Synonyms
Short Description
Flow cytometry is a general method for rapidly analyzing large numbers of cells (usually 10,000 or more) individually using light scattering, fluorescence, and absorbance measurements. The power of this method lies both in the wide range of cellular parameters that can be determined and in the ability to obtain information on how these parameters are distributed in the cell population.
Information on the immune system is acquired using numerous established assays and procedures in animal or in vitro models which are generally referred to as tier testing. Tier I assays constitute a broad stroke analysis of chemical effects on humoral-mediated and cell-mediated immunity in animals (usually mice). Tier II tests are designed to assess specific target cells and responses affected by chemicals and to give insights into mechanisms of immunotoxicity. Flow cytometry is routinely used in tier II tests to assess effects of...
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Burchiel SW, Kerkvliet NL, Gerberick GF, Lawrence DA, Ladics GS (1997) Assessment of immunotoxicity by multiparameter flow cytometry. Fundam Appl Toxicol 38:38–54
Burchiel SW, Lauer FT, Gurulé D, Mounho BJ, Salas VM (1999) Uses and future applications of flow cytometry in immunotoxicity testing. Methods 19:28–35
Immunotoxicology Technical Committee (2001) Application of flow cytometry to immunotoxicity testing: summary of a workshop. Toxicology 163:39–48
Luster MI, Portier C, Pait DG et al (1993) Risk assessment in immunotoxicology. II. Relationships between immune and host resistance tests. Fundam Appl Toxicol 21:71–82
Putman E, van Loveren H, Bode G et al (2002) Assessment of the immunotoxic potential of human pharmaceuticals: a workshop report. Drug Inform J 36:417–427
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer-Verlag Berlin Heidelberg
About this entry
Cite this entry
Roman, D. (2016). Flow Cytometry Technique. In: Vohr, HW. (eds) Encyclopedia of Immunotoxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54596-2_577
Download citation
DOI: https://doi.org/10.1007/978-3-642-54596-2_577
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-54595-5
Online ISBN: 978-3-642-54596-2
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences