Mouse Islet Isolation
Pancreatic islets transplantation is a therapeutic option for patients affected by type 1 diabetes. While it is always desirable to perform experiments directly with human islets, the limited availability of this precious tissue makes mouse islets a feasible option for experimental research in diabetes in many laboratories worldwide. This chapter summarizes from a practical perspective the main aspects of mouse islet isolation. We will discuss the technical factors that affect its success, as well as the practical issues (quality assessment, time, and cost) to take into account when selecting an islet isolation protocol. Finally, we will provide some guidelines on the necessary logistics for setting up this kind of methodology.
KeywordsIslet isolation Islet purification Animal models Quality assessment
This work was supported by a grant of the Basque Government for Groups of Excellence (IT-472-07) and a grant of the Department of Industry of the Basque Government (SAIO09-PE09BF01).
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