Encyclopedia of Polymeric Nanomaterials

Living Edition
| Editors: Shiro Kobayashi, Klaus Müllen

Affinity Chromatographic Materials

  • Vipul GuptaEmail author
  • Thiprampai Thamamongood
  • Satoshi Sakamoto
  • Hiroshi Handa
  • Yuki Yamaguchi
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-36199-9_136-1

Synonyms

Definitions

Affinity signifies the degree to which a molecule associates with another molecule. Affinity chromatography takes advantage of this association to isolate and purify target molecules for the ligand of interest from mixtures. Functional materials used to perform affinity purification of the target molecules are termed as affinity chromatography materials (affinity matrices).

Introduction and Historical Background

Affinity chromatography, one of liquid chromatography techniques, is based on specific and reversible interactions found in biological systems such as antigen–antibody reactions and enzyme–substrate interactions. Affinity chromatography is a practical and useful method capable of selectively isolating and purifying a target molecule from crude mixtures, using a specific binding partner or a ligand [1, 2]. Cell lysates or chemical libraries have been used as crude mixtures containing target molecules for...

Keywords

Affinity Chromatography Target Molecule Affinity Matrix Schistosoma Japonicum Miniemulsion Polymerization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Vipul Gupta
    • 1
    Email author
  • Thiprampai Thamamongood
    • 1
  • Satoshi Sakamoto
    • 1
  • Hiroshi Handa
    • 1
  • Yuki Yamaguchi
    • 1
  1. 1.Department of Biological Information, Graduate School of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan