Summary
Mechanically dissociated brain cells of 14 and 18-day-old mouse embryos and of mouse neonates were cultured for 3 weeks. Neurons, oligodendrocytes and astrocytes were identified at the 7th, 14th and 21st day in vitro by staining the cultures using the indirect immunoperoxidase technique with antisera directed against neuron specific enolase, galactocerebroside, myelin basic protein and glial fibrillary acidic protein. The number of neurons and oligodendrocytes was higher in embryonic cultures than in neonate cultures. The expression of some antigens was also different in the two types of culture. Our results indicate that the development of brain cells in mechanically dissociated brain cell cultures depends on the age of the animal at the time of plating.
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Supported by grant 3.717.80 from the Swiss National Research Foundation, a grant from the Swiss Multiple Sclerosis Society and a grant from the Swiss Foundation of Encouragement of Research in Mental Retardation
L. Bologa was supported by INSERM (No. 81/007) during her stay in Paris
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Bologa, L., Joubert, R., Bisconte, JC. et al. Development of immunologically identified brain cells in culture: Quantitative aspects. Exp Brain Res 53, 163–167 (1983). https://doi.org/10.1007/BF00239408
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DOI: https://doi.org/10.1007/BF00239408