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    Autophagy in Adipocyte Differentiation

    Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which a...

    Zhipeng Tao, Longhua Liu, Louise D. Zheng, Zhiyong Cheng (2017)

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    Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues

    An organoid is a cellular structure three-dimensionally (3D) cultured from self-organizing stem cells in vitro, which has a cell population, architectures, and organ specific functions like the originating org...

    Soojung Hahn, Jongman Yoo (2017)

  3. No Access

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    Decellularization of Liver and Organogenesis in Rats

    Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was ...

    Nana Shirakigawa, Hiroyuki Ijima (2017)

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    Decellularization and Recellularization of Cartilage

    Decellularization of cartilage enables the use of cartilage allografts or xenografts as natural scaffolds for repair and regeneration of injured cartilage. The preservation of the extracellular matrix ultrastr...

    Catherine A. Bautista, Bahar Bilgen (2017)

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    Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

    The extracellular matrix (ECM) is an important extracellular microenvironmental factor that regulates stem cell differentiation. The ECM is remodeled according to stem cell differentiation progression to preci...

    Takashi Hoshiba, Naoki Kawazoe, Guoping Chen (2017)

  6. No Access

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    Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

    Scaffolds, both natural and synthetic, used in tissue engineering provide mechanical support to cells. Tissue decellularization has been used to provide natural extracellular matrix scaffolds for tissue engine...

    Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, Omar S. Aljitawi (2017)

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    Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

    Sophisticated culturing conditions are required to grow cells in a three-dimensional (3D) environment. Cells then require a type of scaffold rich in proteins, growth factors, and signaling molecules that simul...

    Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri (2017)

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    Decellularized Bone Matrix Scaffold for Bone Regeneration

    Decellularized bone matrix is gaining a lot of attention as implantable biomaterials and/or biological scaffolds for bone tissue repair, and shows good clinical performance. This chapter describes the processi...

    Guobao Chen, Yonggang Lv (2017)

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    Gene Expression Array Analysis to Identify Candidate Tumor Suppressor Genes in Melanoma

    Melanoma is a complex multifactorial disease; therefore, a combination of various approaches is necessary to girt all aspects of its biology and identify the many different genes and factors involved in its et...

    Mitchell S. Stark, Vanessa F. Bonazzi (2017)

  10. No Access

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    Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying

    Microencapsulation of cells is beneficial for various biomedical applications, such as tissue regeneration and cell delivery. While a variety of techniques can be used to produce microspheres, electrohydrodyna...

    Mozhdeh Imaninezhad, Era Jain, Silviya Petrova Zustiak (2017)

  11. No Access

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    Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

    Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles...

    Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, Andrew J. Morris (2017)

  12. No Access

    Protocol

    In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate

    Sphingosine-1-phosphate (S1P) is a bioactive lipid that modulates migratory behavior of cells during embryonic development. In addition, S1P might promote tumor progression by enhancing migratory ability and i...

    Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, Sergio E. Alvarez (2017)

  13. No Access

    Protocol

    Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts

    Severe liver disease is the 12th leading cause of death in the USA, with organ transplantation often being the only viable option for treatment. However, due to the shortage of viable donor livers, it is estim...

    Yibin Chen, Sharon Geerts, Maria Jaramillo, Basak E. Uygun (2017)

  14. No Access

    Protocol

    Tracheal Cartilage Isolation and Decellularization

    Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to h...

    Zachary Galliger, Angela Panoskaltsis-Mortari (2017)

  15. No Access

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    Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering

    The reaggregation of dissociated cells to form organotypic structures provides an in vitro system for the analysis of the cellular interactions and molecular mechanisms involved in the formation of tissue arch...

    Megan K. Eldred, Leila Muresan, William A. Harris (2017)

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    Protocol

    Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids

    Cell remarkable ability to self-organize and rearrange in functional organoids has been greatly boosted by the recent advances in 3-D culture technologies and materials. This approach can be presently applied ...

    Tiziana A.L. Brevini, Elena F.M. Manzoni, Sergio Ledda, Fulvio Gandolfi (2017)

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    Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze-Thaw Cycles and Detergent Treatment

    Reliable decellularization techniques applicable to tendon tissue play a critical role in the field of current tissue engineering. Particularly, an application as three-dimensional culture model for in vitro r...

    Susanne Pauline Roth, Ina Erbe, Janina Burk (2017)

  18. No Access

    Protocol

    Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

    Organoid systems take advantage of the self-organizing capabilities of cells to create diverse multi-cellular tissue surrogates that constitute a powerful novel class of biological models. Clearly, the formati...

    Yoni Baert, Charlotte Rombaut, Ellen Goossens (2017)

  19. No Access

    Protocol

    Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

    Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vascul...

    Steven Skolasinski, Angela Panoskaltsis-Mortari (2017)

  20. No Access

    Protocol

    Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines

    Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), dia...

    Gary M. Reynolds, Barbara Visentin, Roger Sabbadini (2017)

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