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Preparation and Characterization of Drug Liposomes by Ammonium Sulfate Gradient

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Liposome-Based Drug Delivery Systems

Part of the book series: Biomaterial Engineering ((BIOENG))

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Abstract

Liposomes have been extensively studied and widely used for drug delivery in the past several decades. Various types of liposomal formulations have been used in anticancer therapies due to their distinctive advantages: versatility, biocompatibility, and flexibility. Liposomal formulations have been considered promising and encouraging dosage forms for novel anticancer chemotherapies. A high and stable encapsulation efficiency is the basis and premise for the preparation of the liposomal formulations. An active loading methods mediated by sulfate gradient is included in this protocol with an anthracycline, epirubicin, as the model drug. Principle of the ammonium sulfate gradient method is related to low permeability of ammonium counterion, the sulfate, which also stabilizes the drugs of weak alkaline accumulation in the aqueous phase due to the aggregation and gelation of anthracycline sulfate salt. The procedure of liposome preparation by the ammonium sulfate gradient is described step by step, while further anticancer effects are also concisely stated in the protocol.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Engineering, Beijing Institute of Petrochemical Technology, Beijing, China

    Rui-Jun Ju

  2. School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China

    Xue-Tao Li

Authors
  1. Rui-Jun Ju
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  2. Xue-Tao Li
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Corresponding author

Correspondence to Rui-Jun Ju .

Editor information

Editors and Affiliations

  1. Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, Beijing, China

    Wan-Liang Lu

  2. Department of Pharmaceutics School of Pharmaceutical Science, Peking University, Beijing, China

    Xian-Rong Qi

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Ju, RJ., Li, XT. (2021). Preparation and Characterization of Drug Liposomes by Ammonium Sulfate Gradient. In: Lu, WL., Qi, XR. (eds) Liposome-Based Drug Delivery Systems. Biomaterial Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-49320-5_4

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