Encyclopedia of Lipidomics

Living Edition
| Editors: Markus R. Wenk

Gas Chromatography of Fatty Acid Methyl Esters: Isolation of Geometrical Isomers by Silver-Ion Chromatography

  • Cristina Cruz-Hernandez
  • Frédéric Destaillats
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-7864-1_102-1



Silver-ion chromatography is a very efficient method to purify fatty acid methyl ester (FAME) geometrical isomers before their resolution by GC. The method is based on the reversible formation of a weak charge-transfer complex between a silver ion and a double bond. A sigma bond can be formed between the silver ion and the pi electrons of double bonds; cis ethylenic double bonds form a stronger bond than trans bonds. The strength of the complex depends on the accessibility of the electrons in the orbitals as well as on steric hindrance (Cruz-Hernandez and Kramer 2007). Depending on the structure of the fatty acid moieties (chain length, number, configuration and position of double bonds), lipid species are retained for a specific time on the surface of the stationary phase.

Method based on silver-ion thin-layer chromatography (Ag+-TLC) in tandem with GC has been proved to be the best way to obtain a complete identification and analysis of the 18:1 FAME...


Double Bond Fatty Acid Methyl Ester Methyl Oleate Fatty Acid Ester Geometrical Isomer 
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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Nestlé Research CenterNestec LtdLausanneSwitzerland