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Immunochemical analysis of common antigenic determinants in insulin and apoprotein B Molecules

  • Biophysics and Biochemistry
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A study is performed of the mechanism underlying stress diabetes, which develops in human beings and animals under stress. Dot-immunoanalysis shows the presence of common antigenic determinants in insulin, apoprotein B, and apoprotein B-containing low density and very low density lipoproteins isolated from human and rat serum. Electrophoresis, immunoelectroblotting, and immunoenzyme analysis reveal 5–6 peptides belonging to apoB, which specifically react with anti-insulin and anti-very low density lipoprotein antibodies. Insulinlike immunoreactivity is also identified in human serum supernatant obtained after precipitation of the total low density and very low density lipoprotein fraction and after removal all lipoproteins from it.

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Authors and Affiliations

  1. Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk

    L. E. Panin, L. S. Ostanina & N. V. Atuchina

Authors
  1. L. E. Panin
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  2. L. S. Ostanina
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  3. N. V. Atuchina
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Additional information

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, No 9, pp. 258–261, September, 1994

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Panin, L.E., Ostanina, L.S. & Atuchina, N.V. Immunochemical analysis of common antigenic determinants in insulin and apoprotein B Molecules. Bull Exp Biol Med 118, 956–959 (1994). https://doi.org/10.1007/BF02445783

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  • DOI: https://doi.org/10.1007/BF02445783

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