Living Reference Work Entry

Glycoscience: Biology and Medicine

pp 1-11

Date: Latest Version

Imaging Cell-Surface Glycans in Animals with Bioorthogonal Chemistry

  • Brendan J. BeahmAffiliated withDepartments of Chemistry, University of California Email author 
  • , Carolyn R. BertozziAffiliated withDepartments of Chemistry, University of CaliforniaDepartments of Molecular and Cell Biology, University of CaliforniaHoward Hughes Medical Institute


The myriad functions of cell-surface glycans have rendered them attractive targets for in vivo molecular imaging. Until very recently, methods for specifically visualizing this class of biomolecules were lacking. The advent of bioorthogonal chemistry, i.e., reactions among functional groups that do not interact with or interfere with biological systems, provided a platform for probing glycans on cells and in animals. Herein, the progression of bioorthogonal reaction development and use of these chemistries for glycan imaging are described.


Bioorthogonal Imaging In vivo Metabolic Zebrafish Azide Cyclooctyne Glycan Reporter