Definitions
- Oleaginous alga:
-
Alga with a potential to produce more than 20% of neutral lipids per dry weight
- Lipid droplets:
-
Organelles surrounded by a phospholipid monolayer and composed of a core of neutral lipids. Thus, lipid droplets serve as a lipid storage organelle within the cell.
Chlamydomonas reinhardtii is a freshwater unicellular green alga from the family Chlamydomonadaceae, predominantly inhabiting soil. Furthermore, it is characterized by two anterior flagella, multiple mitochondria and one chloroplast (Merchant et al. 2007). Among all microalgae, it is the best characterized alga and a large variety of genetic tools are available, i.e., stable transformation with different selection markers is possible (Scranton et al. 2015; Liu and Benning 2013; Li-Beisson and Peltier 2013; Li-Beisson et al. 2015). Moreover, Chlamydomonas possesses a sexual cycle (Li-Beisson and Peltier 2013...
This is a preview of subscription content, log in via an institution.
Abbreviations
- DGDG:
-
Digalactosyldiacylglycerol
- DGTS:
-
Diacylglycerol-N,N,N-trimethylhomoserine
- MGDG:
-
Monogalactosyldiacylglycerol
- PC:
-
Phosphatidylcholine
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- PI:
-
Phosphatidylinositol
- PS:
-
Phosphatidylserine
- SQDG:
-
Sulfoquinovosyldiacylglycerol
- TAG:
-
Triacylglycerol
References
Boyle NR, Morgan JA. Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol. 2009;3(1):1.
Fan J, Andre C, Xu C. A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii. FEBS Lett. 2011;585(12):1985–91.
Giroud C, Gerber A, Eichenberger W. Lipids of Chlamydomonas reinhardtii. Analysis of molecular species and intracellular site (s) of biosynthesis. Plant Cell Physiol. 1988;29(4):587–95.
Guschina IA, Harwood JL. Algal lipids and their metabolism. In: Algae for biofuels and energy. Dordrecht: Springer; 2013. p. 17–36.
Haines TH. Do sterols reduce proton and sodium leaks through lipid bilayers? Prog Lipid Res. 2001;40(4):299–324.
Kong JN, Hardin K, Dinkins M, Wang G, He Q, Mujadzic T, et al. Regulation of Chlamydomonas flagella and ependymal cell motile cilia by ceramide-mediated translocation of GSK3. Mol Biol Cell. 2015;26(24):4451–65.
Légeret B, Schulz-Raffelt M, Nguyen HM, Auroy P, Beisson F, Peltier G, et al. Lipidomic and transcriptomic analyses of Chlamydomonas reinhardtii under heat stress unveil a direct route for the conversion of membrane lipids into storage lipids. Plant Cell Environ. 2016;39(4):834–47.
Li Y, Han D, Hu G, Sommerfeld M, Hu Q. Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii. Biotechnol Bioeng. 2010;107(2):258–68.
Li-Beisson Y, Peltier G. Third-generation biofuels: current and future research on microalgal lipid biotechnology. OCL. 2013;20(6):D606.
Li-Beisson Y, Beisson F, Riekhof W. Metabolism of acyl-lipids in Chlamydomonas reinhardtii. Plant J. 2015;82(3):504–22.
Liu B, Benning C. Lipid metabolism in microalgae distinguishes itself. Cur Op Biotechnol. 2013;24(2):300–9.
Markham JE, Lynch DV, Napier JA, Dunn TM, Cahoon EB. Plant sphingolipids: Function follows form. Curr Opin Plant Biol. 2013;16(3):350–7.
Martin-Creuzburg D, Merkel P. Sterols of freshwater microalgae: potential implications for zooplankton nutrition. J Plankton Res. 2016;38:865–77.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, et al. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 2007;318(5848):245–50.
Merchant SS, Kropat J, Liu B, Shaw J, Warakanont J. TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation. Cur Opin Biotechnol. 2012;23(3):352–63.
Nguyen HM, Cuiné S, Beyly-Adriano A, Légeret B, Billon E, Auroy P, et al. The green microalga Chlamydomonas reinhardtii has a single ω-3 fatty acid desaturase that localizes to the chloroplast and impacts both plastidic and extraplastidic membrane lipids. Plant Physiol. 2013;163(2):914–28.
Ohvo-Rekilä H, Ramstedt B, Leppimäki P, Slotte JP. Cholesterol interactions with phospholipids in membranes. Prog Lipid Res. 2002;41(1):66–97.
Porter JA, Young KE, Beachy PA. Cholesterol modification of hedgehog signaling proteins in animal development. Science. 1996;274(5285):255.
Rochaix J-D. The three genomes of Chlamydomonas. In: Discoveries in Photosynthesis. Dordrecht: Springer; 2005. p. 1047–55.
Salimova E, Boschetti A, Eichenberger W, Lutova L. Sterol mutants of Chlamydomonas reinhardtii: characterisation of three strains deficient in C24 (28) reductase. Plant Physiol Biochem. 1999;37(4):241–9.
Scranton MA, Ostrand JT, Fields FJ, Mayfield SP. Chlamydomonas as a model for biofuels and bio-products production. Plant J. 2015;82(3):523–31.
Siaut M, Cuine S, Cagnon C, Fessler B, Nguyen M, Carrier P, et al. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol. 2011;11(1):7.
Trémolières A. Glycerolipids: composition, biosynthesis and function in Chlamydomonas. In: The molecular biology of chloroplasts and mitochondria in chlamydomonas. Dordrecht: Springer; 1998. p. 415–31.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media B.V.
About this entry
Cite this entry
Popko, J. (2016). Lipid Composition of Chlamydomonas reinhardtii . In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_126-1
Download citation
DOI: https://doi.org/10.1007/978-94-007-7864-1_126-1
Received:
Accepted:
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-7864-1
Online ISBN: 978-94-007-7864-1
eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences