Encyclopedia of Lipidomics

Living Edition
| Editors: Markus R. Wenk

Derivatization in Gas Chromatography of Lipids

  • Tomáš Řezanka
  • Karolína Pádrová
  • Karel Sigler
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-7864-1_71-1
Derivatization is performed as part of gas chromatographic (GC) analysis of lipids primarily to extend the spectrum of substances that can be determined by this method. Derivatization in general converts less volatile and thermally labile substances into compounds that can be analyzed in the gaseous state. One of the most important methods is protection (derivatization) of polar groups such as –OH, –NH 2, –SH, or –COOH. Due to an elevated temperature in a gas chromatograph, the analysis of compounds having free polar groups either suffers from a poor division (formation of tailing peaks) like, e.g., in the case of free fatty acids, or the analysis completely fails, e.g., in the case of saccharides formed after the hydrolysis of glycopeptides (e.g., digalactosyldiacylglycerols) (Table 1).
Table 1

Names of conventional derivatizing agents



Common name














Methyl Ester Fatty Acid Methyl Ester Dimethyl Disulfide Silyl Ether Trifluoroacetic Anhydride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Springer Science+Business Media B.V. 2016

Authors and Affiliations

  • Tomáš Řezanka
    • 1
  • Karolína Pádrová
    • 2
  • Karel Sigler
    • 1
  1. 1.Institute of Microbiology, CASPragueCzech Republic
  2. 2.Department of BiotechnologyUniversity of Chemical Technology PraguePragueCzech Republic