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  1. No Access

    Protocol

    Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast

    During transcription, the nascent transcript behind an elongating RNA polymerase (RNAP) can invade the DNA duplex and hybridize with the complementary DNA template strand, generating a three-stranded “R-loop” str...

    Aziz El Hage, David Tollervey in DNA Topoisomerases (2018)

  2. Protocol

    Erratum to: Functional Analysis of Membrane Proteins Produced by Cell-Free Translation

    Srujan Kumar Dondapati, Doreen A. Wüstenhagen, Stefan Kubick in Protein Engineering (2018)

  3. No Access

    Protocol

    Purification Method for Recombinant hG-CSF by Affinity Chromatography

    The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of...

    Bruna Samham Archangelo, Elisa Maria de Sousa Russo in Recombinant Glycoprotein Production (2018)

  4. No Access

    Protocol

    Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides

    Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including αA,B-crystallin polypeptides, is characterized by a conserved C...

    André-Patrick Arrigo in Chaperones (2018)

  5. No Access

    Protocol

    Modification of Selenoprotein mRNAs by Cap Tri-methylation

    Several selenoprotein mRNAs undergo 5′ cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m3 2,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunop...

    Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang in Selenoproteins (2018)

  6. No Access

    Protocol

    Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies

    Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formula...

    Flávia Sousa, Pedro Fonte, Andreia Cruz in Recombinant Glycoprotein Production (2018)

  7. No Access

    Protocol

    Quantitation of ER Structure and Function

    The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin ...

    Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara in The Plant Endoplasmic Reticulum (2018)

  8. No Access

    Protocol

    Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level

    Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we...

    Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, Stefan Hohmann in Glucose Transport (2018)

  9. No Access

    Protocol

    Isolation and Characterization of Endogenous RNPs from Brain Tissues

    Identification of physiological target RNAs and protein interactors bound to RNA-binding proteins is a key prerequisite to understand the underlying mechanisms of posttranscriptional expression control and RNA...

    Rico Schieweck, Foong yee Ang, Renate Fritzsche, Michael A. Kiebler in RNA Detection (2018)

  10. No Access

    Protocol

    A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics

    Traditional drug susceptibility methods can take several days or weeks of incubation between drug exposure and confirmation of sensitivity or resistance. In addition, these methods do not capture information a...

    Charlotte L. Hendon-Dunn, Stephen R. Thomas in Antibiotic Resistance Protocols (2018)

  11. No Access

    Protocol

    Optocapacitance Allows for Photostimulation of Neurons without Requiring Genetic Modification

    Optocapacitance is a novel technique that combines much of the power of optogenetics without requiring any transfection or genome modification in the organism or tissue of interest. It functions via the same p...

    Joao L. Carvalho-de-Souza, Jeremy S. Treger in Use of Nanoparticles in Neuroscience (2018)

  12. No Access

    Protocol

    Differential Proteomics Based on 2D-Difference In-Gel Electrophoresis and Tandem Mass Spectrometry for the Elucidation of Biological Processes in Antibiotic-Producer Bacterial Strains

    Proteomics based on 2D-Difference In Gel Electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) procedures can be considered a “gold standard” to determine quantitatively and comparatively protein abund...

    Giuseppe Gallo, Andrea Scaloni in Metabolic Network Reconstruction and Modeling (2018)

  13. No Access

    Protocol

    Optogenetics in Stem Cell Research: Focus on the Central Nervous System

    Stem cell-based therapies of CNS disorders represent a promising approach in translational and regenerative medicine. Stem cell-based tissue replacement and regeneration would, for the first time, offer a caus...

    Johannes Boltze, Albrecht Stroh in Optogenetics: A Roadmap (2018)

  14. No Access

    Protocol

    A Method for Testing Random Spatial Models on Nuclear Object Distributions

    The cell nucleus is a structurally complex and dynamic organelle ensuring key biological functions. Complex relationships between nuclear structure and functions require a better understanding of the three-dim...

    Javier Arpòn, Valérie Gaudin, Philippe Andrey in Plant Chromatin Dynamics (2018)

  15. No Access

    Protocol

    Recreating Intestinal Peristalsis in the Petri Dish

    Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic ref...

    Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez in Neurotrophic Factors (2018)

  16. No Access

    Protocol

    Computer-Aided Drug Design Approaches to Study Key Therapeutic Targets in Alzheimer’s Disease

    Alzheimer’s Disease (AD) is one of the most common and complex age-related neurodegenerative disorders in elderly people. Currently there is no cure for AD, and available therapeutic alternatives only improve ...

    Agostinho Lemos, Rita Melo, Irina S. Moreira in Computational Modeling of Drugs Against Al… (2018)

  17. No Access

    Protocol

    Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry

    Flow cytometry is a powerful tool for phenotypic and functional analyses of single immune cells. The increasing capability of flow cytometry technology has driven a more detailed understanding of immune cell s...

    Jianhong Zhang, Annie Bang, Stephen J. Lye in Sphingosine-1-Phosphate (2018)

  18. No Access

    Protocol

    Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

    Bacterial species identification is required in different disciplines and—depending on the purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular methods are the mainstay for bacte...

    Gabriele Margos, Isabell Notter, Volker Fingerle in Borrelia burgdorferi (2018)

  19. No Access

    Protocol

    Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

    Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations ...

    Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad in Cancer Stem Cells (2018)

  20. No Access

    Protocol

    Sequencing of Genomes from Environmental Single Cells

    Sequencing of single bacterial and archaeal cells is an important methodology that provides access to the genetic makeup of uncultivated microorganisms. We here describe the high-throughput fluorescence-activa...

    Robert M. Bowers, Janey Lee, Tanja Woyke in Next Generation Sequencing (2018)

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