Electron Microscopy of Motor Structure and Possible Mechanisms
Structural analyses of substructures of the bacterial flagellum by the complementary use of cryoelectron microscopy and X-ray crystallography provide structural and mechanical insights into the mechanisms of how they can exert their functions.
The bacterial flagellum is supramolecular motility machinery consisting at least of three parts: the basal body, the hook, and the filament. Flagellar assembly begins with the basal body, followed by the hook, and finally the filament. Most flagellar proteins are translocated via a type III protein export apparatus into the central channel of the growing flagellar structure and assemble at the distal end of the structure with the help of a capping structure. Escherichia coli and Salmonella entericaare model organisms that have provided detailed insights into the structure, assembly, and function of the...