Encyclopedia of Nanotechnology

Living Edition
| Editors: Bharat Bhushan

Nanotechnology Applications in Polymerase Chain Reaction (PCR)

  • Kuo-Sheng Ma
  • Yingnan Ma
  • Fred Chiou
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-6178-0_356-2

Synonyms

Definition

Polymerase chain reaction (PCR) is the most prevalent technology used in modern molecular biology research. Generally, it is a technique to amplify the amount of a piece of DNA with a specific sequence. Although the PCR technique is mature, improvement of its efficiency is still an emerging area of research. Recently, nanotechnology is getting more attention in this application. Several nanometer-sized materials such as carbon nanotubes, gold nanoparticles, quantum dots, and metal oxide nanoparticles have been employed.

Introduction

The polymerase chain reaction (PCR) technique is a series of chemical processes to exponentially amplify the number of a specific DNA sequence, producing hundreds of thousands of copies of DNA from a low original sample concentration. This revolutionary technique was first developed by Dr. Kary Mullis in 1983 [1]. Since then, it has been broadly utilized in medical, biomedical,...

Keywords

Polymerase Chain Reaction TiO2 Nanoparticles Polymerase Chain Reaction Technique Conventional Polymerase Chain Reaction Mercaptoacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Electrical Mechatronics EngineeringSouthern Polytechnic State UniversityMariettaUSA
  2. 2.Department of Engineering TechnologyWeber State UniversityOgdenUSA