Abstract
Single cell manipulation is one of the key methods in single cell technologies. A cell can be positioned to a designation at a specified velocity for performing tasks, such as cell evaluation and cell sorting. This chapter is started with the state-of-the-art technologies of single cell manipulation, which include manipulations with both micro-scale and macro-scale actuators. Manipulation with a macro-scale actuator has advantages of low-cost and easy-access, but is challenging to control due to scale difference of several orders. Therefore, the introduction will be followed by a review of single cell manipulations using a macro-scale actuator. Transfer function of the macro-to-micro manipulation is derived based on a mechanical model incorporating the deformation of microfluidic chip. The coefficients of the transfer function are determined based on experimental results. An advanced manipulation system with closed-loop control and an on-chip transmitter is presented with experimental results. Finally, applications on cell evaluation using macro-scale actuator will be discussed at the end of the chapter.
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Tsai, CH.D. (2022). Single Cell Manipulation Using Macro-scale Actuator. In: Santra, T.S., Tseng, FG. (eds) Handbook of Single-Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-8953-4_23
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DOI: https://doi.org/10.1007/978-981-10-8953-4_23
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