US-SOMO: Methods for Construction and Hydration of Macromolecular Hydrodynamic Models
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Macromolecular hydrodynamics concerns the measurement of biophysical parameters that describe the conformation of large biopolymers in solution; modeling of these parameters permits the testing of putative structural models.
The hydrodynamics of biomacromolecules (e.g., proteins, nucleic acids, polysaccharides), as described by observables such as the translational diffusion coefficient (Dynamic Light Scattering), sedimentation coefficient (Sedimentation Velocity Analytical Ultracentrifugation), rotational relaxation time (NMR, Fluorescence Spectroscopy), and (Intrinsic Viscosity), can be measured accurately. However, the interpretation of these parameters in terms of solution macromolecular structure is not a straightforward task. This entry concerns a method for the construction of bead models to permit the computation of hydrodynamic (and related) parameters.
Bead modeling methods were pioneered in the late 1960s–early...
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