Encyclopedia of Immunotoxicology

2016 Edition
| Editors: Hans-Werner Vohr

Plaque-Forming Cell Assays

  • Gregory S. Ladics
Reference work entry
DOI: https://doi.org/10.1007/978-3-642-54596-2_1171


Short Description

The aim of such assays is to evaluate the ability of an individual to mount a humoral immune response (i.e., antibody response) to a particular antigen, typically sheep red blood cells (SRBC). Following exposure to SRBCs or other T-cell-dependent antigens, the generation of an antigen-specific antibody response requires the cooperation and interaction of several immune cell types: antigen-presenting cells (e.g., macrophages), T cells, and B cells. Thus, there are a number of steps in the process where alterations in the function of specific cells can impair the ability of B cells to produce antigen-specific antibody. Because of this complex interaction of cells, the quantification of the plaque-forming cell (PFC) response...

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.DuPont PioneerWilmingtonUSA