Abstract
Bisulfite sequencing is the “gold-standard” technique for DNA methylation analysis. By combining bisulfite sequencing with high-throughput, next-generation sequencing technology, we can document methylation from many thousands of individual reads (equivalent to alleles or “cells”), for multiple target regions and from many samples simultaneously. Here, we describe a next-generation bisulfite-sequencing assay for targeted DNA methylation analysis which offers scope for the simultaneous interrogation of multiple genomic loci across numerous samples.
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Acknowledgments
We would like to acknowledge Professor Ian Morison, as the “driving force” behind the development of this high-throughput, next-generation bisulfite sequencing protocol and analysis workflow. While this protocol is the work of many, contributions from Jackie Ludgate, Luke Bridgman, Dr. Suzan Almomani, and Dr. Issam Mayyas were instrumental in developing the bisulfite sequencing protocol and the analysis workflow.
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Smith, J., Day, R.C., Weeks, R.J. (2022). Next-Generation Bisulfite Sequencing for Targeted DNA Methylation Analysis. In: Horsfield, J., Marsman, J. (eds) Chromatin. Methods in Molecular Biology, vol 2458. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2140-0_3
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DOI: https://doi.org/10.1007/978-1-0716-2140-0_3
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