Encyclopedia of Nanotechnology

Living Edition
| Editors: Bharat Bhushan

Structure and Stability of Protein Materials

  • Szu-Wen WangEmail author
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-6178-0_290-2

Synonyms

Definition

Protein-based materials are polymeric biomaterials comprising amino acid subunits that are connected together by peptide bonds. These materials are usually biomimetic, self-assemble into higher-order nanometer-scale architectures, and can interact with biological entities. Determination of their structure and stability is an important component of assessing their utility and function.

Protein-Based Nanomaterials

The control of architecture at the nanoscale is a challenge in which nature has been highly successful. Since genetic manipulation enables the definition of every monomer in a polymeric protein structure, giving far greater control than conventional chemical synthesis, one approach in material synthesis is the use of protein engineering to create biologically inspired materials [1]. By combining natural scaffolds, structural elements, and biologically reactive sites, materials with novel architectures and properties can be...

Keywords

Differential Scanning Calorimetry Circular Dichroism Spider Silk Protein Material Comprise Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. 1.
    DiMarco, R.L., Heilshorn, S.C.: Multifunctional materials through modular protein engineering. Adv. Mater. 24, 3923–3940 (2012)CrossRefGoogle Scholar
  2. 2.
    Kluge, J.A., Rabotyagova, U., Leisk, G.G., Kaplan, D.L.: Spider silks and their applications. Trends Biotechnol. 26, 244–251 (2008)CrossRefGoogle Scholar
  3. 3.
    Molino, N.M., Wang, S.W.: Caged protein nanoparticles for drug delivery. Curr. Opin. Biotech. 28, 75–82 (2014)CrossRefGoogle Scholar
  4. 4.
    Creighton, T.E.: Proteins: Structures and Molecular Properties, 2nd edn. Freeman, New York (1993)Google Scholar
  5. 5.
    Xie, J.M., Schultz, P.G.: Innovation: a chemical toolkit for proteins – an expanded genetic code. Nat. Rev. Mol. Cell Biol. 7, 775–782 (2006)CrossRefGoogle Scholar
  6. 6.
    Connor, R.E., Tirrell, D.A.: Non-canonical amino acids in protein polymer design. Polym. Rev. 47, 9–28 (2007)CrossRefGoogle Scholar
  7. 7.
    Greenfield, N.J.: Using circular dichroism spectra to estimate protein secondary structure. Nat. Protoc. 1, 2876–2890 (2006)CrossRefGoogle Scholar
  8. 8.
    Greenfield, N.J., Fasman, G.D.: Computed circular dichroism spectra for evaluation of protein conformation. Biochemistry 8, 4108–4116 (1969)CrossRefGoogle Scholar
  9. 9.
    Muller, S.A., Muller, D.J., Engel, A.: Assessing the structure and function of single biomolecules with scanning transmission electron and atomic force microscopes. Micron 42, 186–195 (2011)CrossRefGoogle Scholar
  10. 10.
    Dalmau, M., Lim, S., Chen, H.C., Ruiz, C., Wang, S.W.: Thermostability and molecular encapsulation within an engineered caged protein scaffold. Biotechnol. Bioeng. 101, 654–664 (2008)CrossRefGoogle Scholar
  11. 11.
    Greenfield, N.J.: Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions. Nat. Protoc. 1, 2527–2535 (2006)CrossRefGoogle Scholar
  12. 12.
    Ladbury, J.E., Doyle, M.L. (eds.): Biocalorimetry 2: Applications of Calorimetry in the Biological Sciences. Wiley, Hoboken (2004)Google Scholar
  13. 13.
    Sanchez-Ruiz, J.M.: Protein kinetic stability. Biophys. Chem. 148, 1–15 (2010)CrossRefGoogle Scholar
  14. 14.
    Muller, D.J., Dufrene, Y.F.: Atomic force microscopy as a multifunctional molecular toolbox in nanobiotechnology. Nat. Nanotechnol. 3, 261–269 (2008)CrossRefGoogle Scholar
  15. 15.
    Wirtz, D.: Particle-tracking microrheology of living cells: principles and applications. Annu. Rev. Biophys. 38, 301–326 (2009)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Chemical Engineering and Materials ScienceThe Henry Samueli School of Engineering, University of CaliforniaIrvineUSA