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  1. No Access

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    Networking of Smart Drugs: A Chem-Bioinformatic Approach to Cancer Treatment

    Increasing rate of cancer incidence has put a global demand of high and advanced level of diagnosis methods. Although surgery (surgical treatment), radiotherapy, and chemotherapy are well-known traditional met...

    Kavindra Kumar Kesari, Qazi Mohammad Sajid Jamal, Mohd. Haris Siddiqui (2018)

  2. No Access

    Protocol

    Molecular Docking Studies in Multitarget Antitubercular Drug Discovery

    Tuberculosis, caused by Mycobacterium tuberculosis, is an infectious disease with high levels of mortality worldwide, currently with approximately 6.3 million new cases per year that often present resistance to b...

    Jéssika de Oliveira Viana, Marcus T. Scotti, Luciana Scotti (2018)

  3. No Access

    Protocol

    Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

    Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. ...

    Kiran Suhas Nilangekar, Bhupendra V. Shravage (2018)

  4. No Access

    Protocol

    Isolation and Characterization of Cutaneous Epithelial Stem Cells

    The outer layer of mammalian skin is a multilayered epithelium that perpetually renews multiple differentiated lineages. During homeostasis, the maintenance of skin epithelial turnover is ensured by regionaliz...

    Stephanie R. Gillespie, David M. Owens (2018)

  5. No Access

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    Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells

    Human hair follicle-associated pluripotent (hHAP) stem cells were isolated from the upper parts of human hair follicles. hHAP stem cells were suspended in DMEM containing 10% fetal bovine serum (FBS) where the...

    Robert M. Hoffman (2018)

  6. No Access

    Protocol

    Analysis of TrkB Receptor Activity Using FRET Sensors

    Here, we describe the use of 2-photon fluorescence lifetime imaging (2pFLIM) of a Förster resonance energy transfer (FRET) sensor to study the spatial and temporal activity pattern of the BDNF receptor TrkB. C...

    Charles E. Hall, James O. McNamara, Ryohei Yasuda (2018)

  7. No Access

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    Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

    Epidermal stem cells (EpSCs) isolation struggle remains, mainly due to the yet essential requirement of well-defined approaches and markers. The herein proposed methodology integrates an assemblage of strategi...

    Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, Alexandra P. Marques (2018)

  8. No Access

    Protocol

    Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using Single Quantum Dots

    We describe a methodological workflow for detecting and tracking single brain-derived neurotrophic factor (BDNF) receptor complexes in primary cultures of neurons. This methodology includes the preparation of ...

    Anke Vermehren-Schmaedick, Thomas Jacob, Tania Q. Vu (2018)

  9. No Access

    Protocol

    Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

    Tissue engineering-based regenerative applications can involve the use of stem cells for the treatment of non-healing wounds. Multipotent mesenchymal stem cells have become a focus of skin injury treatments al...

    Mahmut Parmaksiz, Ayşe Eser Elçin, Yaşar Murat Elçin (2018)

  10. No Access

    Protocol

    Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

    Autophagy is an important cellular process for maintenance of quality and functionality of cells. This happens through repair and renewal of cellular components like proteins and mitochondria. Reduction in aut...

    Rohan S. Kulkarni, Manmohan S. Bajaj, Vaijayanti P. Kale (2018)

  11. No Access

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    Visualization and Measurement of Multiple Components of the Autophagy Flux

    Autophagy is an intracellular degradation process that mediates the clearance of cytoplasmic components. As well as being an important function for cellular homeostasis, autophagy also promotes the removal of ...

    Tracey Evans, Robert Button, Oleg Anichtchik, Shouqing Luo (2018)

  12. No Access

    Protocol

    Complimentary Methods for Assessing Hypothalamic Actions of Manganese During Pubertal Development

    Utilizing both in vitro and in vivo physiological approaches can be beneficial when trying to obtain critical insights regarding both endogenous and exogenous factors that may control or alter hypothalamic mec...

    William L. Dees, Jill K. Hiney, Vinod K. Srivastava (2018)

  13. No Access

    Protocol

    Historeceptomics: Integrating a Drug’s Multiple Targets (Polypharmacology) with Their Expression Pattern in Human Tissues

    Historeceptomics is a new, integrative informatics approach to describing the mechanism of action of drugs in a holistic, in vivo context. The approach is based on leveraging emerging big data sources in genom...

    Timothy Cardozo (2018)

  14. No Access

    Protocol

    Multitarget Drug Design for Neurodegenerative Diseases

    The quest for new pharmacological treatments of neurodegenerative diseases (NDs) still remains a priority for researchers and caregivers. Being inherently multifactorial, NDs benefited of the paradigm shift fr...

    Marco Catto, Daniela Trisciuzzi, Domenico Alberga, Giuseppe Felice Mangiatordi (2018)

  15. No Access

    Protocol

    Advanced Chemometric Modeling Approaches for the Design of Multitarget Drugs Against Neurodegenerative Diseases

    Neurodegenerative diseases (ND), a major worldwide health problem, present a multifactorial nature. This implies that a multitargeted therapy approach can be considered more effective in such cases when compar...

    Amit Kumar Halder, Ana S. Moura, M. Natália D. S. Cordeiro (2018)

  16. No Access

    Protocol

    Design of Novel Dual-Target Hits Against Malaria and Tuberculosis Using Computational Docking

    Drugs which are purposefully designed to hit more than one target (multi-target drugs) promise a better safety profile and low resistance probability. Multi-target therapy also offers a cost-effective model fo...

    Manoj Kumar, Anuj Sharma (2018)

  17. No Access

    Protocol

    Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

    The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk c...

    Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, Colin Jamora (2018)

  18. No Access

    Protocol

    Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

    An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent sta...

    Edwige Roy, Kiarash Khosrotehrani (2018)

  19. No Access

    Protocol

    Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)

    Age-related macular degeneration (AMD) is the foremost cause of blindness in people over the age of 60 worldwide. Clinically, this disease starts with distortion in central vision eventually leading to legal b...

    Harshini Surendran, Reena J. Rathod, Rajarshi Pal (2018)

  20. No Access

    Protocol

    Cheminformatics Approaches to Study Drug Polypharmacology

    Herein is presented a tutorial overview on selected chemoinformatics methods useful for assembling, curating/preparing a chemical database, and assessing its diversity and chemical space. Methods for evaluatin...

    J. Jesús Naveja, Fernanda I. Saldívar-González, Norberto Sánchez-Cruz (2018)

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