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  1. No Access

    Protocol

    Purification Method for Recombinant hG-CSF by Affinity Chromatography

    The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of...

    Bruna Samham Archangelo, Elisa Maria de Sousa Russo in Recombinant Glycoprotein Production (2018)

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    Protocol

    Modification of Selenoprotein mRNAs by Cap Tri-methylation

    Several selenoprotein mRNAs undergo 5′ cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m3 2,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunop...

    Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang in Selenoproteins (2018)

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    Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies

    Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formula...

    Flávia Sousa, Pedro Fonte, Andreia Cruz in Recombinant Glycoprotein Production (2018)

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    Protocol

    Quantitation of ER Structure and Function

    The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin ...

    Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara in The Plant Endoplasmic Reticulum (2018)

  5. No Access

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    Isolation and Characterization of Endogenous RNPs from Brain Tissues

    Identification of physiological target RNAs and protein interactors bound to RNA-binding proteins is a key prerequisite to understand the underlying mechanisms of posttranscriptional expression control and RNA...

    Rico Schieweck, Foong yee Ang, Renate Fritzsche, Michael A. Kiebler in RNA Detection (2018)

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    Protocol

    Optogenetics in Stem Cell Research: Focus on the Central Nervous System

    Stem cell-based therapies of CNS disorders represent a promising approach in translational and regenerative medicine. Stem cell-based tissue replacement and regeneration would, for the first time, offer a caus...

    Johannes Boltze, Albrecht Stroh in Optogenetics: A Roadmap (2018)

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    A Method for Testing Random Spatial Models on Nuclear Object Distributions

    The cell nucleus is a structurally complex and dynamic organelle ensuring key biological functions. Complex relationships between nuclear structure and functions require a better understanding of the three-dim...

    Javier Arpòn, Valérie Gaudin, Philippe Andrey in Plant Chromatin Dynamics (2018)

  8. No Access

    Protocol

    Computer-Aided Drug Design Approaches to Study Key Therapeutic Targets in Alzheimer’s Disease

    Alzheimer’s Disease (AD) is one of the most common and complex age-related neurodegenerative disorders in elderly people. Currently there is no cure for AD, and available therapeutic alternatives only improve ...

    Agostinho Lemos, Rita Melo, Irina S. Moreira in Computational Modeling of Drugs Against Al… (2018)

  9. No Access

    Protocol

    Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

    Bacterial species identification is required in different disciplines and—depending on the purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular methods are the mainstay for bacte...

    Gabriele Margos, Isabell Notter, Volker Fingerle in Borrelia burgdorferi (2018)

  10. No Access

    Protocol

    Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

    Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations ...

    Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad in Cancer Stem Cells (2018)

  11. No Access

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    Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole-Mount Plant Tissue Using NucleusJ

    Image analysis is a classical way to study nuclear organization. While nuclear organization used to be investigated by colorimetric or fluorescent labeling of DNA or specific nuclear compartments, new methods ...

    Sophie Desset, Axel Poulet, Christophe Tatout in Plant Chromatin Dynamics (2018)

  12. No Access

    Protocol

    Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing

    High-throughput sequencing (HTS) is an effective tool for bacteriophage genome and its termini analysis. HTS technology parallelizes the sequencing process, producing thousands to millions of reads concurrentl...

    Xianglilan Zhang, Yahui Wang, Yigang Tong in Bacteriophages (2018)

  13. No Access

    Protocol

    Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay

    The family of inward rectifying potassium channels (Kir channels) plays crucial roles in the regulation of heart rhythms, renal excretion, insulin release, and neuronal activity. Their dysfunction has been att...

    Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi in Potassium Channels (2018)

  14. No Access

    Protocol

    Isolation and Purity Assessment of Membranes from Norway Spruce

    Gaining membrane vesicles from different plant species and tissue types is crucial for membrane studies. Membrane vesicles can be used for further purification of individual membrane types, and, for example, i...

    Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza in Plant Membrane Proteomics (2018)

  15. No Access

    Protocol

    Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels

    To study membrane protein structures using cryo-Electron Microscopy (cryo-EM), membrane proteins are usually extracted from cell membranes and solubilized in detergents. To restore the lipid bilayer environmen...

    Liguo Wang in Potassium Channels (2018)

  16. No Access

    Protocol

    Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

    This article will outline surveillance approaches for viral hemorrhagic fevers. Specific methods for surveillance of clinical samples will be emphasized. Separate articles will describe methods for surveillanc...

    Carolina Montoya-Ruiz, Juan David Rodas Ph.D. in Hemorrhagic Fever Viruses (2018)

  17. No Access

    Protocol

    Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma

    Bladder cancer diagnosis and surveillance is mainly based on cystoscopy and urine cytology. However, both methods have significant limitations; urine cytology has a low sensitivity for low-grade tumors, while ...

    Tibor Szarvas Ph.D., Péter Nyirády M.D., Ph.D., D.Sc. in Urothelial Carcinoma (2018)

  18. No Access

    Protocol

    Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming

    Dynamic reshuffling of the chromatin landscape is a recurrent theme orchestrated in many, if not all, plant developmental transitions and adaptive responses. Spatiotemporal variations of the chromatin properti...

    Clara Bourbousse, Moussa Benhamed, Fredy Barneche in Plant Chromatin Dynamics (2018)

  19. No Access

    Protocol

    Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models

    Over the last decades several mouse models of human hemoglobin disorders have been and continue to be generated. This chapter aims at describing various approaches to evaluate the global red blood cell propert...

    Marie Trudel, Josepha-Clara Sedzro in Erythropoiesis (2018)

  20. No Access

    Protocol

    Clinical Manifestations and Overall Management Strategies for Duchenne Muscular Dystrophy

    Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder that causes progressive weakness and wasting of skeletal muscular and myocardium in boys due to mutation of dystrophin. The structural integrit...

    Takeshi Tsuda in Duchenne Muscular Dystrophy (2018)

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