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  1. No Access

    Protocol

    Purification Method for Recombinant hG-CSF by Affinity Chromatography

    The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of...

    Bruna Samham Archangelo, Elisa Maria de Sousa Russo in Recombinant Glycoprotein Production (2018)

  2. No Access

    Protocol

    Modification of Selenoprotein mRNAs by Cap Tri-methylation

    Several selenoprotein mRNAs undergo 5′ cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m3 2,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunop...

    Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang in Selenoproteins (2018)

  3. No Access

    Protocol

    Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies

    Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formula...

    Flávia Sousa, Pedro Fonte, Andreia Cruz in Recombinant Glycoprotein Production (2018)

  4. No Access

    Protocol

    Quantitation of ER Structure and Function

    The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin ...

    Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara in The Plant Endoplasmic Reticulum (2018)

  5. No Access

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    A Method for Testing Random Spatial Models on Nuclear Object Distributions

    The cell nucleus is a structurally complex and dynamic organelle ensuring key biological functions. Complex relationships between nuclear structure and functions require a better understanding of the three-dim...

    Javier Arpòn, Valérie Gaudin, Philippe Andrey in Plant Chromatin Dynamics (2018)

  6. No Access

    Protocol

    Computer-Aided Drug Design Approaches to Study Key Therapeutic Targets in Alzheimer’s Disease

    Alzheimer’s Disease (AD) is one of the most common and complex age-related neurodegenerative disorders in elderly people. Currently there is no cure for AD, and available therapeutic alternatives only improve ...

    Agostinho Lemos, Rita Melo, Irina S. Moreira in Computational Modeling of Drugs Against Al… (2018)

  7. No Access

    Protocol

    Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

    Bacterial species identification is required in different disciplines and—depending on the purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular methods are the mainstay for bacte...

    Gabriele Margos, Isabell Notter, Volker Fingerle in Borrelia burgdorferi (2018)

  8. No Access

    Protocol

    Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

    Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations ...

    Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad in Cancer Stem Cells (2018)

  9. No Access

    Protocol

    Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole-Mount Plant Tissue Using NucleusJ

    Image analysis is a classical way to study nuclear organization. While nuclear organization used to be investigated by colorimetric or fluorescent labeling of DNA or specific nuclear compartments, new methods ...

    Sophie Desset, Axel Poulet, Christophe Tatout in Plant Chromatin Dynamics (2018)

  10. No Access

    Protocol

    Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay

    The family of inward rectifying potassium channels (Kir channels) plays crucial roles in the regulation of heart rhythms, renal excretion, insulin release, and neuronal activity. Their dysfunction has been att...

    Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi in Potassium Channels (2018)

  11. No Access

    Protocol

    Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels

    To study membrane protein structures using cryo-Electron Microscopy (cryo-EM), membrane proteins are usually extracted from cell membranes and solubilized in detergents. To restore the lipid bilayer environmen...

    Liguo Wang in Potassium Channels (2018)

  12. No Access

    Protocol

    Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

    This article will outline surveillance approaches for viral hemorrhagic fevers. Specific methods for surveillance of clinical samples will be emphasized. Separate articles will describe methods for surveillanc...

    Carolina Montoya-Ruiz, Juan David Rodas Ph.D. in Hemorrhagic Fever Viruses (2018)

  13. No Access

    Protocol

    Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma

    Bladder cancer diagnosis and surveillance is mainly based on cystoscopy and urine cytology. However, both methods have significant limitations; urine cytology has a low sensitivity for low-grade tumors, while ...

    Tibor Szarvas Ph.D., Péter Nyirády M.D., Ph.D., D.Sc. in Urothelial Carcinoma (2018)

  14. No Access

    Protocol

    Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming

    Dynamic reshuffling of the chromatin landscape is a recurrent theme orchestrated in many, if not all, plant developmental transitions and adaptive responses. Spatiotemporal variations of the chromatin properti...

    Clara Bourbousse, Moussa Benhamed, Fredy Barneche in Plant Chromatin Dynamics (2018)

  15. No Access

    Protocol

    Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer

    The PI3K/AKT/mTOR signaling pathway shows frequent molecular and increased activity in cancer. Given its role in the regulation of cell growth, survival and metastasis, molecules within this pathway are prom...

    Anuja Sathe, Roman Nawroth in Urothelial Carcinoma (2018)

  16. No Access

    Protocol

    Defining the Pathways of Urogenital Schistosomiasis-Associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models

    Urogenital schistosomiasis (infection with Schistosoma haematobium) is a major cause of bladder carcinogenesis. However, the exact mechanisms of the sequelae leading up to the development of bladder cancer are po...

    Evaristus C. Mbanefo, Michael H. Hsieh M.D., Ph.D. in Urothelial Carcinoma (2018)

  17. No Access

    Protocol

    Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum

    Selenium is an essential element incorporated to different proteins with important biological functions in connection to antioxidant activity, cancer-protective properties, neurodegenerative pathologies, and p...

    Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera in Selenoproteins (2018)

  18. No Access

    Protocol

    Assays to Assess Arenaviral Glycoprotein Function

    Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-depe...

    Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly in Hemorrhagic Fever Viruses (2018)

  19. No Access

    Protocol

    Genome-Wide Mutagenesis in Borrelia burgdorferi

    Signature-tagged mutagenesis (STM) is a functional genomics approach to identify bacterial virulence determinants and virulence factors by simultaneously screening multiple mutants in a single host animal, and...

    Tao Lin, Lihui Gao in Borrelia burgdorferi (2018)

  20. No Access

    Protocol

    Two Photon Intravital Microscopy of Lyme Borrelia in Mice

    Two-photon intravital microscopy is a powerful tool that allows visualization of cells in intact tissues in a live animal in real time. In recent years, this advanced technology has been applied to understand ...

    Alexia A. Belperron Ph.D., Jialing Mao, Linda K. Bockenstedt in Borrelia burgdorferi (2018)

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