Encyclopedia of Immunotoxicology

2016 Edition
| Editors: Hans-Werner Vohr

Popliteal Lymph Node Assay

  • Raymond Pieters
Reference work entry
DOI: https://doi.org/10.1007/978-3-642-54596-2_1200

Synonyms

 PLNA

Short Description

The popliteal lymph node assay (PLNA) is in use to test the immunostimulatory capacity of low molecular weight compounds, in particular pharmaceuticals. Briefly, compounds are injected subcutaneously into the hind footpad of either mice or rats, and 6–8 days later the draining popliteal lymph node (PLN) is excised and compared with popliteal lymph nodes from vehicle-treated animals. Comparison is basically made between the weight and the cellularity of the nodes; the ratio of these parameters from compound-treated over vehicle-treated PLNs (designated the PLN index) indicates whether or not the compound is immunostimulatory. Immunostimulation is regarded as one of the possible prerequisites for pharmaceuticals and other compounds to activate and sensitize T cells and to induce systemic allergy or autoimmune-like derangements.

Characteristics

In the late 1970s and early 1890s, Gleichmann and colleagues realized that graft-versus-host (GvH) reactions...

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References

  1. Gleichmann H (1981) Studies on the mechanism of drug sensitization: T-cell-dependent popliteal lymph node reaction to diphenylhydantoin. Clin Immunol Immunopathol 18:203–211PubMedCrossRefGoogle Scholar
  2. Gleichmann E, Pal ST, Rolink AG, Radasziewicz T, Gleichmann H (1984) Graft versus host reactions: clues to the etiopathology of a spectrum of immunological diseases. Immunol Today 5:324–332PubMedCrossRefGoogle Scholar
  3. Kammüller ME, Thomas C, De Bakker JM, Bloksma N, Seinen W (1989) The popliteal lymph node assay in mice to screen for the immune dysregulating potential of chemicals-a preliminary study. Int J Immunopharmacol 11:293–300PubMedCrossRefGoogle Scholar
  4. Pieters R, Albers R (1999) Screening tests for autoimmune-related immunotoxicity. Environ Health Perspect 107:673–677PubMedCentralPubMedCrossRefGoogle Scholar
  5. Verdier F, Virat M, Descotes J (1990) Applicability of the popliteal lymph node assay in the Brown Norway rat. Immunopharmacol Immunotoxicol 12:669–677PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Institute for Risk Assessment Sciences (IRAS)UtrechtThe Netherlands