Liquid Extraction: BUME

Synonyms

Lipid extraction (Liquid extraction); Butanol/methanol (BUME)

Definitions

BUME refers to the chloroform-free, total lipid extraction from biofluids and tissue samples using a mixture of 1-butanol and methanol (BUME mixture) followed by a two-phase separation step for purification of the upper lipid extract. The method was developed to simplify and speed-up lipid extractions and facilitate automation while maintaining lipid results comparable to the Folch method.

Introduction

An important part of the workflow of lipid analysis is the extraction procedure. This step extracts and purifies the lipids from the biological sample and removes substances such as proteins, carbohydrates, and other polar metabolites that may interfere with lipid quantification and impair the long-term performance of the analytical system. While there has been an impressive development of automated and fast analytical methods, the extraction procedure is still often performed manually with traditional...