Encyclopedia of Lipidomics

Living Edition
| Editors: Markus R. Wenk

Thin-Layer Chromatography/Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry of Lipids

Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-7864-1_60-1

Synonyms

Introduction

Liquid chromatography (especially high-performance liquid chromatography (HPLC)) and planar chromatography (in particular thin-layer (TLC) or more recent high-performance thin-layer chromatography (HPTLC)) are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006).

TLC is a very common separation technique in organic chemistry and natural product chemistry, for instance, lipid chemistry where normally only small sample amounts are available. Although TLC is quite a simple and inexpensive method, it offers some important advantages in comparison to HPLC (Hahn-Deinstrop 2006; Fuchs et al. 2009a):
  1. 1.

    TLC is a very robust separation technique and is affected to a much lesser extent than HPLC by impurities that are potentially present in crude lipid extracts.

     
  2. 2.

    TLC uses always a completely new stationary phase. Therefore, there are no “memory” effects in contrast to HPLC. HPLC columns are much more expensive and are, thus,...

Keywords

Lipid Species MALDI Mass Spectrum MALDI Mass Spectrometric Fatty Acyl Residue Crude Lipid Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.University of Leipzig, Medical Department, Institute of Medical Physics & BiophysicsLeipzigGermany