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Genetic mapping of the Leptosphaeria maculans avirulence gene corresponding to the LepR1 resistance gene of Brassica napus

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Abstract

AvrLepR1 of the fungal pathogen Leptosphaeria maculans is the avirulence gene that corresponds to Brassica LepR1, a plant gene controlling dominant, race-specific resistance to this pathogen. An in vitro cross between the virulent L. maculans isolate, 87-41, and the avirulent isolate, 99-56, was performed in order to map the AvrLepR1 gene. The disease reactions of the 94 of the resulting F1 progenies were tested on the canola line ddm-12-6s-1, which carries LepR1. There were 44 avirulent progenies and 50 virulent progenies suggesting a 1:1 segregation ratio and that the avirulence of 99-56 on ddm-12-6s-1 is controlled by a single gene. Tetrad analysis also indicated a 1:1 segregation ratio. The AvrLepR1 gene was positioned on a genetic map of L. maculans relative to 259 sequence-related amplified polymorphism (SRAP) markers, two cloned avirulence genes (AvrLm1 and AvrLm4-7) and the mating type locus (MAT1). The genetic map consisted of 36 linkage groups, ranging in size from 13.1 to 163.7 cM, and spanned a total of 2,076.4 cM. The AvrLepR1 locus was mapped to linkage group 4, in the 13.1 cM interval flanked by the SRAP markers SBG49-110 and FT161-223. The AvrLm4-7 locus was also positioned on linkage group 4, close to but distinct from the AvrLepR1 locus, in the 5.4 cM interval flanked by FT161-223 and P1314-300. This work will make possible the further characterization and map-based cloning of AvrLepR1. A combination of genetic mapping and pathogenicity tests demonstrated that AvrLepR1 is different from each of the L. maculans avirulence genes that have been characterized previously.

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Acknowledgments

The authors thank Dr. Thierry Rouxel and Dr. Marie- Hélène Balesdent for providing seed of lines 22-1-1 and 23-1-1. We also would like to thank Dr. Julian Thomas, Erica Reidel and Sasanda Nilmalgoda, at the Cereal Research Center of Agriculture and Agri-Food Canada, and Paula Parks, at the University of Manitoba, for their technical assistance in this research. This work was supported by an NSERC Discovery Grant from the Government of Canada, awarded to W.G.D. Fernando, and funding from the Agri-Food Research & Development Initiative (ARDI), Government of Manitoba.

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  1. Kaveh Ghanbarnia

    Present address: Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, S7N 0X2, Canada

  2. H. Randy Kutcher

    Present address: Crop Development Centre, College of Agriculture and Bioresources, University of Saskatchewan, 3C02 Agriculture Building, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

Authors and Affiliations

  1. Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Kaveh Ghanbarnia, Genyi Li, Peter B. E. McVetty & W. G. Dilantha Fernando

  2. Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, S7N 0X2, Canada

    Derek J. Lydiate, S. Roger Rimmer & Nicholas J. Larkan

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  1. Kaveh Ghanbarnia
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Correspondence to W. G. Dilantha Fernando.

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Communicated by H. Becker.

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Ghanbarnia, K., Lydiate, D.J., Rimmer, S.R. et al. Genetic mapping of the Leptosphaeria maculans avirulence gene corresponding to the LepR1 resistance gene of Brassica napus . Theor Appl Genet 124, 505–513 (2012). https://doi.org/10.1007/s00122-011-1724-3

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  • DOI: https://doi.org/10.1007/s00122-011-1724-3

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