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    Autophagy in Adipocyte Differentiation

    Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which a...

    Zhipeng Tao, Longhua Liu, Louise D. Zheng in Autophagy in Differentiation and Tissue Ma… (2019)

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    The Detection Techniques for Autophagy-Associated Cell Death-Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry

    Autophagy is important in cellular homeostasis for the cell survival mechanism. Deficiency or excess of autophagy is generally related to some of diseases such as cancer and neurodegeneration. Although autopha...

    Melek Ozturk, Ozlem Dagdeviren Ozsoylemez in Autophagy in Differentiation and Tissue Ma… (2019)

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    Cloning of Autophagy-Related MicroRNAs

    Autophagy is a cellular survival pathway that is necessary for the degradation of cellular constituents such as long-lived proteins and damaged organelles. Conditions resulting in cellular stress such as starv...

    Deniz Gulfem Ozturk, Muhammed Kocak in Autophagy in Differentiation and Tissue Ma… (2019)

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    Methods for Monitoring Autophagy in Silkworm Organs

    In holometabolous insects, various larval organs are remodeled by autophagy during metamorphosis. Although moths and butterflies are among the first animal models in which this self-eating process was describe...

    Aurora Montali, Morena Casartelli in Autophagy in Differentiation and Tissue Ma… (2019)

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    Assays to Monitor Aggrephagy in Drosophila Brain

    Accumulation of ubiquitinated protein aggregates is a hallmark of most aging-related neurodegenerative disorders. Autophagy has been found to be involved in the selective clearance of these protein aggregates,...

    Anne-Claire Jacomin in Autophagy in Differentiation and Tissue Maintenance (2019)

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    Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, and ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

    Immunofluorescence is an invaluable technique widely used in cell biology. This technique allows visualization of the subcellular distribution of different target proteins or organelles, by specific recognitio...

    Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin in Autophagy in Differentiation and Tissue Ma… (2019)

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    Monitoring and Measuring Mammalian Autophagy

    Macroautophagy (autophagy) is a conserved lysosomal-based intracellular degradation pathway. Here, we present different methods used for monitoring autophagy at cellular level. The methods involve Atg8/LC3 det...

    Sujit K. Bhutia, Prakash P. Praharaj in Autophagy in Differentiation and Tissue Ma… (2019)

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    Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

    Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. ...

    Kiran Suhas Nilangekar in Autophagy in Differentiation and Tissue Ma… (2019)

  9. No Access

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    Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement

    Muscle wasting or cachexia is commonly associated with aging and many diseases such as cancer, infection, autoimmune disorders, and trauma. Decrease in muscle mass, or muscle atrophy, is often caused by dysfun...

    Kristyn Gumpper, Matthew Sermersheim in Autophagy in Differentiation and Tissue Ma… (2019)

  10. No Access

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    Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

    The Caco-2 colorectal cancer cell line is widely used as a model for intestinal differentiation and barrier function. These cells, upon reaching confluency, spontaneously differentiate into enterocyte-like cel...

    Sinem Tunçer, Sreeparna Banerjee in Autophagy in Differentiation and Tissue Maintenance (2019)

  11. No Access

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    Autophagy in Zebrafish Extraocular Muscle Regeneration

    Zebrafish extraocular muscles regenerate after severe injury. Injured myocytes dedifferentiate to a mesenchymal progenitor state and reenter the cell cycle to proliferate, migrate, and redifferentiate into fun...

    Alfonso Saera-Vila, Phillip E. Kish in Autophagy in Differentiation and Tissue Ma… (2019)

  12. No Access

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    Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells

    Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged s...

    Javad Alizadeh, Shahla Shojaei in Autophagy in Differentiation and Tissue Ma… (2019)

  13. No Access

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    Assessing Autophagy in the Leydig Cells

    Autophagy is an important intracellular degradation system which is implicated in many physiological and pathological processes. During autophagy, cytosolic constituents such as organelles and macromolecules a...

    Hui Gao, Chao Liu, Wei Li in Autophagy in Differentiation and Tissue Maintenance (2019)

  14. No Access

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    Porcine Cell-Free System to Study Mammalian Sperm Mitophagy

    A cell-free system using oocyte extracts is a valuable tool to study early events of animal fertilization and examine protein-protein interactions difficult to observe in whole cells. The process of postfertil...

    Won-Hee Song, Peter Sutovsky in Autophagy in Differentiation and Tissue Maintenance (2019)

  15. No Access

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    Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

    Autophagy is a fundamental cellular catabolic pathway mediating the recycling of cellular components. Autophagy has been implicated in pathogenesis of diverse diseases such as neurodegeneration and cancer. Due...

    Georgios Konstantinidis, Sonja Sievers in Autophagy in Differentiation and Tissue Ma… (2019)

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    Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

    Autophagy is an important cellular process for maintenance of quality and functionality of cells. This happens through repair and renewal of cellular components like proteins and mitochondria. Reduction in aut...

    Rohan S. Kulkarni, Manmohan S. Bajaj in Autophagy in Differentiation and Tissue Ma… (2019)

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    Visualization and Measurement of Multiple Components of the Autophagy Flux

    Autophagy is an intracellular degradation process that mediates the clearance of cytoplasmic components. As well as being an important function for cellular homeostasis, autophagy also promotes the removal of ...

    Tracey Evans, Robert Button, Oleg Anichtchik in Autophagy in Differentiation and Tissue Ma… (2019)

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    Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells Within Intact Tissues

    Here, we present Spatial Genomic Analysis (SGA), a quantitative single-cell transcriptional profiling method that takes advantage of single-molecule imaging of individual transcripts for up to a hundred genes....

    Antti Lignell, Laura Kerosuo (2018)

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    Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

    The niche plays critical roles in regulating functionality and determining regenerative outcomes of stem cells, for which establishment of favorable microenvironments is in demand in translational medicine. In...

    Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, Yan Jin (2018)

  20. No Access

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    Differentiation of Menstrual Blood Stem Cells into Keratinocyte-Like Cells on Bilayer Nanofibrous Scaffold

    Skin tissue engineering is a high-throughput technology to heal the wounds. Already, considerable advances have been achieved using stem cells for wound healing applications. Menstrual blood stem cell (MenSC) ...

    Shaghayegh Arasteh, Sara Katebifar, Reza Shirazi, Somaieh Kazemnejad (2018)

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