Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

US-SOMO: Methods for Construction and Hydration of Macromolecular Hydrodynamic Models

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_292-1
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Synonyms

Definition

Macromolecular hydrodynamics concerns the measurement of biophysical parameters that describe the conformation of large biopolymers in solution; modeling of these parameters permits the testing of putative structural models.

Introduction

The hydrodynamics of biomacromolecules (e.g., proteins, nucleic acids, polysaccharides), as described by observables such as the translational diffusion coefficient (Dynamic Light Scattering), sedimentation coefficient (Sedimentation Velocity Analytical Ultracentrifugation), rotational relaxation time (NMR, Fluorescence Spectroscopy), and (Intrinsic Viscosity), can be measured accurately. However, the interpretation of these parameters in terms of solution macromolecular structure is not a straightforward task. This entry concerns a method for the construction of bead models to permit the computation of hydrodynamic (and related) parameters.

Bead modeling methods were pioneered in the late 1960s–early...

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Authors and Affiliations

  1. 1.Proteomics and Mass Spectrometry UnitIRCCS Ospedale Policlinico San Martino, Istituto Nazionale per la Ricerca sul CancroGenovaItaly
  2. 2.Department of Chemistry and BiochemistryUniversity of MontanaMissoulaUSA
  3. 3.School of Life Sciences, College of Medical, Veterinary and Life SciencesUniversity of GlasgowGlasgowUK

Section editors and affiliations

  • Stephen E. Harding
    • 1
  • Mary K. Phillips-Jones
    • 2
  1. 1.School of Biosciences, NCMH LaboratoryUniversity of NottinghamSutton BoningtonUK
  2. 2.National Centre for Macromolecular HydrodynamicsUniversity of NottinghamSutton BoningtonUK