The Art of Therapeutic Antibody Discovery: Finding Them One Cell at a Time

Semenova, Touyana; Witas, Richard; Schroeder, Brianna L.; Bohn, Katherine; Voigt, Alexandria; Nguyen, Cuong Q.

doi:10.1007/978-981-10-4857-9_18-1

Touyana Semenova³,
Richard Witas⁴,
Brianna L. Schroeder³,
Katherine Bohn³,
Alexandria Voigt³ &
…
Cuong Q. Nguyen^3,4,5

370 Accesses

Abstract

The immune system is comprised of a complex heterogeneity of cells with dynamic interconnectivity eliciting a diverse array of immunological responses. An individual progeny that has originated from a single clone is programmed to function and behave differently than progenitor or sister cells. Bulk analysis can provide overarching patterns in such a complex system but fails to capture rare subsets and accurately characterize the true individuality of each cell. In the past decade, significant progress in single cell analysis has contributed to the discovery of broadly and highly potent neutralizing antibodies against infectious agents. The discovery of these unique and rare antibody-producing B cells is remarkably valuable for disease prevention, intervention, and vaccine design by mapping the antigenic epitopes. The discovery of these antibodies via single-cell technologies has led to promising diagnostic, treatment, and vaccine development for both viral infection and autoimmune diseases. In this review, we will explore the current advances in antibody discovery using single-cell analysis. We will discuss the major clinical and immunological impacts of antibody discovery for detection, diagnosis, and treatment of infectious diseases and autoimmunity due to the advancement of single-cell technologies.

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Acknowledgments

CQN is supported financially in part by PHS grants R03AI122182, R21AI130561, R01DE028544, and R01DE026450 from the National Institutes of Health. RW is supported by the T90/R90 Comprehensive Training Program in Oral Biology (NIDCR, T90 DE21990).

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Authors and Affiliations

Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA
Touyana Semenova, Brianna L. Schroeder, Katherine Bohn, Alexandria Voigt & Cuong Q. Nguyen
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA
Richard Witas & Cuong Q. Nguyen
Center of Orphaned Autoimmune Diseases, University of Florida, Gainesville, FL, USA
Cuong Q. Nguyen

Authors

Touyana Semenova
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Richard Witas
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Brianna L. Schroeder
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Katherine Bohn
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Alexandria Voigt
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Cuong Q. Nguyen
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Correspondence to Cuong Q. Nguyen .

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Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India
Tuhin Subhra Santra
Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan
Fan-Gang Tseng

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Semenova, T., Witas, R., Schroeder, B.L., Bohn, K., Voigt, A., Nguyen, C.Q. (2019). The Art of Therapeutic Antibody Discovery: Finding Them One Cell at a Time. In: Santra, T., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_18-1

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DOI: https://doi.org/10.1007/978-981-10-4857-9_18-1
Received: 15 February 2019
Accepted: 07 March 2019
Published: 28 March 2019
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-4857-9
Online ISBN: 978-981-10-4857-9
eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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