Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Absorption Spectroscopy to Indicate Macromolecule Structural Changes

  • Alison Rodger
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_777-1

Synonyms

Definition

The UV-visible absorption spectrum of a biomolecule is the combination of the electronic transitions of all of its component parts or chromophores. The spectrum thus depends on the electronic structures of its component parts, which in turn depend on their environment. Thus, e.g., the absorbance of a tryptophan chromophore that is buried in the hydrophobic core of the protein will be at least slightly different from that of an exposed tryptophan. Two particularly useful applications of UV absorption spectroscopy to probe structural changes are outlined below.

Macromolecule Condensation

Measuring a UV-visible spectrum with an absorption spectrometer may be used to follow the condensation of a macromolecule sample into particles, though what is actually being probed is scattering of the light rather than absorption. A monotonic increase in absorbance signal outside the absorption bands of the molecules being...

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References

  1. Marky LA, Breslauer KA (1987) Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curves. Biopolymers 26:1601–1620CrossRefPubMedGoogle Scholar
  2. Meistermann I, Moreno V, Prieto MJ, Molderheim E, Sletten E, Khalid S, Rodger PM, Peberdy J, Isaac CJ, Rodger A, Hannon MJ (2002) Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers. Proc Natl Acad Sci U S A 99:5069–5074CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Department of Molecular SciencesMacquarie UniversitySydneyAustralia

Section editors and affiliations

  • Alison Rodger
    • 1
  1. 1.Department of Molecular Sciences, Macquarie UniversityNWSAustralia