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Bacteria in Food and Beverage Production

  • Michael P. Doyle
  • Larry R. Steenson
  • Jianghong Meng

Abstract

Foods typically contain a variety of bacteria of which some may be beneficial, such as those preserving foods through products of fermentation, and others may be harmful by causing human illness or food spoilage. Lactic acid bacteria are among the most important groups of microorganisms used in food fermentations and are largely included in the genera Carnobacterium, Enterococcus, Lactobacillus, Lactococcus , Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus, and Weissella. The essential feature of lactic acid bacteria metabolism is efficient carbohydrate fermentation coupled to substrate-level phosphorylation. These bacteria can degrade a variety of carbohydrates, with lactic acid being the predominant end product. Many lactic acid bacteria also produce bacteriocins that have antimicrobial activity that is antagonistic to other bacteria, especially toward bacteria closely related to the bacteriocin-producing strain. Bacteriocins are peptides that are produced ribosomally by bacteria and released extracellularly.

Starter cultures, which are largely comprised of lactic acid bacteria, are food-grade microorganisms that are used to produce fermented foods of desirable appearance, body, texture, and flavor. Types of fermented foods for which commercial starter cultures are currently used include dairy products (cheese, sour cream, yogurt), meat products (sausages), and vegetable products (pickles, sauerkraut, olives). For starter cultures to be effective during food fermentations, they must dominate over naturally occurring microflora and produce the desired end products of fermentation. Many of the activities essential for food fermentations, including lactose metabolism, proteinase activity, oligopeptide transport, bacteriophage-resistance mechanisms, bacteriocin production and immunity, bacteriocin resistance, exopolysaccharide production, and citrate utilization, are encoded on plasmids harbored by lactic acid bacteria. Advances in molecular technology have enabled the construction of superior strains of starter cultures for food fermentations. Improved features of these strains include bacteriophage resistance, genetic stability, and reduced variation and unpredictability in performance.

Another application for beneficial microbes used in foods is adding probiotic microorganisms to provide a health benefit to consumers. Many beneficial health effects for probiotics have been reported and include protection against enteric pathogens, improved digestion by means of enzymes to metabolize otherwise indigestible food nutrients (e.g., lactase to hydrolyze lactose in lactose intolerant consumers), stimulation of the intestinal immune system, and improvement of intestinal peristaltic activity. Lactic acid bacteria are the most common types of probiotic microbes being used. Probiotics have been largely delivered in fermented foods such as yogurt and fermented milk products; however, growing consumer interest in probiotics is leading to using other types of foods such as fruit and vegetable juices, cereal-based products, and even ice cream, as delivery vehicles.

Fermented foods are an important part of the food processing industry and of many consumers’ diets and are largely produced by lactic acid bacteria that have been selected for their ability to produce desired products or changes in the food. Many advances have been made during the past decade in developing improved bacterial strains for starter culture application, which largely have been made possible through advances in molecular technology. The use of lactic acid bacteria to enhance the quality and safety of foods is a rapidly evolving field. With the discovery of new bacteriocins and the development of more efficient approaches to deliver them to foods, the importance of lactic acid bacteria in preserving and providing enhanced safety of food will continue to increase for the foreseeable future.

Keywords

Lactic Acid Bacterium Starter Culture Fermented Food Bacteriocin Production Fermented Sausage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Abe F, Ishibashi N, Shimamura (1995) Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglet. J Dairy Sci 78:2838–2846Google Scholar
  2. Aho M, Nuotio L, Nurmi E, Kiiskinen T (1992) Competitive exclusion of campylobacters from poultry with K-bacteria and Broilact. Int J Food Microbiol 15:265–275PubMedCrossRefGoogle Scholar
  3. Anonymous (2002) Guidelines for the evaluation of probiotics in food. Joint FAO/WHO working group report on drafting guidelines for the evaluation of probiotics in food. http://www.who.int/foodsafety/publications/fs_management/probiotics2/en/
  4. Axelsson L (1998) Lactic acid bacteria: classification and physiology. In: Salminen S, von Wright A (eds) Lactic acid bacteria—microbiology and functional aspects. Marcel Dekker, New York, pp 1–72Google Scholar
  5. Beuchat LR (1997) Traditional fermented foods. In: Doyle M, Beuchat L, Montville T (eds) Food microbiology—fundamentals and frontiers. ASM Press, Washington, DC, pp 629–648Google Scholar
  6. Breidt F Jr, McFeeters RF, Díaz-Muñiz I (2007) Fermented vegetables. In: Doyle M, Beuchat L (eds) Food microbiology—fundamentals and frontiers, 3rd edn. ASM Press, Washington, DC, pp 783–794Google Scholar
  7. Breukink E, de Kruiff B (2006) Lipid II as a target for antibiotics. Nat Rev Drug Discov 5:321–333PubMedCrossRefGoogle Scholar
  8. Buckenhuskes H (1993) Selection criteria for lactic acid bacteria to be used as starter cultures for various food commodities. FEMS Microbiol Rev 12:253–272CrossRefGoogle Scholar
  9. Chandan R, Shahani K (1993) Yoghurt. In: Hui Y (ed) Dairy science technology handbook. VCH, New York, pp 2–53Google Scholar
  10. Chebbi NB, Chander H, Ranganathan B (1977) Casein degradation and amino acid liberation in milk by two highly proteolytic strains of lactic acid bacteria. Acta Microbiol Pol 26:281–284PubMedGoogle Scholar
  11. Cogan T, Accolas J (1996) Dairy starter cultures. VCH, New YorkGoogle Scholar
  12. Collins-Thompson DL, Slade PJ, Goethals M (1991) Use of low molecular mass RNA profiles to identify lactic acid bacteria and related organisms associated with foods. Int J Food Microbiol 14:135–143PubMedCrossRefGoogle Scholar
  13. Cotter PD, Hill C, Ross RP (2005) Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 3:777–788PubMedCrossRefGoogle Scholar
  14. Daeschel M, Fleming H (1984) Selection of lactic acid bacteria for use in vegetable fermentations. Food Microbiol 1:303–313CrossRefGoogle Scholar
  15. Daly C, Fitzgerald GF, Davis R (1996) Biotechnology of lactic acid bacteria with special reference to bacteriophage resistance. Antonie Van Leeuwenhoek 70:99–110PubMedCrossRefGoogle Scholar
  16. De Ambrosini VM, Gonzalez S, Perdigon G, de Ruiz Holgado AP, Oliver G (1996) Chemical composition of the cell wall of lactic acid bacteria and related species. Chem Pharm Bull (Tokyo) 44:2263–2267CrossRefGoogle Scholar
  17. De Vos WM (1999) Gene expression systems for lactic acid bacteria. Curr Opin Microbiol 2:289–295PubMedCrossRefGoogle Scholar
  18. Deegan LH, Cotter PD, Hill C, Ross P (2006) Bacteriocins: biological tools for bio-preservation and shelf-life extension. Int Dairy J 16:1058–1071CrossRefGoogle Scholar
  19. Delves-Broughton J, Blackburn P, Evans RJ, Hugenholtz J (1996) Applications of the bacteriocin, nisin. Antonie Van Leeuwenhoek 69:193–202PubMedCrossRefGoogle Scholar
  20. Dinsmore PK, Klaenhammer TR (1995) Bacteriophage resistance in Lactococcus. Mol Biotechnol 4:297–314PubMedCrossRefGoogle Scholar
  21. Early R (1998) In: Early R (ed) The technology of dairy products, 2nd edn. Blackie, LondonGoogle Scholar
  22. Egan AF (1983) Lactic acid bacteria of meat and meat products. Antonie Van Leeuwenhoek 49:327–336PubMedCrossRefGoogle Scholar
  23. El-Nezami H, Ahokas J (1998) Lactic acid bacteria: an approach for detoxification of alfatoxins. In: Salminen S, von Wright A (eds) Lactic acid bacteria—microbiology and functional aspects. Marcel Dekker, New York, pp 359–368Google Scholar
  24. Galvez A, Abriouel H, Lopez RL, Omar NB (2007) Bacteriocin-based strategies for food biopreservation. Int J Food Microbiol 120:51–70PubMedCrossRefGoogle Scholar
  25. Gasson MJ (1990) In vivo genetic systems in lactic acid bacteria. FEMS Microbiol Rev 7:43–60PubMedGoogle Scholar
  26. Geisen R, Holzapfel WH (1996) Genetically modified starter and protective cultures. Int J Food Microbiol 30:315–324PubMedCrossRefGoogle Scholar
  27. Gilarova R, Voldrich M, Demnerova K, Cerovsky M, Dobias J (1994) Cellular fatty acids analysis in the identification of lactic acid bacteria. Int J Food Microbiol 24:315–319PubMedCrossRefGoogle Scholar
  28. Gilliland SE (1990) Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev 7:175–188.PubMedCrossRefGoogle Scholar
  29. Hammes WP, Tichaczek PS (1994) The potential of lactic acid bacteria for the production of safe and wholesome food. Z Lebensm Unters Forsch 198:193–201PubMedCrossRefGoogle Scholar
  30. Heng NCK, Tagg JR (2006) What’s in a name? Class distinction for bacteriocins. Nat Rev Microbiol 4(2). doi:10.1038/nrmicro1273-c1Google Scholar
  31. Henick-Kling T (1995) Control of malo-lactic fermentation in wine: energetics, flavour modification and methods of starter culture preparation. J Appl Bacteriol 79(Suppl):29S–37SGoogle Scholar
  32. Horvath P, Barrangou R (2010) CRISPR/Cas, the immune system of bacteria and archaea. Science 327:167–170PubMedCrossRefGoogle Scholar
  33. Jay J, Loessner MJ, Golden DA (2007) Modern food microbiology, 7th edn. Chapman & Hall, New York, 790 ppGoogle Scholar
  34. Jeppesen VF, Huss HH (1993) Antagonistic activity of two strains of lactic acid bacteria against Listeria monocytogenes and Yersinia enterocolitica in a model fish product at 5 degrees C. Int J Food Microbiol 19:179–186PubMedCrossRefGoogle Scholar
  35. Johnson M, Steele J (2007) Fermented dairy products. In: Doyle M, Beuchat L (eds) Food microbiology—fundamentals and frontiers, 3rd edn. ASM Press, Washington, DC, pp 767–782Google Scholar
  36. Jones RJ, Wescombe PA, Tagg JR (2011) Identifying new protective cultures and culture components for food biopreservation. In: Lacroix C (ed) Protective cultures, antimicrobial metabolites and bacteriophages for food and beverage biopreservation. Woodhead, Cambridge, UK, pp 3–26CrossRefGoogle Scholar
  37. Kandler O (1983) Carbohydrate metabolism in lactic acid bacteria. Antonie Van Leeuwenhoek 49:209–224PubMedCrossRefGoogle Scholar
  38. Klaenhammer TR (1991) Development of bacteriophage-resistant strains of lactic acid bacteria. Biochem Soc Trans 19:675–681PubMedGoogle Scholar
  39. Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12:39–86PubMedGoogle Scholar
  40. Klein G, Pack A, Bonaparte C, Reuter G (1998) Taxonomy and physiology of probiotic lactic acid bacteria. Int J Food Microbiol 41:103–125PubMedCrossRefGoogle Scholar
  41. Kuipers OP, de Ruyter PG, Kleerebezem M, de Vos WM (1997) Controlled overproduction of proteins by lactic acid bacteria. Trends Biotechnol 15:135–140PubMedCrossRefGoogle Scholar
  42. Lee B (1996) Bacteria-based processes and products. In: Lee B (ed) Fundamentals of food biotechnology. VEH, New York, pp 219–290Google Scholar
  43. Lee JH, Li X, O’Sullivan DJ (2011) Transcription analysis of a lantibiotic gene cluster from Bifidobacterium longum DJO10A. Appl Environ Microbiol 77:5879–5887PubMedCrossRefGoogle Scholar
  44. Leroy F, De Vuyst L (2010) Bacteriocins of lactic acid bacteria to combat undesirable bacteria in dairy products. Aust J Dairy Technol 65:143–149Google Scholar
  45. Lewus CB, Kaiser A, Montville TJ (1991) Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl Environ Microbiol 57:1683–1688PubMedGoogle Scholar
  46. Luche F (1994) Fermented meat products. Food Res Int 27:299–308CrossRefGoogle Scholar
  47. Makela P, Schillinger U, Korkeala H, Holzapfel WH (1992) Classification of ropy slime-producing lactic acid bacteria based on DNA-DNA homology, and identification of Lactobacillus sake and Leuconostoc amelibiosum as dominant spoilage organisms in meat products. Int J Food Microbiol 16:167–172PubMedCrossRefGoogle Scholar
  48. Mayo B (1993) The proteolytic system of lactic acid bacteria. Microbiologia 9:90–106PubMedGoogle Scholar
  49. McKay LL, Baldwin KA (1990) Applications for biotechnology: present and future improvements in lactic acid bacteria. FEMS Microbiol Rev 7:3–14PubMedGoogle Scholar
  50. Morelli L, Vogensen FK, von Wright A (2004) Genetics of lactic acid bacteria. In: Salminen S, von Wright A, Ouwehand A (eds) Lactic Acid Bacteria: microbiological and functional aspects. Marcel Dekker, New York, pp 249–293Google Scholar
  51. Naidu AS, Bidlack WR, Clemens RA (1999) Probiotic spectra of lactic acid bacteria (LAB). Crit Rev Food Sci Nutr 39:13–126PubMedCrossRefGoogle Scholar
  52. Nurmi E, Rantala M (1973) New aspects of Salmonella infection in broiler production. Nature 241:210–211PubMedCrossRefGoogle Scholar
  53. Nurmi E, Nuotio L, Schneitz C (1992) The competitive exclusion concept: development and future. Int J Food Microbiol 15:237–240PubMedCrossRefGoogle Scholar
  54. Okereke A, Montville TJ (1991) Bacteriocin-mediated inhibition of Clostridium botulinum spores by lactic acid bacteria at refrigeration and abuse temperatures. Appl Environ Microbiol 57:3423–3428PubMedGoogle Scholar
  55. Ouwehand AC, Sondberg Svendsen L, Leyer G (2011) Probiotics: from strain to product. In: Kniebel W, Salminen S (eds) Probiotics and health claims. Wiley, W. Sussex, pp 37–47Google Scholar
  56. Poolman B, Kunji E, Hagting A, Juillard V, Konings W (1995) The proteolytic pathway of Lactococcus lactis. J Appl Bacteriol 79:65S–75SGoogle Scholar
  57. Pritchard GG, Coolbear T (1993) The physiology and biochemistry of the proteolytic system in lactic acid bacteria. FEMS Microbiol Rev 12:179–206PubMedCrossRefGoogle Scholar
  58. Rafter JJ (1995) The role of lactic acid bacteria in colon cancer prevention. Scand J Gastroenterol 30:497–502PubMedCrossRefGoogle Scholar
  59. Ray B (1996) Fundamental food microbiology. CRC Press, Boca RatonGoogle Scholar
  60. Ricke S, Zabala Diaz I, Keeton JT (2007) Fermented meat, poultry, and fish products. In: Doyle M, Beuchat L (eds) Food microbiology—fundamentals and frontiers, 3rd edn. ASM Press, Washington, DC, pp 795–816Google Scholar
  61. Rodriguez A, Vidal DR (1990) Genetics of lactic acid bacteria with special reference to lactococci. Microbiologia 6:51–64PubMedGoogle Scholar
  62. Rodriguez E, Tomillo J, Nunez M, Medina M (1997) Combined effect of bacteriocin-producing lactic acid bacteria and lactoperoxidase system activation on Listeria monocytogenes in refrigerated raw milk. J Appl Microbiol 83:389–395PubMedCrossRefGoogle Scholar
  63. Saarela MH (2011) Probiotic functional foods. In: Saarela M (ed) Functional foods: concept to product. Woodhead, Cambridge, UK, pp 425–488CrossRefGoogle Scholar
  64. Salminen S, Deighton M (1992) Lactic acid bacteria in the gut in normal and disordered states. Dig Dis 10:227–238PubMedCrossRefGoogle Scholar
  65. Salminen S, Salminen E (1997) Lactulose, lactic acid bacteria, intestinal microecology and mucosal protection. Scand J Gastroenterol Suppl 222:45–48PubMedGoogle Scholar
  66. Sanders ME (1988) Phage resistance in lactic acid bacteria. Biochimie 70:411–422PubMedCrossRefGoogle Scholar
  67. Schoeni JL, Doyle MP (1992) Reduction of Campylobacter jejuni colonization of chicks by cecum-colonizing bacteria producing anti-C. jejuni metabolites. Appl Environ Microbiol 58:664–670PubMedGoogle Scholar
  68. Sievers M, Teuber M (1995) The microbiology and taxonomy of Acetobacter europaeus in commercial vinegar production. J Appl Bacteriol (Suppl) 79:85S–95SGoogle Scholar
  69. Stackebrandt E, Teuber M (1988) Molecular taxonomy and phylogenetic position of lactic acid bacteria. Biochimie 70:317–324PubMedCrossRefGoogle Scholar
  70. Steele JL (1995) Contribution of lactic acid bacteria to cheese ripening. Adv Exp Med Biol 367:209–220PubMedCrossRefGoogle Scholar
  71. Steinkraus KH (1983) Handbook of indigenous fermented foods. Marcel Dekker, New YorkGoogle Scholar
  72. Stiles ME, Holzapfel WH (1997) Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36:1–29PubMedCrossRefGoogle Scholar
  73. Svetoch EA, Stern NJ (2010) Bacteriocins to control Campylobacter spp. in poultry – a review. Poult Sci 89:1763–1768PubMedCrossRefGoogle Scholar
  74. Thompson J (1988) Lactic acid bacteria: model systems for in vivo studies of sugar transport and metabolism in gram-positive organisms. Biochimie 70:325–336PubMedCrossRefGoogle Scholar
  75. Vandamme P, Pot B, Gillis M, de Vos P, Kersters K, Swings J (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438PubMedGoogle Scholar
  76. Verrips CT, van den Berg DJ (1996) Barriers to application of genetically modified lactic acid bacteria. Antonie Van Leeuwenhoek 70:299–316PubMedCrossRefGoogle Scholar
  77. Weber G, Steenson L, Delves-Broughton J (2008) Antimicrobial fermentate technology. In: Havkin-Frenkel D, Dudai N, van der Mheen H (eds) II International symposium on natural preservatives in food, feed, and cosmetics, vol 778, Acta Hort. International Society for Horticultural Science, Belgium, pp 79–84Google Scholar
  78. Whitehead HR, Cox GA (1935) The occurrence of bacteriophage in cultures of lactic streptococci. N Z J Sci Technol 16:319–320Google Scholar
  79. Wiedemann I, Breukink E, van Kraaij C, Kuipers OP, Bierbaum G, de Kruijff B, Sahl HG (2001) Specific binding of nisin to the peptidoglycan precursor lipid II combines pore formation and inhibition of cell wall biosynthesis for potent antibiotic activity. J Biol Chem 276:1772–1779PubMedGoogle Scholar
  80. Yan F, Polk DB (2010) Probiotics: progress toward novel therapies for intestinal diseases. Curr Opin Gastroenterol 26:95–101PubMedCrossRefGoogle Scholar
  81. Zhao S, Meng J, Zhao T, Doyle M (1995) Use of vaccine and biological control techniques to control pathogens in animals used for food. J Food Safety 15:193–199CrossRefGoogle Scholar
  82. Zhao T, Doyle MP, Harmon BG, Brown CA, Mueller PO, Parks AH (1998) Reduction of carriage of enterohemorrhagic Escherichia coli O157:H7 in cattle by inoculation with probiotic bacteria. J Clin Microbiol 36:641–647PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Michael P. Doyle
    • 1
  • Larry R. Steenson
    • 2
  • Jianghong Meng
    • 3
  1. 1.Department of Food Science & TechnologyUniversity of GeorgiaGriffinUSA
  2. 2.Dupont Nutrition and HealthNew CenturyUSA
  3. 3.Department of Nutrition and Food ScienceUniversity of MarylandCollege ParkUSA

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