Skip to main content
SpringerLink
Log in

Ionophoric material derived from eel membrane preparation

I. Chemical characteristics

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

Prolonged digestion with trypsin released ionophoric material from a preparation of microsomes obtained fromElectrophorus electricus. The material induced a large increase in the conductance of a black lipid membrane prepared from oxidized cholesterol. The induced conductance of cations was greater than that of anions. There was no discrimination between monovalent inorganic cations, neither with respect to conductance nor with respect to rate of incorporation. Gel filtration indicated a molecular weight of about 2000 for the active material. The material was 50- to 200-fold more effective when applied to the membrane on both sides than when applied on only one side. The material was unstable to storage, particularly at pH levels far from enutrality, but was partially reactivated in some cases by lyophilization or by a high concentration of dithiothereitol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albers, R. W., Fahn, S., Koval, G. J. 1963. The role of sodium ions in the activation ofElectrophorus electric organ adenosine triphosphatase.Proc. Nat. Acad. Sci. 50:474

    PubMed  Google Scholar 

  • Blumenthal, R., Shamoo, A. E. 1974. Ionophoric material derived from eel membrane preparations. II. Electrical characteristics.J. Membrane Biol. 19:141

    Google Scholar 

  • Cass, A., Finkelstein, A., Krespi, V. 1970. The ion permeability in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B.J. Gen. Physiol. 56:100

    Article  PubMed  Google Scholar 

  • Cleland, W. W. 1964. Dithiothreitol, a new protective reagent for SH groups.Biochemistry 3:480

    Google Scholar 

  • Habeeb, A. F. S. A. 1972. Reaction of protein sulfhydryl groups with Ellman's reagent.In: Methods in Enzymology. C. H. W. Hirs and S. N. Timasheff, editors. Vol. 25, p. 457. Academic Press, New York

    Google Scholar 

  • Harigaya, S., Schwartz, A. 1969. Rate of calcium binding and uptake in normal animal and failing human cardiac muscle.Circulation Res. 25:781

    PubMed  Google Scholar 

  • Jain, M. K., White, F. P., Strickholm, A., Williams, E., Cordes, E. H. 1972. Studies concerning the possible reconstitution of an active cation pump across an artificial membrane.J. Membrane Biol. 8:363

    Google Scholar 

  • Katz, A. M., Toda, M., Repke, D. O., Iorio, J. A. M., Kirchberger, M. A. 1974. Adenylate cyclase: Its probable localization in the sarcoplasmic reticulum as well as sarcolemma of canine heart.J. Molec. Cell. Card. 6:73

    Google Scholar 

  • Lattore, R., Ehrenstein, G., Lecar, H. 1972. Ion transport through excitability-inducing material (EIM) channels in lipid bilayer membranes.J. Gen. Physiol. 60:72

    PubMed  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265

    PubMed  Google Scholar 

  • Neumann, N. P. 1972. Oxidation with hydrogen peroxide.In: Methods in Enzymology. C. H. W. Hirs and S. N. Timasheff, editors. Vol. 25, p. 393. Academic Press, New York

    Google Scholar 

  • Riordan, J. F., Valee, B. L. 1972. Reactions with N-ethyl-malemide andp-mercuribenzoate.In: Methods in Enzymology. C. H. W. Hirs and S. N. Timasheff, editors. Vol. 25, p. 449. Academic Press, New York

    Google Scholar 

  • Sachs, G., Spenney, J. G., Saccomani, G., Goodall, M. C. 1974. Characterization of gastric mucosal membranes. VI. The presence of channel forming substances.Biochim. Biophys. Acta 332:233

    Google Scholar 

  • Shamoo, A. E., Albers, R. W. 1973. Na+-selective ionophoric material derived from electric organ and kidney membranes.Proc. Nat. Acad. Sci. 70:1191

    PubMed  Google Scholar 

  • Shamoo, A. E., Brodsky, W. A. 1972. Functions of the E-ATP and E-P complexes in the membrane ATPase reaction.Biochim. Biophys. Acta 255:220

    PubMed  Google Scholar 

  • Siegel, G. J., Albers, R. W. 1970. Nucleoside triphosphate phosphohydrolases.In: Handbook of Neurochemistry. A. Lajtha, editor. Vol. 4, p. 13. Plenum Press, New York

    Google Scholar 

  • Skou, J. C. 1965. Enzymatic basis for active transport of Na+ and K+ across cell membrane.Physiol. Rev. 45:596

    PubMed  Google Scholar 

  • Tien, H. T., Carbone, S., Davidowicz, E. A. 1966. Formation of “black” lipid membranes by oxidation products of cholesterol.Nature 212:719

    Google Scholar 

  • Urry, D. W., Goodall, M. C., Glickson, J. D., Mayers, D. F. 1971. The Gramicidin A transmembrane channel: Characteristics of head-to-head dimerizedπ (LiD) helices.Proc. Nat. Acad. Sci. 68:1907

    PubMed  Google Scholar 

  • Westhead, E. W. 1972. Dye-sensitized photooxidation.In: Methods in Enzymology. C. H. W. Hirs and S. N. Timasheff, editors. Vol. 25, p. 401. Academic Press, New York

    Google Scholar 

  • White, F. H., Jr. 1972. Reduction and reoxidation at disulfide bonds.In: Methods in Enzymology. C. H. W. Hirs and S. N. Timasheff, editors. Vol. 25, p. 387. Academic Press, New York

    Google Scholar 

  • Zamura, Z., Nakajimia, T., Nakayama, T., Pisano, J. J., Udenfriend, S. 1973. Identification of peptides with 5-dimethylamino-naphthalenesulfonyl chloride.Analyt. Biochem. 52:595

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, University of Rochester, School of Medicine and Dentistry, 14642, Rochester, New York

    Adil E. Shamoo, Marjory M. Myers, Robert Blumenthal & R. Wayne Albers

  2. Laboratory of Theoretical Biology, National Cancer Institute, National Institutes of Health, 20014, Bethesda, Maryland

    Adil E. Shamoo, Marjory M. Myers, Robert Blumenthal & R. Wayne Albers

  3. Laboratory of Neurochemistry, National Institutes of Neurological Diseases and Stroke, National Institutes of Health, 20014, Bethesda, Maryland

    Adil E. Shamoo, Marjory M. Myers, Robert Blumenthal & R. Wayne Albers

Authors
  1. Adil E. Shamoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marjory M. Myers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert Blumenthal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Wayne Albers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shamoo, A.E., Myers, M.M., Blumenthal, R. et al. Ionophoric material derived from eel membrane preparation. J. Membrain Biol. 19, 129–140 (1974). https://doi.org/10.1007/BF01869974

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01869974

Keywords

Search

Navigation