Encyclopedia of Lipidomics

Living Edition
| Editors: Markus R. Wenk

Gas Chromatography of Fatty Acid Methyl Esters: Isolation of Geometrical Isomers by Silver-Ion Chromatography

  • Cristina Cruz-Hernandez
  • Frédéric Destaillats
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-7864-1_102-1

Synonyms

Argentation/Ag+

Silver-ion chromatography is a very efficient method to purify fatty acid methyl ester (FAME) geometrical isomers before their resolution by GC. The method is based on the reversible formation of a weak charge-transfer complex between a silver ion and a double bond. A sigma bond can be formed between the silver ion and the pi electrons of double bonds; cis ethylenic double bonds form a stronger bond than trans bonds. The strength of the complex depends on the accessibility of the electrons in the orbitals as well as on steric hindrance (Cruz-Hernandez and Kramer 2007). Depending on the structure of the fatty acid moieties (chain length, number, configuration and position of double bonds), lipid species are retained for a specific time on the surface of the stationary phase.

Method based on silver-ion thin-layer chromatography (Ag+-TLC) in tandem with GC has been proved to be the best way to obtain a complete identification and analysis of the 18:1 FAME...

Keywords

Double Bond Fatty Acid Methyl Ester Methyl Oleate Fatty Acid Ester Geometrical Isomer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Nestlé Research CenterNestec LtdLausanneSwitzerland