Encyclopedia of Membranes

Living Edition
| Editors: Enrico Drioli, Lidietta Giorno

Affinity Membranes

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-40872-4_8-1


Affinity separation methods rely on a “molecular recognition” phenomenon between species. A molecule, known as the ligand, is permanently bounded onto an inert matrix and specifically recognizes the molecule of interest, known as the ligate, that can be separated. The ligand can be a naturally occurring molecule, an engineered macromolecule, or a synthetic molecule linked to the matrix by covalent coupling. The ligand-ligate interaction is selective and reversible, enabling the separation and fine purification of biological substances such as proteins, peptides, and nucleic acids on the basis of its individual chemical structure or biological function (Wilson and Poole 2009).

Among the separation techniques based on the affinity method, affinity chromatography is the most widely used. Due to the limitation associated to the traditional affinity chromatography with porous bead-packed columns (i.e., limited flow rate by pore diffusion), the membrane-based separation...

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  1. Klein E (ed) (1991) Affinity membranes: their chemistry and performance in adsorptive separation processes. Wiley, New YorkGoogle Scholar
  2. Klein E (2000) Affinity membranes: a 10-year review. J Membr Sci 179:1–27CrossRefGoogle Scholar
  3. Wilson ID, Poole CF (2009) Handbook of methods and instrumentation in separation science, vol 1. Academic, BostonGoogle Scholar
  4. Zou H, Luo Q, Zhou D (2001) Affinity membrane chromatography for the analysis and purification of proteins. J Biochem Biophys Methods 49:199–240CrossRefGoogle Scholar

Authors and Affiliations

  1. 1.Institute on Membrane Technology, National Research Council of Italy, ITM-CNRNational Research Council of ItalyRende (CS)Italy