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Affinity Chromatographic Materials

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  • First Online:
Encyclopedia of Polymeric Nanomaterials

Synonyms

Affinity beads; Affinity matrices; Affinity resins

Definitions

Affinity signifies the degree to which a molecule associates with another molecule. Affinity chromatography takes advantage of this association to isolate and purify target molecules for the ligand of interest from mixtures. Functional materials used to perform affinity purification of the target molecules are termed as affinity chromatography materials (affinity matrices).

Introduction and Historical Background

Affinity chromatography, one of liquid chromatography techniques, is based on specific and reversible interactions found in biological systems such as antigen–antibody reactions and enzyme–substrate interactions. Affinity chromatography is a practical and useful method capable of selectively isolating and purifying a target molecule from crude mixtures, using a specific binding partner or a ligand [1, 2]. Cell lysates or chemical libraries have been used as crude mixtures containing target molecules for...

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References

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Author information

Authors and Affiliations

  1. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho Midori-ku, 226-8501, Yokohama, Kanagawa, Japan

    Vipul Gupta, Thiprampai Thamamongood, Satoshi Sakamoto, Hiroshi Handa & Yuki Yamaguchi

Authors
  1. Vipul Gupta
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  2. Thiprampai Thamamongood
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  3. Satoshi Sakamoto
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  4. Hiroshi Handa
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  5. Yuki Yamaguchi
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Corresponding author

Correspondence to Vipul Gupta .

Editor information

Editors and Affiliations

  1. Kyoto Institute of Technology R & D Center for Bio-Based Materials, Kyoto, Japan

    Shiro Kobayashi

  2. Max-Planck-Institut für Polymerforschung, Mainz, Germany

    Klaus Müllen

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Gupta, V., Thamamongood, T., Sakamoto, S., Handa, H., Yamaguchi, Y. (2014). Affinity Chromatographic Materials. In: Kobayashi, S., Müllen, K. (eds) Encyclopedia of Polymeric Nanomaterials. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36199-9_136-1

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  • DOI: https://doi.org/10.1007/978-3-642-36199-9_136-1

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Online ISBN: 978-3-642-36199-9

  • eBook Packages: Springer Reference Chemistry and Mat. ScienceReference Module Physical and Materials ScienceReference Module Chemistry, Materials and Physics

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