Plaque-Forming Cell Assays
The aim of such assays is to evaluate the ability of an individual to mount a humoral immune response (i.e., antibody response) to a particular antigen, typically sheep red blood cells (SRBC). Following exposure to SRBCs or other T-cell-dependent antigens, the generation of an antigen-specific antibody response requires the cooperation and interaction of several immune cell types: antigen-presenting cells (e.g., macrophages), T cells, and B cells. Thus, there are a number of steps in the process where alterations in the function of specific cells can impair the ability of B cells to produce antigen-specific antibody. Because of this complex interaction of cells, the quantification of the plaque-forming cell (PFC) response...
- National Research Council (1992) Biologic markers in immunotoxicology. National Academy Press, Washington, DC, pp 1–206Google Scholar