Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Absorbance Spectroscopy: Overview

  • Paul Wormell
  • Alison Rodger
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_784-1

Synonyms

Definition

Most spectroscopic phenomena arise from the interaction of a molecule or other chemical species with one photon at a time. Multiphoton processes are less commonly encountered. The molecule may either absorb or scatter the photon; it may also emit a photon. The simplest form of spectroscopy is absorption, which measures how much electromagnetic radiation (e.g., light) of a given frequency is absorbed by a collection of molecules. If a molecule absorbs a photon of frequency ν, it increases its energy by
$$ \Delta E= h\nu = h c/\lambda $$
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References

  1. Atkins PW, de Paula J (2010) Atkins’ physical chemistry, 9th edn. Oxford University Press, OxfordGoogle Scholar
  2. Atkins PW, Friedman RS (2011) Molecular quantum mechanics, 5th edn. Oxford University Press, OxfordGoogle Scholar
  3. Hollas JM (2004) Modern spectroscopy, 4th edn. Wiley, ChichesterGoogle Scholar

Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.School of Science and HealthWestern Sydney UniversityPenrithAustralia
  2. 2.Department of Molecular SciencesMacquarie UniversitySydneyAustralia

Section editors and affiliations

  • Alison Rodger
    • 1
  1. 1.Department of Molecular Sciences, Macquarie UniversityNWSAustralia