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Improved degradation of 4-chlorobiphenyl, 2,3-dihydroxybiphenyl, and catecholic compounds by recombinant bacterial strains

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Abstract

ThepcbC gene encoding (4-chloro-)2,3-dihydroxybiphenyl dioxygenase was cloned from the genomic DNA ofPseudomonas sp. P20 using pKT230 to construct pKK1. A recombinant strain,E. coli KK1, was selected by transforming the pKK1 intoE. coli XL1-Blue. Another recombinant strain,Pseudomonas sp. DJP-120, was obtained by transferring the pKK1 ofE. coli KK1 intoPseudomonas sp. DJ-12 by conjugation. Both recombinant strains showed a 23.7 to 26.5 fold increase in the degradation activity to 2,3-dihydroxybiphenyl compared with that of the natural isolate,Pseudomonas sp. DJ-12. The DJP-120 strain showed 24.5, 3.5, and 4.8 fold higher degradation activities to 4-chlorobiphenyl, catechol, and 3-methylcatechol than DJ-12 strain, respectively. The pKK1 plasmid of both strains and their ability to degrade 2,3-dihydroxybiphenyl were stable even after about 1,200 generations.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Chungbuk National University, 361-763, Cheongju, Korea

    Ji-Young Kim & Chi-Kyung Kim

  2. Research Institute for Genetic Engineering, Chungbuk National University, 361-763, Cheongju, Korea

    Ji-Young Kim & Chi-Kyung Kim

  3. College of Pharmacy, Chungbuk National University, 361-763, Cheongju, Korea

    Youngsoo Kim

  4. Department of Microbiology, Changwon National University, 641-773, Changwon, Korea

    Kyoung Lee

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  1. Ji-Young Kim
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  2. Youngsoo Kim
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Correspondence to Chi-Kyung Kim.

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Kim, JY., Kim, Y., Lee, K. et al. Improved degradation of 4-chlorobiphenyl, 2,3-dihydroxybiphenyl, and catecholic compounds by recombinant bacterial strains. Biotechnol. Bioprocess Eng. 6, 56–60 (2001). https://doi.org/10.1007/BF02942251

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  • DOI: https://doi.org/10.1007/BF02942251

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