, K., Okazumi, S. et al. Apparent diffusion coefficient correlation with oesophageal tumour stroma and angiogenesis. Eur Radiol 22, 1172–1177 (2012). https://doi.org/10.1007/s00330-011-2359-0

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  • DOI: https://doi.org/10.1007/s00330-011-2359-0

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