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In Cell 19F NMR for G-Quadruplex

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Handbook of Chemical Biology of Nucleic Acids

Abstract

G-quadruplexes are four-stranded DNA/RNA structures formed by G-rich sequences. Their structure and function in basic genetic processes are an active area of research in telomere, gene regulation, and functional genomics research. Investigation of G-quadruplex structures associated with biological events is therefore essential to understanding the functions of these molecules. Antibodies and some small molecules have been used to investigate DNA G-quadruplex structures in living cells. However, these methods cannot distinguish the detailed topologies of G-quadruplexes. Very recently, it was demonstrated that 19F NMR spectroscopy can distinguish different nucleic acid structures by the corresponding 19F signal. The simplicity and sensitivity of 19F NMR approach can be used to directly observe DNA G-quadruplex, RNA G-quadruplex, Hybrid DNA/RNA G-quadruplex in vitro and in living cells and quantitatively characterize the thermodynamic properties of the G-quadruplexes. The finding provides new insight into the structural behavior of G-quadruplex in living cells. These results open new avenues for the investigation of G-quadruplex structures in vitro and in living cells.

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Authors and Affiliations

  1. Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

    Yan Xu

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  1. Yan Xu
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Correspondence to Yan Xu .

Editor information

Editors and Affiliations

  1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan

    Naoki Sugimoto

Section Editor information

  1. Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia

    Janez Plavec

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Xu, Y. (2023). In Cell 19F NMR for G-Quadruplex. In: Sugimoto, N. (eds) Handbook of Chemical Biology of Nucleic Acids. Springer, Singapore. https://doi.org/10.1007/978-981-19-9776-1_11

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