Encyclopedia of Membranes

Living Edition
| Editors: Enrico Drioli, Lidietta Giorno

Affinity Membranes for Purification of Enzymes

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-40872-4_1852-2
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Enzymes and Their Purification

Enzymes are large biological molecules that catalyze chemical interconversions. Most enzymes are proteins and they are highly selective catalysts. Since enzymes are selective for their substrates and speed up only specific reactions from among many possibilities, the types of enzymes determine the pathways and therefore affect the products of reactions. Hence, there is always the need for enzymes of different types to be separated and purified.

Affinity Membranes

Conventional membranes generally act like a sieve for separation, blocking substances larger than the membrane pores and passing substances smaller than the membrane pores. However, affinity membranes operate not only on the sieving mechanism but more importantly have selective binding capabilities, provided by various binding sites on the external and internal surfaces of the membranes. Thus, affinity membranes can separate and purify substances with the combined advantages of high selectivity...

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References

  1. Hou KC, Zaniewski R (1990) Purification of urokinase by combined cation exchanger and afinity chromatographic cartridges. J Chromatogr-Biomed Appl 525(2):297–306CrossRefGoogle Scholar
  2. Jain P, Vyas MK, Geiger JH, Baker GL, Bruening ML (2010) Protein purification with polymeric affinity membranes containing functionalized poly(acid) brushes. Biomacromolecules 11(4):1019–1026CrossRefGoogle Scholar
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Authors and Affiliations

  1. 1.Department of Civil & Environmental EngineeringNational University of Singapore, Faculty of EngineeringSingaporeSingapore