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Biomarkers in Osteoarthritis: Value of Proteomics

  • Cristina Ruiz-Romero
  • Patricia Fernández-Puente
  • Francisco J. Blanco
Reference work entry
Part of the Biomarkers in Disease: Methods, Discoveries and Applications book series (BDMDA)

Abstract

Osteoarthritis (OA) is the most common rheumatic pathology accounting for much of worldwide disability. OA is related to aging and may affect the structure of any joint tissue. The early stage accurate diagnosis of OA and the ability to monitor the efficacy of putative disease-modifying drugs remain among the essential unmet medical needs for this disease. Despite the prevalence of OA, the diagnostic methods currently available are limited and lack sensitivity. Furthermore, there is currently no effective therapy capable of slowing or reversing the pathological changes that occur in the joint during the disease process. Therefore, the discovery and application of novel, noninvasive, specific biochemical markers remain a priority. This chapter will focus on the current OA protein markers and the value of proteomics for the discovery and validation of useful candidates for early diagnosis and drug discovery.

Keywords

Biomarkers Osteoarthritis Proteomics Cartilage Synovium Synovial fluid Mass spectrometry 

List of Abbreviations

2-DE

Two-dimensional gel electrophoresis

COL2

Type II collagen

COMP

Cartilage oligomeric matrix protein

CS

Chondroitin sulfate

CTX-II

C-telopeptide region of COL2

DIGE

Differential in-gel electrophoresis

HA

Hyaluronic acid

HC

Healthy controls

iTRAQ

Isobaric tag for relative quantitation

K/L

Kellgren and Lawrence scale

KS

Keratan sulfate

LC

Liquid chromatography

MALDI-TOF/TOF

Matrix-assisted laser desorption/ionization-time of flight

MB

Magnetic beads

MMP

Matrix metalloproteinases

MS

Mass spectrometry

NAPPA

Nucleic acid programmable protein array

OA

Osteoarthritis

OARSI

Osteoarthritis Research Society International

PAGE

Polyacrylamide gel electrophoresis

PsA

Psoriatic arthritis

RA

Rheumatoid arthritis

SELDI

Surface-enhanced laser desorption/ionization

SF

Synovial fluid

SRM

Selected reaction monitoring

TIMP

Tissue inhibitor of matrix metalloproteinase

UF

Ultrafiltration

YKL-40

Cartilage glycoprotein 39

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Grupo de Proteómica, ProteoRed-PRB2/ISCIIIServicio de Reumatología. Instituto de Investigación Biomédica (INIBIC). Complexo Hospitalario Universitario A Coruña (CHUAC). Sergas. Universidad de A Coruña (UDC)A CoruñaSpain
  2. 2.Rheumatology DivisonINIBIC-Hospital Universitario A CoruñaA CoruñaSpain
  3. 3.Proteomics UnitINIBIC-Hospital Universitario A CoruñaA CoruñaSpain

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