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Mouse Islet Isolation

  • Simona Marzorati
  • Miriam Ramirez-Dominguez
Reference work entry

Abstract

Pancreatic islets transplantation is a therapeutic option for patients affected by type 1 diabetes. While it is always desirable to perform experiments directly with human islets, the limited availability of this precious tissue makes mouse islets a feasible option for experimental research in diabetes in many laboratories worldwide. This chapter summarizes from a practical perspective the main aspects of mouse islet isolation. We will discuss the technical factors that affect its success, as well as the practical issues (quality assessment, time, and cost) to take into account when selecting an islet isolation protocol. Finally, we will provide some guidelines on the necessary logistics for setting up this kind of methodology.

Keywords

Islet isolation Islet purification Animal models Quality assessment 

Notes

Acknowledgements

This work was supported by a grant of the Basque Government for Groups of Excellence (IT-472-07) and a grant of the Department of Industry of the Basque Government (SAIO09-PE09BF01).

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© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of Pediatrics, Faculty of Medicine and OdontologyUniversity of the Basque Country, UPV/EHU, University Hospital CrucesLeioaSpain
  2. 2.β Cell Biology UnitS. Raffaele Scientific Institute, Diabetes Research Institute-DRIMilanItaly

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