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Dendritic cells in glomerulonephritis

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Virchows Archiv B

Summary

Renal biopsies (n = 45) from patients with various forms of glomerulonephritis (GN), comprising mesangial IgA-GN (n = 25), focal glomerular sclerosis (n = 13) and acute GN (n = 7), were examined by double staining immunocytochemistry (APAAP, streptavidinperoxidase) using unconjugated monoclonal antibodies (Ab) against (i) the CD1b antigen expressed on dendritic cells (DCs), (ii) the invariant chain (Ii), and (iii) biotin-conjugated Ab against HLA-DR. In normal control kidneys (n = 7) without interstitial inflammation, CD1b-positive DCs were not detected. Glomerular endothelial cells and a few cells in mesangial areas showed double staining with the Ab against HLA-DR in Ii. In GN without active interstitial inflammation (n = 9), CD1b-positive DCs were not found. In biopsies with interstitial inflammation (n = 36) CDlb-positive DCs were found interspersed among other inflammatory cells. In seven of the biopsies showing IgA-GN DCs were seen in the vicinity of those glomeruli that exhibited either crescents or glomerular sclerosis with splitting of Bowman’s capsule. In proximal tubular epithelial cells de novo expression of HLA-DR/Ii-chain was only seen when DCs were present. We conclude that in different forms of GN: (i) CDlb-positive DCs play an important role in the development of interstitial inflammation, and (ii) their presence may be related to the de novo coexpression of HLA-DR/Ii in tubular epithelial cells, possibly mediated through the production of interferon γ and other cytokines.

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References

  • Aiello FB, Longo DL, Overtoil R, Takacs L, Durum SK (1990) A role of cytokines in antigen presentation: IL-1 and IL-4 induce accessory functions of antigen presenting cells. J Immunol 144:2572–2581

    PubMed  CAS  Google Scholar 

  • Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L (1989) Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron 53:41–49

    PubMed  CAS  Google Scholar 

  • Austyn JM (1987) Lymphoid dendritic cells. Immunology 62:161–180

    PubMed  CAS  Google Scholar 

  • Austyn JM, Larsen CP (1990) Migration patterns of dendritic leukocytes. Transplantation 49:1–7

    Article  PubMed  CAS  Google Scholar 

  • Baldwin III WM, Claas FHJ, van Es LA, van Rood JJ (1981) Distribution of endothelial-monocyte and HLA antigens on renal vascular endothelium. Transpl Proc 13:103–107

    Google Scholar 

  • Ballardini G, Bianchi FB, Mirakan R, Fallani M, Pisi E, Bottazzo GF (1987) HLA-A, B, C and HLA-D/DQ expression on unfixed liver biopsy sections from patients with chronic liver disease. Clin Exp Immunol 70:35–46

    PubMed  CAS  Google Scholar 

  • Bannister KM, Ulich TR, Wilson CB (1988) Immunological mechanisms in experimental models of tubulo-interstitial nephritis. In: Davison AM (ed) Nephrology. Baillére Tindall, London Philadelphia Toronto, 618–634

    Google Scholar 

  • Bardadin KA, Desmet VJ (1985) Interdigitating and dendritic reticulum cells in chronic active hepatitis. Histopathology 8:657–667

    Article  Google Scholar 

  • Barrett M, Milton AD, Barrett J, Taube D, Bewick M, Parsons VP, Fabre JW (1987) Needle biopsy evaluation of class II major histocompatibility complex antigen expression for the differential diagnosis of cyclosporine nephrotoxicity from kidney graft rejection. Transplantation 44:223–227

    Article  PubMed  CAS  Google Scholar 

  • Bohle A, Mackensen-Haen S, von Gise H, Grund K-E, Wehrmann M, Batz CH, Bogenschütz Q, Schmitt H, Nagy J, Müller C, Müller G (1990) The consequences of tubulo-interstitial changes for renal function in glomerulopathies. Path Res Pract 186:135–144

    PubMed  CAS  Google Scholar 

  • Bottazzo GF, Pujol-Borrell R, Hanafusa T (1983) Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet 2:1115–1118

    Article  PubMed  CAS  Google Scholar 

  • Boucher A, Droz D, Adafer E, Nöel L-H (1986) Characterization of mononuclear cell subsets in renal cellular interstitial infiltrates. Kidney Int 29:1043–1049

    Article  PubMed  CAS  Google Scholar 

  • Boyd AW (1987) Human leukocyte antigens: an update on structure, function and nomenclature. Pathology 19:329–337

    Article  PubMed  CAS  Google Scholar 

  • Claesson-Welsh I, Peterson PA (1985) Implications of the invariant γ-chain on the intracellular transport of the class II histocompatibility antigens. J Immunol 135:3551–3557

    PubMed  CAS  Google Scholar 

  • D’Amico G (1988) Role of interstitial infiltration of leukocytes in glomerular disease. Nephrol Dial Transplant 3:596–600

    PubMed  CAS  Google Scholar 

  • Degener T, Momburg F, Möler P (1988) Differential expression of HLA-DR, HLA-DP, HLA-DQ and associated invariant chain (Ii) in normal colorectal mucosa, adenoma and carcinoma. Virchows Arch [A] 412:315–322

    CAS  Google Scholar 

  • Demetris AJ, Sever C, Kakizoe S, Oguma S, Starzl TE, Jaffe R (1989) S100 protein positive dendritic cells in primary biliary cirrhosis and other chronic inflammatory liver diseases. Am J Pathol 134:741–747

    PubMed  CAS  Google Scholar 

  • Eberlein-Gonska M, Sill H, Waldherr R (1989) Expression von Klasse I und II Histokompatibilitätsantigenen bei entzündlichen Nierenerkrankungen. Verh Dtsch Ges Pathol73:117–123

    PubMed  CAS  Google Scholar 

  • Epstein AL, Marder RJ, Winter JN, Fox RI (1984) Two new monoclonal antibodies (LN-1, LN-2) reactive in B-5 formalin fixed, paraffin embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol 133:1028–1036

    PubMed  CAS  Google Scholar 

  • Erb P, Ramila G, Studer S, Loeffler H, Cecka JM, Conscience JF, Feldman M (1984) Heterogeneity of accessory cells. Immunobiol 168:1412–1453

    Google Scholar 

  • Fossum S, Ford WL (1985) The organisation of cell populations within lymph nodes: their origin, life history and functional relationship. Histopathology 9:469–499

    Article  PubMed  CAS  Google Scholar 

  • Fossum S (1989) The life history of dendritic leukocytes (DL). In: Iversen OH (ed) Cell kinetics of the inflammatory reaction. Springer, Berlin Heidelberg New York, 101–124

    Google Scholar 

  • Franklin WA, Mason DY, Pulford K, Falini B, Bliss E, Gatter KC, Stein H, Clarke LC, McGree JO’D (1986) Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest 54:322–335

    PubMed  CAS  Google Scholar 

  • Fuggle SV, Errasti P, Daar AS, Fabre JW, Ting A, Morris PJ (1983) Localization of major histocompatibility complex (HLAABC and DR) antigens in 46 kidneys. Transplantation 35:385–390

    Article  PubMed  CAS  Google Scholar 

  • Grey HM, Chesnut RW, Shimaukevtz R, Marrack P, Kappler J (1984) Mechanismus of antigen processing and presentation. Immunobiol 168:202–212

    CAS  Google Scholar 

  • Guargliardi LE, Koppelman B, Blum JS, Marks MS, Cresswell P, Brodsky FM (1990) Co-localization of molecules involved in antigen processing and presentation in an early endocytic compartement. Nature 343:133–139

    Article  Google Scholar 

  • Hall BM, Bishop GA, Duggin GG, Horvath JS, Philips J, Tiller DJ (1984) Increased expression of HLA-DR antigens on renal tubular cells in renal transplants: relevance to the rejection response. Lancet 2:247–251

    Article  PubMed  CAS  Google Scholar 

  • Halloran PF, Autenried P, Wadgymar A (1986) Regulation of HLA antigen expression in human kidney. Clin Immunol Allergy 6:411–435

    Google Scholar 

  • Hämmerling GJ, Moreno J (1990) The function of the invariant chain in antigen presentation by MHC class II molecules. Immunol Today 11:337–340

    Article  PubMed  Google Scholar 

  • Hancock WW, Kraft N, Atkins RC (1982) The immunohistochemical demonstration of major histocompatibility antigens in the human kidney using monoclonal antibodies. Pathology 14:409–414

    Article  PubMed  CAS  Google Scholar 

  • Hart DNJ, Fuggle SV, Williams KA, Fabre JW, Ting A, Morris PJ (1981) Localization of HLA-ABC and DR antigens in humen kidney. Transplantation 31:428–433

    PubMed  CAS  Google Scholar 

  • Häyry P, von Willebrand E, Andersson LC (1980) Expression of HLAABC and DR locus antigens on human kidney, endothelial, tubular and glomerular cells. Scand J Immunol 11:303–310

    Article  PubMed  Google Scholar 

  • Hinglais N, Kazatchkine MD, Charron DJ, Appay M-D, Mandet C, Paing M, Bariety J (1984) Immunohistochemical study of la antigen in the normal and diseased human kidney. Kidney Int 25:544–550

    Article  PubMed  CAS  Google Scholar 

  • Hirschberg H, Scott H, Thorsby E (1981) Human endothelial cells can present antigen to sensitized T-lymphocytes in vitro. Transplant Proc 13:100–102

    PubMed  CAS  Google Scholar 

  • Hooke DH, Gee DC, Atkins RC (1987) Leukocyte analysis using monoclonal antibodies in human glomerulonephritis. Kidney Int 31:964–972

    Article  PubMed  CAS  Google Scholar 

  • Hopkins J, Dutia BM, Bujdoso R, McConnell I (1989) In vivo modulation of CD1 and MHC class II expression by sheep afferent lymph dendritic cells. J Exp Med 170:1303–1318

    Article  PubMed  CAS  Google Scholar 

  • Inaba K, Steinman RM (1984) Resting and sensitized T lymphocytes exhibit distinct stimulating (antigen presenting cell) requirements for growth and lymphokine release. J Exp Med 160:1717–1735

    Article  PubMed  CAS  Google Scholar 

  • Inaba K, Steinman RM (1985) Protein-specific helper T-lymphocyte formation intiated by dendritic cells. Science 229:475–479

    Article  PubMed  CAS  Google Scholar 

  • Inaba K, Young JW, Steinman RM (1987) Direct activation of CD8+ cytotoxic T lymphocytes by dendritic cells. J Exp Med 166:182–194

    Article  PubMed  CAS  Google Scholar 

  • Inaba K, Metlay JP, Crowley MT, Steinman RM (1990) Dendritic cells pulsed with antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ. J Exp Med 172:631–640

    Article  PubMed  CAS  Google Scholar 

  • Kaplan G, Nusrat A, Witmer MD, Nath I, Cohn ZA (1987) Distribution and turnover of Langerhans cells during delayed immune responses in human skin. J Exp Med 165:763–776

    Article  PubMed  CAS  Google Scholar 

  • Kapsenberg ML, Tennissen MBM, Stiekema FEM, Keizer HG (1986) Antigen presenting cell function of dendritic cells and macrophages in proliferative T cell responses to soluble and particulate antigens. Eur J Immunol 16:345–350

    Article  PubMed  CAS  Google Scholar 

  • Knight SC (1984) Veiled cells “dendritic cells” of peripheral lymph. Immunobiol 168:349–361

    CAS  Google Scholar 

  • Knight SC, Krejci J, Malkovsky M, Colizzi V, Gautam A, Asherson GL (1985) The role of dendritic cells in the initiation of immune responses to contact sensitizers. I. In vivo exposure to antigen. Cell Immunol 94:427–137

    Article  PubMed  CAS  Google Scholar 

  • Koene RAP, de Waal RMW, Bogman MJJT (1986) Variable expression of major histocompatibility antigens: role in transplantation immunology. Kidney Int 30:1–8

    Article  PubMed  CAS  Google Scholar 

  • Koretz K, Momburg F, Otto HF, Möller P (1987) Sequential induction of MHC Antigens on autochthonous cells of ileum affected by Crohn’s disease. Am J Pathol 129:493–502

    PubMed  CAS  Google Scholar 

  • Lampson LA, Levy R (1980) Two populations of la-like molecules on a human B cell line. J Immunol 125:293–299

    PubMed  CAS  Google Scholar 

  • Long EO (1989) Intracellular traffic and antigen processing. Immunol Today 10:232–234

    Article  PubMed  CAS  Google Scholar 

  • Mandsley DJ, Pound JD (1991) Modulation of MHC antigen expression by viruses and oncogenes. Immunol Today 12:429–431

    Article  Google Scholar 

  • Mattila PM, Nietosvaara YA, Ustinov JK, Renkonen RL, Häyry PJ (1989) Antigen expression in different parenchymal cells of rat kidney and heart. Kidney Int 36:228–233

    Article  PubMed  CAS  Google Scholar 

  • Miller J, Germain RN (1986) Efficient cell surface expression of class II MHC molecules in the absence of invariant chain. J Exp Med 164:1478–1489

    Article  PubMed  CAS  Google Scholar 

  • Mishra BB, Poulter LW, Janossy G, James DG (1983) The distribution of macrophage and lymphocyte subsets of sarcoid and Kveim test granulomas suggests an immunoregulatory disorder causing negative PPD reaction. Clin Exp Immunol 54:705–715

    PubMed  CAS  Google Scholar 

  • Momburg F, Möller P (1988) Non-co-ordinate expression of HLADR antigens and invariant chain. Immunol 63:551–553

    CAS  Google Scholar 

  • Momburg F, Koretz K, von Herbay A, Möller P (1988) Nonimmune human cells can express MHC class II antigens in the absence of invariant chain an immunohistological study on normal and chronically inflamed small intestine. Clin Exp Immunol 72:367–372

    PubMed  CAS  Google Scholar 

  • Müller CA, Markovic-Lipkovski J, Risler T, Müller GA (1989) Expression of HLA-DQ,-DR, and -DP antigens in normal kidney and glomerulonephritis. Kidney Int 35:116–124

    Article  PubMed  Google Scholar 

  • Nagata K, Platt JL, Michael AF (1984) Interstitial and glomerular immune cell populations in idiopathic nephrotic syndrome. Kidney Int 25:88–93

    Article  PubMed  CAS  Google Scholar 

  • Natali PG, De Martino C, Marcellini M, Quaranta V, Ferrone S (1981) Expression of la-like antigens on the vasculature of human kidney. Clin Immunol Immunopathol 20:11–20

    Article  PubMed  CAS  Google Scholar 

  • Nolasco FEB, Cameron JS, Hartley B, Coelho A, Hildreth G, Ruben R (1987) Intraglomerular T cells and monocytes in nephritis: study with monoclonal antibodies. Kidney Int 31:1160–1166

    Article  PubMed  CAS  Google Scholar 

  • Olive D, Dubreuil P, Mawas C (1984) Two distinct TL like molecular subsets defined by monoclonal antibodies on the surface of human thymocytes with different expressions on leukemia lines. Immunogenetics 20:253–254

    Article  PubMed  CAS  Google Scholar 

  • Parwaresch MR, Radzun HJ, Feller AC, Peters KP, Hansmann ML (1983) Peroxidase positive mononuclear leukocytes as possible precursors of human dendritic reticulum cells. J Immunol 131:2719–2725

    PubMed  CAS  Google Scholar 

  • Platt JL, Grant BW, Eddy AA, Michael AF (1983) Immune cell population of cutaneous delayed type hypersensitivity. J Exp Med 158:1227–1242

    Article  PubMed  CAS  Google Scholar 

  • Poulter LW (1983) Antigen presenting cells in situ: their identification and involvement in immunopathology. Clin Exp Immunol 53:513–520

    PubMed  CAS  Google Scholar 

  • Poulter LW, Duke O, Hobbs S, Janossy G, Panayi G, (1983) The involvement of interdigitating (antigen presenting) cells in the pathogenesis of rheumatoid arthritis. Clin Exp Immunol 51:247–254

    PubMed  CAS  Google Scholar 

  • Renkonen RL, Häyry PJ (1989) Antigen expression in different parenchymal cell types of rat kidney and heart. Kidney Int 36:228–233

    Article  PubMed  Google Scholar 

  • Scott H, Brandtzaeg P, Hirschberg H, Solheim BG, Thorsby E (1981) Vascular and renal distribution of HLA-DR-like antigens. Tissue Antigens 18:195–202

    Article  PubMed  CAS  Google Scholar 

  • Sekaly RP, Tonnelle C, Strubin M, Mach B, Long EO (1986) Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J Exp Med 164:1490–1504

    Article  PubMed  CAS  Google Scholar 

  • Selby WS, Polter LW, Hobbs S, Janossy G, Jewell DP (1983) Heterogeneity of HLA-DR+ve histiocytes in human interstitial lamina propria. A combined histochemical and immunohistologiacal analysis. J Clin Path [Suppl] 36:379–384

    Article  PubMed  CAS  Google Scholar 

  • Silberberg-Sinakin I, Thorbecke GJ (1980) Contact hypersensitivity and Langerhans cells. J Invest Dermatol 75:61–67

    Article  PubMed  CAS  Google Scholar 

  • Stachura I, Si L, Madan E, Whiteside T (1984) Mononuclear cell subsets in human renal disease. Enumeration in tissue sections with monoclonal antibodies. Clin Immunol Immunopathol 30:362–373

    Article  PubMed  CAS  Google Scholar 

  • Steinman RM (1981) Dendritic cells. Transplantation 31:151–155

    Article  PubMed  CAS  Google Scholar 

  • Streilim JW, Bererstresser PR (1984) Langerhans cells: antigen presenting cells of epidermis. Immunobiol 168:285–300

    Google Scholar 

  • Tjernlund U, Scheynius A (1984) Amplified T-cell response to PPD by epidermal cell suspensions containing HLA-DR-expressing keratinocytes. Acta Derm Venerel (Stockh) [Suppl] 135:57–57

    Google Scholar 

  • Van Voorhis WCV, Hair LS, Steinman RM, Kaplan G (1982) Human dendritic cells: enrichment and characterization from peripheral blood. J Exp Med 155:1172–1187

    Article  PubMed  Google Scholar 

  • Wadgymar A, Ritchie S, Cattran DC, Fenton S, Halloran PF (1987) Patterns of HLA antigen expression in human kidney disease. Transplant Proc 19:3410–3414

    PubMed  CAS  Google Scholar 

  • Waldherr R, Raumbausek M, Noronha I, Ritz E (1989) MHC class I and II antigen expression in renal disease an immunohistochemical study on kidney biopsies [Abstr]. Kidney Int 35:376

    Google Scholar 

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  1. Departments of Pathology, University of Heidelberg, Federal Republic of Germany

    Snjezana Cuzic & Rüdiger Waldherr

  2. Internal Medicine, University of Heidelberg, Federal Republic of Germany

    Eberhard Ritz

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  1. Snjezana Cuzic
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  2. Eberhard Ritz
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Supported by the Deutsche Forschungsgemeinschaft (Wa 698/2-1)

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Cuzic, S., Ritz, E. & Waldherr, R. Dendritic cells in glomerulonephritis. Virchows Archiv B Cell Pathol 62, 357–363 (1992). https://doi.org/10.1007/BF02899704

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