Abstract
Aedes aegypti L. is a primary vector of dengue and is also responsible for transmission of other arboviral diseases including chikungunya, yellow fever, and Zika virus worldwide. India is attributed with as much as 34% of the global burden of dengue infection as per the recent reports. It is generally acknowledged controlling the vector population in the environment is the key to effectively curb the transmission of vector-borne diseases. Existing conventional methods for controlling the vector populations are not alone sufficient for reducing populations below disease transmission thresholds and would need integration of innovative strategies. With the advancement in modern biotechnology, scientists have been trying to explore the use of genetically engineered insects for their potential use in area-wide method for vector control. One among such strains being widely tested is OX513A Ae. aegypti strain termed as Friendly™ mosquitoes, a genetically engineered strain with a repressible dominant homozygous lethal gene construct and DsRed2 fluorescent marker gene. Male adults of OX513A strain are intended to suppress the wild population of Ae. aegypti in the released environment by population suppression strategy. The lethal gene renders mating events between OX513A males and wild-type females unsuccessful by passing a copy of gene to all the progeny of mated female adults leading to the death of the progeny during development stages in the absence of repressor antidote tetracycline. This OX513A strain was reviewed and recommended for carefully planned pilot deployment and randomized control trials with epidemiological outcomes to build evidence for routine programmatic use against Ae. aegypti-borne diseases by World Health Organization’s Vector Control Advisory Group in the year 2016. The strain is being tested globally in different countries including India for its efficacy and has been deregulated in Brazil for its use in vector control program. This chapter reviews our research investigations conducted on transgenic OX513A Ae. aegypti strain and its potential for use in dengue vector control in India.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Alphey L, McKemey A, Nimmo D et al (2013) Genetic control of Aedes mosquitoes. Pathog Glob Health 107(4):170–179. https://doi.org/10.1179/2047773213Y.0000000095
Arthropod Containment Levels (2003) Vector Borne Zoonotic Dis 3:75–90
Avise JC, Arnold J, Ball RM et al (1987) Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu Rev Ecol Syst 18:489–522. https://doi.org/10.1146/annurev.es.18.110187.002421
Azil AH, Long SA, Ritchie SA et al (2010) The development of predictive tools for preemptive dengue vector control: a study of Aedes aegypti abundance and meteorological variables in North Queensland, Australia. Tropical Med Int Health 15:1190–1197. https://doi.org/10.1111/j.1365-3156.2010.02592.x
Barraud PJ (1934) The Fauna of British India, including Ceylon and Burma. In: Diptera. Family Culieldae. Tribes Megarhinini and Culicini, vol 5. Taylor & Francis, London, pp 1–463
Beech CJ, Nagaraju J, Vasan SS et al (2009) Risk analysis of a hypothetical open field release of a self-limiting transgenic Aedes aegypti mosquito strain to combat dengue. Asia Pac J Mol Biol Biotechnol 17(3):99–111
Bhardwaj S, Gokhale MD, Mourya DT (2017) Zika virus: current concerns in India. Indian J Med Res 146(5):572–575. https://doi.org/10.4103/ijmr.ijmr_1160_17
Bhatt S, Gething PW, Brady OJ et al (2013) The global distribution and burden of dengue. Nature 496:504–507. https://doi.org/10.1038/nature12060
Brady OJ, Gething PW, Bhatt S et al (2012) Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl Trop Dis 6:e1760. https://doi.org/10.1371/journal.pntd.0001760
Carvalho DO, Mckemey AR, Garziera L et al (2015) Suppression of a field population of Aedes aegypti in Brazil by sustained release of transgenic male mosquitoes. PLoS Negl Trop Dis 9(7):e0003864. https://doi.org/10.1371/journal.pntd.0003864
Cecilia D (2014) Current status of dengue and chikungunya in India. WHO South East Asia J Public Health 3(1):22–26. https://doi.org/10.4103/2224-3151.206879
Chakravarti A, Arora R, Luxemburger C (2012) Fifty years of dengue in India. Trans R Soc Trop Med Hyg 106:273–282. https://doi.org/10.1016/j.trstmh.2011.12.007
Chaturvedi UC, Nagar R (2008) Dengue and dengue haemorrhagic fever: Indian perspective. J Biosci 33:429–441. https://doi.org/10.1007/s12038-008-0062-3
Craig GB Jr (1967) Mosquitoes: female monogamy induced by male accessory gland substance. Science 156(781):1499–1501. https://doi.org/10.1126/science.156.3781.1499
Curtis CF, Sinkins SP (1998) Wolbachia as a possible means of driving genes into populations. Parasitology 116(Suppl):S111–S115. https://doi.org/10.1017/s0031182000084997
Dhiman S, Rabha B, Yadav K et al (2014) Insecticide susceptibility and dengue vector status of wild Stegomyia albopicta in a strategically important area of Assam, India. Parasites Vectors 7(295):1–5. https://doi.org/10.1186/1756-3305-7-295
Enserink M (2006) Infectious diseases. Massive outbreak draws fresh attention to little-known virus. Science 311:1085. https://doi.org/10.1126/science.311.5764.1085a
Focks DA, UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (2004) A review of entomological sampling methods and indicators for dengue vectors. World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/68575
Galtier N, Nabholz B, Giemin S et al (2009) Mitochondrial DNA as a marker of molecular diversity: a reappraisal. Mol Ecol 18:4541–4550. https://doi.org/10.1111/j.1365-294X.2009.04380.x
Gandhi R, Yadav KK, Patil PB et al (2020) Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes. J Asia Pac Entomol 23:51–59. https://doi.org/10.1016/j.aspen.2019.10.006
Gong P, Epton M, Fu G et al (2005) A dominant lethal genetic system for autocidal control of the Mediterranean fruitfly. Nat Biotechnol 23:453–456. https://doi.org/10.1038/nbt1071
Gupta B, Reddy BP (2013) Fight against dengue in India: progresses and challenges. Parasitol Res 112:1367–1378. https://doi.org/10.1007/s00436-013-3342-2
Gupta N, Srivastava S, Jain A, Chaturvedi UC (2012) Dengue in India. Indian J Med Res 136:373–390. https://www.ijmr.org.in/text.asp?2012/136/3/373/101976
Halstead SB (2007) Dengue. Lancet 370:1644–1652. https://doi.org/10.1016/s0140-6736(07)61687-0
Harris AF, Nimmo D, McKemey AR et al (2011) Field performance of engineered male mosquitoes. Nat Biotechnol 29(11):1034–1037. https://doi.org/10.1038/nbt.2019
Harris AF, Mckemey AR, Nimmo D et al (2012) Successful suppression of a field mosquito population by sustained release of engineered male mosquitoes. Nat Biotechnol 30(9):828–830. https://doi.org/10.1038/nbt.2350
Harrison BA, Boonyakanist P, Mongkolpanya K (1972) Biological observations on Aedes seatoi Huang in Thailand with notes on rural Aedes aegypti (L.) and other Stegomyia populations. J Med Entomol 9(1):1–6. https://doi.org/10.1093/jmedent/9.1.1
Helinski ME, Harrington J (2011) Male mating history and body size influence female fecundity and longevity of the dengue vector Aedes aegypti. J Med Entomol 48(2):202–211. https://doi.org/10.1603/me10071
Huang Y, Magori K, Lloyd AL et al (2007) Introducing desirable transgenes into insect populations using Y-linked meiotic drive—a theoretical assessment. Evolution 61:717–726. https://doi.org/10.1111/j.1558-5646.2007.00075.x
Irvin N, Hoddle MS, O’Brochta DA et al (2004) Assessing fitness costs for transgenic Aedes aegypti expressing the GFP marker and transposase genes. Proc Natl Acad Sci U S A 101(3):891–896. https://doi.org/10.1073/pnas.0305511101
Kay B, Vu SN (2005) New strategy against Aedes aegypti in Vietnam. Lancet 365(9459):613–617. https://doi.org/10.1016/S0140-6736(05)17913-6
Knipling EF (1992) Brief talk on the occasion of receiving the world food prize on 12 Oct 1992 at Des Moines, Iowa, USA. Unpublished document
Lee HL (1990) Breeding habitats and factors affecting breeding of Aedes larvae in urban towns of Peninsular Malaysia. J Biosci 1(2):107–112
Lee HL (1992) Aedes ovitrap and larval survey in several suburban communities in Selangor, Malaysia. Trop Biomed 9:29–34
Lee H, Vasan S, Nazni W et al (2008) Scientific report on the innovative application of Aedes aegypti RIDL-sterile insect technique to combat dengue and chikungunya in Malaysia. Institute of Medical Research, Malaysia, Kuala Lumpur
Lee HL, Joko H, Nazni WA et al (2009) Comparative life parameters of transgenic and wild strain of Aedes aegypti in the laboratory. Dengue Bull 33:103–114
Li C, Marrelli MT, Yan G et al (2008) Fitness of transgenic Anopheles stephensi mosquitoes expressing the SM1 peptide under the control of a vitellogenin promoter. J Hered 99(3):275–282. https://doi.org/10.1093/jhered/esn004
Lim KW, Sit NW, Norzahira R et al (2010) Dengue vector surveillance in insular settlements of Pulau Ketam, Selangor, Malaysia. Trop Biomed 27(2):185–192
Matthews BJ, Dudchenko O, Kingan SB et al (2018) Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature 563(7732):501–507. https://doi.org/10.1038/s41586-018-0692-z
Mavalankar DV, Puwar TI, Govil D et al (2009) A preliminary estimate of immediate cost of chikungunya and dengue to Gujarat, India. IIMA Working Papers WP2009-01-01, Indian Institute of Management, Ahmedabad, India
Moritz C, Dowling TE, Brown WM (1987) Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Annu Rev Ecol Syst 18:269–292. https://doi.org/10.1146/annurev.es.18.110187.001413
Mutheneni SR, Morse AP, Caminade C et al (2017) Dengue burden in India: recent trends and importance of climatic parameters. Emerg Microbes Infect 6(8):e70. https://doi.org/10.1038/emi.2017.57
Nazni WA, Selvi S, Lee HL et al (2009) Susceptibility status of transgenic Aedes aegypti (L.) against insecticides. Dengue Bull 33:124–129. https://apps.who.int/iris/handle/10665/170922
Norzahira R, Hidayatulfathi O, Wong HM et al (2011) Ovitrap surveillance of the dengue vectors, Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus Skuse in selected areas in Bentong, Pahang, Malaysia. Trop Biomed 28(1):48–54
Patil PB, Reddy BPN, Gorman K et al (2015) Mating competitiveness and life-table comparisons between transgenic and Indian wild-type Aedes aegypti L. Pest Manag Sci 71:957–965. https://doi.org/10.1002/ps.3873
Patil PB, Gorman KJ, Dasgupta SK (2018) Self-limiting OX513A Aedes aegypti demonstrate full susceptibility to currently used insecticidal chemistries as compared to Indian wild-type Aedes aegypti. Psyche J Entomol 2018. https://doi.org/10.1155/2018/7814643
Patil PB, Dasgupta SK, Seshu Reddy KV et al (2020) Surveillance of Aedes mosquito species in villages of Jalna district, Maharashtra. India J Entomol Zool Stud 8(2):669–677
Phuc H, Andreasen MH, Burton RS et al (2007) Late-acting dominant lethal genetic systems and mosquito control. BMC Biol 5:1–11. https://doi.org/10.1186/1741-7007-5-11
Pialoux G, Gaüzère BA, Jauréguiberry S et al (2007) Chikungunya, an epidemic arbovirosis. Lancet Infect Dis 7(5):319–327. https://doi.org/10.1016/s1473-3099(07)70107-x
Ramakrishnan SP, Geljand HM, Bose PN et al (1964) The epidemic of acute haemorrhagic fever, Calcutta, 1963; epidemiological inquiry. Indian J Med Res 52:633–650
Ratnasingham S, Hebert PDN (2007) Bold: the barcode of life data system (http://www.barcodinglife.org). Mol Ecol Notes 7(3):355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
Ravi V (2006) Re-emergence of chikungunya virus in India. Indian J Med Microbiol 24:83–84. https://doi.org/10.4103/0255-0857.25175
Robinson MC (1955) An epidemic of virus disease in Southern Province, Tanganyika Territory, in 1952–53. I. Clinical features. Trans R Soc Trop Med Hyg 49:28–32. https://doi.org/10.1016/0035-9203(55)90080-8
Shepard DS, Halasa YA, Tyagi BK et al (2014) Economic and disease burden of dengue illness in India. Am J Trop Med Hyg 91:1235–1242. https://doi.org/10.4269/ajtmh.14-0002
Sinkins SP, Gould F (2006) Gene drive systems for insect disease vectors. Nat Rev Genet 7:427–435. https://doi.org/10.1038/nrg1870
Sudeep AB, Parashar D (2008) Chikungunya: an overview. J Biosci 33:443–449. https://doi.org/10.1007/s12038-008-0063-2
UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (2015) Biosafety for human health and the environment in the context of the potential use of genetically modified mosquitoes (GMMs): a tool for biosafety training based on courses in Africa, Asia and Latin America, 2008–2011. World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/180388
Watts DM, Burke DS, Harrison BA et al (1987) Effect of temperature on the vector efficiency of Aedes aegypti for dengue 2 virus. Am J Trop Med Hyg 36:143–152. https://doi.org/10.4269/ajtmh.1987.36.143
WHO Special Programme for Research and Training in Tropical Diseases (2014) World Health Organization, guidance framework for testing of genetically modified mosquitoes. World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/127889
World Health Organization (2012) Global strategy for dengue prevention and control, 2012–2020. World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/75303
World Health Organization (2016) Test procedures for insecticide resistance monitoring in malaria vector mosquitoes, 2nd edn. World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/250677
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Patil, P.B., Yadav, K.K., Dasgupta, S.K., Zehr, U.B., Barwale, S.R., Char, B. (2021). Evaluation of Transgenic Aedes aegypti L. Strain in India: A Friendly Mosquito. In: Tyagi, B.K. (eds) Genetically Modified and other Innovative Vector Control Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-16-2964-8_5
Download citation
DOI: https://doi.org/10.1007/978-981-16-2964-8_5
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-16-2963-1
Online ISBN: 978-981-16-2964-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)