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Encyclopedia of Lipidomics pp 1–11Cite as

Thin-Layer Chromatography/Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry of Lipids

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Synonyms

TLC/MALDI MS of Lipids

Introduction

Liquid chromatography (especially high-performance liquid chromatography (HPLC)) and planar chromatography (in particular thin-layer (TLC) or more recent high-performance thin-layer chromatography (HPTLC)) are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006).

TLC is a very common separation technique in organic chemistry and natural product chemistry, for instance, lipid chemistry where normally only small sample amounts are available. Although TLC is quite a simple and inexpensive method, it offers some important advantages in comparison to HPLC (Hahn-Deinstrop 2006; Fuchs et al. 2009a):

  1. 1.

    TLC is a very robust separation technique and is affected to a much lesser extent than HPLC by impurities that are potentially present in crude lipid extracts.

  2. 2.

    TLC uses always a completely new stationary phase. Therefore, there are no “memory” effects in contrast to HPLC. HPLC columns are much more expensive and are, thus, many...

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Abbreviations

9-AA:

9-Aminoacridine

Da:

Dalton

DESI:

Desorption electrospray ionization

DHB:

2,5-Dihydroxybenzoic acid

Er:YAG:

Erbium-doped yttrium-aluminum-garnet

ESI:

Electrospray ionization

FT:

Fourier transform

GSL:

Glycosphingolipids

HPLC:

High-performance liquid chromatography

IR:

Infrared

LC:

Liquid chromatography

LD:

Laser desorption

LPC:

Lysophosphatidylcholine

MALDI:

Matrix-assisted laser desorption and ionization

MS:

Mass spectrometry

m/z:

Mass over charge

NMR:

Nuclear magnetic resonance

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

PL:

Phospholipid

PNA:

Para-nitroaniline

ppm:

Parts per million

Rf:

Retardation factor

SM:

Sphingomyelin

S/N:

Signal to noise

THA:

2,4,6-Trihydroxyacetophenone

TLC:

Thin-layer chromatography

TOF:

Time-of-flight

UV:

Ultraviolet

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Authors and Affiliations

  1. University of Leipzig, Medical Department, Institute of Medical Physics & Biophysics, Härtelstr. 16/18, 04107, Leipzig, Germany

    Beate Fuchs

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  1. Beate Fuchs
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Correspondence to Beate Fuchs .

Editor information

Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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Fuchs, B. (2016). Thin-Layer Chromatography/Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry of Lipids. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_60-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_60-1

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  • Online ISBN: 978-94-007-7864-1

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