Abstract
Two glycan isoforms of transferrin (Tf), Tf-1 and Tf-2, were found in cerebrospinal fluid (CSF). Tf-1 concentration in CSF was reduced in idiopathic normal pressure hydrocephalus (iNPH) , an elderly dementia caused by abnormal metabolism of CSF, whereas Tf-2 concentration was not. The reduction of Tf-1 concentration was not found in Alzheimer’s disease, indicating that Tf-1 can distinguish iNPH from Alzheimer’s disease. Glycan analysis revealed that Tf-1 has unique biantennary asialo- and agalacto-complex N-glycans, which carry bisecting β1,4-GlcNAc and core α1,6-fucose. In contrast, Tf-2 has α2,6-sialylglycan like serum Tf, suggesting that Tf-2 is derived from serum. Tf-1, a glycoform unique to CSF, is speculated to be produced in brain tissue. Immunohistochemistry using anti-Tf antibody on brain tissues revealed that the choroid plexus, which produces CSF, was strongly stained by anti-Tf antibody. These results suggest that Tf-1 is secreted from the choroid plexus and its secretion is reduced by abnormal metabolism of CSF in iNPH.
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Acknowledgments
We thank Dr. Kenneth Nollet for reviewing the manuscript. This work was supported by the New Energy and Industrial Technology Development Organization (MG project); the Ministry of Health, Labor, and Welfare of Japan (grant number 2011-Nanchi-Ippan-018); the Japan Science and Technology Agency (grant numbers AS221Z00232F, AS231Z01053, 241FT0255, and 149); the Ministry of Education, Culture, Sports, Science, and Technology of Japan [a Grant-in-Aid for Scientific Research on Innovative Areas, grant number 23110002 (Deciphering sugar chain-based signals regulating integrative neuronal functions) and a Grant-in-Aid for Scientific Research, grant number 23590367].
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Hoshi, K. et al. (2014). A Glycan Marker for Idiopathic Normal Pressure Hydrocephalus. In: Endo, T., Seeberger, P., Hart, G., Wong, CH., Taniguchi, N. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54836-2_177-1
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DOI: https://doi.org/10.1007/978-4-431-54836-2_177-1
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