Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Absorbance Spectroscopy: Quality Assurance

  • Paul Wormell
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_773-1

Synonyms

Definition

Modern spectrophotometers are usually accurate and reliable, but this should never be taken for granted, and there is a range of protocols that can be used to assess such parameters as wavelength accuracy (e.g., using holmium perchlorate solution or the sharp emission lines from a hydrogen or deuterium lamp), stray radiation, photometric accuracy (e.g., using potassium dichromate solution, noting the associated hazards), and baseline flatness. This is a routine requirement for an accredited testing laboratory, which may, for example, be required to comply with AS ISO/IEC 17025:2018, General Requirements for the Competence of Testing and Calibration Laboratories (Standards Australia: AS ISO/IEC 17025:2018(2018)), and is a requirement of Good Laboratory Practice (OECD 1998). It may also be essential for a forensic testing laboratory, in which all aspects of the laboratory’s testing practice may attract intense scrutiny, and...

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References

  1. ASTM International: ASTM E131-10 (2015) Standard terminology relating to molecular spectroscopy. ASTM International, West ConshohockenGoogle Scholar
  2. ASTM International: ASTM E1421-99 (2015) Standard practice for describing and measuring performance of Fourier Transform Mid-Infrared (FT-MIR) spectrometers: level zero and level one tests1. ASTM International, West ConshohockenGoogle Scholar
  3. ASTM International: ASTM E169-16 (2016) Standard practices for general techniques of ultraviolet–visible quantitative analysis. ASTM International, West ConshohockenGoogle Scholar
  4. ASTM International: ASTM E275-08 (2013) Standard practices for describing and measuring performance of ultraviolet, visible, and near-infrared spectrophotometers. ASTM International, West ConshohockenGoogle Scholar
  5. ASTM International: ASTM E925-09 (2014) Standard practice for monitoring the calibration of ultraviolet–visible spectrophotometers whose spectral slit width does not exceed 2 nm. ASTM International, West ConshohockenGoogle Scholar
  6. ASTM International: ASTM E958-13 (2013) Standard practice for measuring practical spectral bandwidth of ultraviolet–visible spectrophotometers. ASTM International, West ConshohockenGoogle Scholar
  7. Organisation for Economic Co-operation and Development (1998) OECD principles of good laboratory practice (as revised in 1997). OECD Environmental Health and Safety Publications, ParisGoogle Scholar
  8. Standards Australia: AS 3753-2001 (Reconfirmed) 28 October 2016 (2001) Recommended practice for chemical analysis by ultraviolet/visible spectrophotometry, 2nd edn. Standards Australia, SydneyGoogle Scholar
  9. Standards Australia: AS ISO/IEC 17025:2018 (2018) General requirements for the competence of testing and calibration laboratories, 3rd edn. Standards Australia, SydneyGoogle Scholar

Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.School of Science and HealthWestern Sydney UniversityPenrithAustralia

Section editors and affiliations

  • Alison Rodger
    • 1
  1. 1.Department of ChemistryUniversity of WarwickCoventryUK